我们的网站为什么显示成这样？

可能因为您的浏览器不支持样式，您可以更新您的浏览器到最新版本，以获取对此功能的支持，访问下面的网站，获取关于浏览器的信息：

|本期目录/Table of Contents|

兔阿霉素心衰模型的建立(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2004年第5期
页码:
437-439
栏目:
基础研究
出版日期:
2004-09-01

文章信息/Info

Title:
The establishment of adriamycin-induced heart failure model in rabbit
作者:
张　静; 李庚山; 李国草; 周　青; 李文强
武汉大学人民医院心内科,湖北武汉430060
Author(s):
ZHANG Jing ;  LI Geng-shan ;  LI Guo-cao ;  ZHOU Qing ;  LI Wen-qiang
Department of Cardiology , Renmin Hospital , Wuhan University , Wuhan ,Hubei 430060 , China
关键词:
动物模型;  兔;  阿霉素;  心肌病;  心衰
Keywords:
animal model ;  rabbit ;  adriamycin ;  cardiomyopathy ;  heart failure
分类号:
R33 - 33
DOI:
-
文献标识码:
A
摘要:
目的:探讨静脉注射常规剂量阿霉素(Adriamycin , ADR或Doxorubicin , DXR) 建立兔心衰动物模型的可行性,为 研究心衰的发病机制和有效治疗措施提供理想的动物模型。方法:日本大耳白兔19 只,随机分成模型组( n = 12) 和 对照组( n = 7) 。模型组:注射ADR(1 mg/ kg ,用0. 9 % NaCl 注射液配制成1. 0 mg/ ml 溶液) ,每周2 次,共8 周;对照 组:注射相同体积0. 9 % NaCl 注射液。于末次注射停药后3 周,测量其体质量(BW) 、心室质量(VW) 、左室收缩压 (LVSP) 、左室舒张末压(LVEDP) 及最大压力上升及下降速度( ±d p/ d tmax) ,并作病理切片观察其组织学变化。结果: 与对照组相比,模型组动物VW/ BW明显增大,LVSP 明显降低,LVEDP 明显升高, ±d p/ d tmax则显著减小( P < 0. 01) , 病理学结果证实符合心肌病样改变。结论: :常规剂量多次静脉注射ADR 可显著损伤心脏的舒缩功能,是建立兔心 肌病及慢性充血性心力衰竭模型的一种简便可靠的方法。
Abstract:
AIM: To study the feasibility of establishment a rabbit of adriamycin-induced heart failure , provide a considerable model for investigation of mechanism and effective therapy in hear failure. METHODS : There are 19 rabbits were randomly divided into 2 groups : The model group ( n = 8) was intravenous injection of ADR at a dose of 1mg/ kg twice weekly for 8 weeks , the control group ( n = 7) received an equivalent volume of 0. 9 % saline alone. 3 weeks after the final injection , body weight (BW) ,ventricular weight (VW) ,left ventricular systolic pressure (LVSP) ,Left ventricular end2diastolic pressure (LVEDP) and ±d p/ d tmax were evaluated. Hearts were harvested after that and analyzed by histological hematoxylin2eosin staining. RESULTS : Compared to the control group , VW/ BW and LVEDP of the model group increased , LVSP and ±d p/ d tmax decreased. Pathological examination demonstrated that the cardiac muscular tissue of the model group were harmed , which characteristic consistent with cardiomyopathy. CONCLUSION: Chronic administration of intravenous ADR to rabbits harmed systolic and diastolic function of hearts ; it is a simple and reliable method to establish a model of cardiomyopathy and chronic congestive heart failure.

参考文献/References

[1 ] Smith HJ , Nattall A. Experimental models of heart failure[J ] . Cardiovasc Res ,1985(4) ,19 :181 - 186.

[2 ] Williams GJ , Elston J , Muir JR. A simplified technique for the production of experimental aortic regurgitation [ J ] . Cardiovasc Res , 1975 , 9(3) :440 - 442.

[3 ] Garcia R , Diebold S. Simple , rapid , and effective method of preducing aortocaval shunts in the rat [J ] . Cardiovasc Res , 1990 ,24 (5) :430 -432.

[4 ] Sabbah HN , Stein PD , Kono T , et al . A canine model of chronic heart failure produced by multiple sequential coronary microembolizations[J ] .Am J Physiol , 1991 ,260 (4) :1379 - 1384.

[ 5 ] McDonald KM, Francis GS , Carlyle PF , et al . Hemodynamic , left ventricular structural and hormonal changes after discrete myocardial damage in the dog[J ] . J Am Coll Cardiol , 1992 ,19 :460 - 467.

[6 ] 亢晓东,陈　河. 阿霉素对兔心功能及心肌一氧化氮合酶表达的影响[J ] . 实用癌症杂志, 2001 ,16(4) :377 - 378 ,402.

[7 ] Singal PK, Iliskovic N. Doxorubicin2induced cardiomyopathy [ J ] . N Engl J Med , 1998 ,339 (13) :900 - 905.

[ 8 ] Arnolda L , McGrath B , Cocks M, et al . Adriamycin cardiomyopathy in the rabbit : an animal model of low output cardiac failure with activation of vasoconstrictor mechanisms[J ] . Cardiovasc Res , 1985 ,19 (6) : 378 -382.

[9 ] Monnet E , Orton EC. A canine model of heart failure by intracoronary adriamycin injection : hemodynamic and energetic results [ J ] . J Card Fail , 1999 , 5(3) : 255 - 264.

备注/Memo

备注/Memo:
收稿日期：2004-1-12
更新日期/Last Update: 2004-09-01