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缺氧预适应通过蛋白激酶途径上调心肌细胞C促血管生成因子的表达(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2004年第1期
页码:
17-19
栏目:
实验研究
出版日期:
2004-01-01

文章信息/Info

Title:
Upregulation angiogenesis expression by hypoxia preconditioning via activation of protein kinase C
作者:
张 峰1;  梅其炳1;  曹云新2;  腾增辉1;  王剑波3;  朱肖星1
第四军医大学: 1.药理学教研室, 2.免疫学教研室, 3.药物研究所, 陕西 西安 710032
Author(s):
ZHANG Feng1;  MEI Qi-bing1;  CAO Yun-xin2;  TENG Zeng-hui1;  WANG Jian-bo3;  ZHU Xiao-xing1
1.Department of Pharmacoloy, 2.Department of Immunoloy, 3.Research Insitute of Drug, Forth Military Medical Unierity, Xi'an, Shaanxi 710032, China
关键词:
缺氧预适应; 心肌细胞; 血管内皮生长因子; 碱性成纤维细胞生长因子; 蛋白激酶C
Keywords:
hypoxia preconditioning;  cardiomyocyte;  vascular endothelial growth factor;  basic fibroblast growth factor;  protein kinae C
分类号:
R349.5
DOI:
-
文献标识码:
A
摘要:
目的:研究缺氧预适应(HPC)对心肌细胞血管内皮生长因子(VEGF)和碱性成纤维细胞生长因子表达的影响。方法:建立体外培养的新生大鼠心肌细胞HPC模型,免疫组织化学法结合计算机图像分析,研究 HPC对心肌细胞血管内皮生长因子(VEGF)和碱性成纤维细胞生长因子(bFGF)表达的影响以及蛋白激酶C(PKC)在此过程中的作用。结果:心肌细胞HPC后,VEGF和 bFGF表达显著增多,阳性染色吸光度值明显升高。PKC抑制剂chelerythrine chloride(Che) 可拮抗HPC促进心肌细胞表达VEGF和 bFGF的作用。结论:HPC可促进心肌细胞VEGF和bFGF的表达,此作用是通过激活 PKC途径介导的。
Abstract:
AIM: To invetiate the effect of hypoxia preconditioning(HPC) on the expression of vascular endothelial growth factor (VEGF) and basic fibroblat growth factor(bFGF). METHODS: HPC model of cltred neonatal rat cardiomyocytes was etablihed. Immunohistochemistry combined with computer image analysis was used to detect the expression of VEGF and bFGF under HPC. The effects of protein kinase(PKC) on this procedure was also investigated. RESULTS: VEGF and bFGF expressions in cardiomyocytes and the A vale of positive staining after HPC were maredly increaed. PKC antagonist chelerythrine chloride (Che) cold abolish the promotive effect of HPC on VEGF and bFGF expressions. CONCLUSION: HPC can upreglate VEGF and bFGF expressions in cultured neonatal rat cardiomyocytes via PKC activation.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2003-3-4.
更新日期/Last Update: 2004-01-01