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异丙肾上腺素对心肌细胞内钙释放和肌浆网内钙容量的影响(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2004年第3期
页码:
197-200
栏目:
实验研究
出版日期:
2004-05-01

文章信息/Info

Title:
Effects of isoprenaline on intracellular Ca2+ release and sarcoplasmic reticulum Ca2+ content
作者:
沈建新12;  程和平3;  韩太真1
1.西安交通大学医学院生理学教研室，陕西 西安 710061；2. 汕头大学医学院生理学教研室，广东 汕头 515041；3.北京大学生命科学学院膜与膜生物工程国家实验室，北京 100871
Author(s):
SHEN Jian-xin12;  CHENG He-ping3;  HAN Tai-zhen1
1. Department of Physiology, Medical School of Xi'an Jiaotong University，Xi'an Shaanxi 710061 China; 2. Department of Physiology，Shantou University Medical College, Shantou, Guangdong 515041, China; 3. National Laboratory of Biomembrane and Membrane Biotechnology, College of Life Science, Peking University, Beijing 100871, China
关键词:
异丙肾上腺素; 肌浆网; 钙信号; 心肌细胞
Keywords:
isoprenaline;  sarcoplasmic reticulum;  Ca2+ signaling;  cardiac myocytes
分类号:
R363
DOI:
-
文献标识码:
A
摘要:
目的：心肌细胞膜上的β-肾上腺素受体的激活对兴奋-收缩耦联(ECC)过程有重要调节作用。本课题利用异丙肾上腺素(ISO, 1 μmol/L)激活β-肾上腺素受体从而研究其对源自心肌细胞肌浆网内钙释放(ECC的重要环节)和肌浆网内钙容量的影响，进而分析钙释放与钙容量之间的关系。方法：局部场刺激作用于成年大鼠心肌细胞，促进后者产生动作电位，进而诱发胞内钙瞬变(ACT)，由ACT可估测细胞内钙释放。肌浆网内钙容量则由咖啡因(20 mmol/L)诱发的钙瞬变(CCT)估测。实验结果均由Zeiss LSM-510激光共聚焦显微镜系统记录。结果：ISO作用下的ACT峰值为10.29±0.35（n=13）比正常情况下的5.74±0.27（n=18）高（P<0.01）。ISO作用下的CCT峰值为11.23±0.29（n=13）比正常情况下的7.26±0.24（n=18）高（P<0.01）。结论：ISO可明显地提高心肌细胞内钙释放和肌浆网内的钙容量。不管有无ISO存在，胞内该释放量总是只占肌浆网内钙容量的一部分。在正常情况下，心肌的钙释放量有较大的储备能力，且储备可因β-肾上腺素受体的激活而动员。
Abstract:
AIM: β-Adrenergic receptor stimulation exerts important regulation on cardiac excitation-contraction coupling. In this study, isoprenaline (ISO, 1 μmol/L) were employed as β-Adrenergic receptor stimulator to investigate its effects on SR Ca2+ content and SR Ca2+ release (which is the key process in excitation-contraction coupling.), and the relationship between them. METHODS: Local field stimulation was delivered to freshly isolated rat single cardiac myocytes to generate action-potential-induced Ca2+ transients (ACT) for assessing intracellular Ca2+ content. Results were recorded by confocal Ca2+ imaging with a Zeiss LSM-510 confocal microscopic system. RESULTS: The peak of ACT with ISO was greater than that under the control conditions (P<0.01)(in △F/F0, the normalized fluorescence, where F0 denotes the resting fluorescence): 10.29±0.35（n=13）versus 5.74±0.27 （n=18）. And the peak of CCT with ISO was greater than that under the control conditions （P<0.01）as well: 11.23±0.29（n=13）versus 7.26±0.24（n=18）. CONCLUTIONS: ISO increased both cardiac intracellular Ca2+ release and SR Ca2+ content graetly. Ca2+ release was always only a part of the releasable SR Ca2+ conrent, regardless the presence of ISO. Under the normal conditions, cardiac myocytes had great reserve for Ca2+ release, which could be actived by β-Adrenergic receptor stimulation.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2003-5-5
更新日期/Last Update: 2004-05-01