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心肌缺血/再灌注时心肌细胞凋亡与心功能关系的实验研究(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2004年第1期
页码:
50-52
栏目:
临床研究
出版日期:
2004-01-01

文章信息/Info

Title:
Relationship between apoptosis and cardiac function in myocardially reperfused rat
作者:
李俊峡1; 贾国良2; 李佃贵3; 李振彬1; 虞国杰3; 蔡春林3
1.白求恩国际和平医院心血管内科,河北 石家庄 050082; 2.第四军医大学西京医院心血管内科,陕西 西安 710032; 3.河北医科大学内科学教研室,河北 石家庄 050031
Author(s):
LI Jun-xia1; JIA Guo-liang2; LI Dian-gui3; LI Zhen-bin1; YU Guo-jie3; CAI Chun-lin3
1.Department of Cardiovasology, Bethune International Peace Hospital, Shijiazhuang,Hebei 050082, China; 2. Department of Cardiology, Xijing Hospital, Xi'an, Shaanxi 71--32; 3. Department of Internal Medicine, Hebei Medical University, Shijiazhuang, Hebei
关键词:
缺血/再灌注损伤; 心功能; 细胞凋亡
Keywords:
myocardial reperfusion injury;  cardiac function;  apoptosis
分类号:
R541.4
DOI:
-
文献标识码:
A
摘要:
目的:探讨大鼠心肌缺血后不同再灌注时相心肌细胞凋亡的变化及其与心功能之间的关系。方法:60只大鼠随机分成假手术组,缺血 45min再灌注即刻、再灌注 2、4、8h组。以缺口末端标记法(TUNEL)标记凋亡细胞,左室插管监测左室功能变化情况。结果:心肌细胞凋亡随心肌缺血/再灌注不同时相而变化,其中细胞凋亡指数(AI)于再灌注后 2、4h最高(24±4;20±3);细胞凋亡与血肌酸磷酸肌酶(CK)呈正相关(r=0.92,P<0.05),与左室内压上升最大速率(+dp/dtmax)呈负相关(r=-0.81,P<0.05)。结论:心肌细胞凋亡随再灌注不同时相而变化,细胞凋亡可加重心肌缺血/再灌注损伤,进而加重心功能的损害。
Abstract:
AIM:To study changes in apoptosis and cardiac function with different durations of reperfusion after myocardial ischemia. METHODS: 60 rats were divided randomly into 5 groups, sham-operated (observing for 12h in operated control), 45 min of ischemia followed by 0 h of reperfusion (I 45 min R 0 h), I 45 min R 2 h, I 45 min R 4 h and I 45 min R8 h. The myocardial cell apoptosis was determined with terminal deoxynucleotidyl transferase-mediated dUTP-fluorescein nick end labeling(TUNEL) method. RESULTS: Cardimyocyte apoptosis varied with the duration of reperfusion.The apoptosis was positively correlated to CK(r=0.92,P<0.05),but negatively correlated to ventricular function (r=-0.81,P<0.05).CONCLUSION: The number of apoptatic myocytes varied with the duration of myocardial reperfusion. Changes in apoptosis may be invovled in the process of myocardial ischemia reperfusion injury.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2002-10-22
更新日期/Last Update: 2004-01-01