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体外模拟心肌微环境下骨髓间充质干细胞向心肌样细胞分化的实验研究(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2004年第6期
页码:
501-504
栏目:
基础研究
出版日期:
2004-11-01

文章信息/Info

Title:
Experiments studys on the differentiation of bone mesenchymal stem cells into cardiomyogenic cells in simulative cardiomyocytes microenvironments
作者:
郭　军1; 林国生1; 汪　蕾1; 杨　波1; 郭　军2; 贾光宏2; 鲍翠玉2; 刘维新3
武汉大学人民医院: 1. 心内科, 3. 胸心外科,湖北武汉430060 ; 2. 咸宁学院心血管疾病研究所,湖北咸宁437100
Author(s):
GUO Jun1; LIN Guo-sheng1; WANG Lei1; YANG Bo1; GUO Jun2; JIA Guang-hong2; BAO Cui-yu2; LIU Wei-xin3
1. Department of Cardiology , 3. Department of Cardiothoracic Surgery , Renmin Hospital , Wuhan University , Wuhan , Hubei 430060 ; 2. Cardiovascular Research Institute , Xianning College , Xianning , Hubei 437100
关键词:
骨髓间充质干细胞;  心肌细胞;  分化
Keywords:
mesenchymal stem cells ;  cardiomyocytes ;  differentiation
分类号:
R 541. 4
DOI:
-
文献标识码:
A
摘要:
目的: 探讨粒细胞集落刺激因子( G2-CSF) 和干细胞因子(SCF) 预处理对骨髓间充质干细胞(MSCs) 向心肌细 胞分化的影响。方法: MSCs 均采用全骨髓法提取,通过换液传代逐渐纯化细胞,取传4 代的MSCs ,在共培养前用 DAPI 标记,心肌细胞在培养的第3 d 与MSCs 共培养。对照组:未施加任何干预的MSCs 与心肌细胞共培养;实验 组:联合使用G-CSF 和SCF 对大鼠在体动员,提取其MSCs 与心肌细胞共培养。共培养前测定心肌细胞的活力,在 共培养第3 、4 、5 d 用免疫荧光标记肌钙蛋白T , 分析DAPI-MSCs 向心肌细胞分化的百分率。结果: 在共培养的第 3 、4 、5 d ,G-CSF 和SCF 处理的DAPI-MSCs 的分化率分别为(1. 24 ±0. 16) %、(3. 46 ±0. 21) %、(7. 21 ±0. 11) % ,均 显著高于对照组的0 、(1. 27 ±0. 13) %、(1. 32 ±0. 25) %。结论: : G-CSF 和SCF 对MSCs 具有促分化作用。
Abstract:
AIM: To investigate the effect of pret reatment of Granulocyte Colony-Stimulating Factor ( G-CSF) and Stem Cell Factor (SCF) on the differentiation of mesenchymal stem cells(MSCs) into cardiomyogenic cells. METHODS: The MSCs were isolated primarily f rom bone marrow of adult rat , and purified by passage culture. The 4th passage of MSCs was labled by DAPI . Then cardiomyocytes that have been cultured for 3 days were cocultured with DAPI-MSCs . The cont rol group : The MSCs which have no intervention were cocultured with cardiomyocytes. The experiment group : The MSCs which have been pret reated by SCF and G-CSF were cocultured with cardiomyocytes. The activity of cardiomyocytes was detected before coculture. Troponin T was examined by immunocytochemical method in the 3th、4th 、5th day of coculture separately. Then we analyse the percentage of the differentiation of MSCs into cardiomyogenic cells. RESULTS: DAPI-MSCs pret reated by SCF and G-CSF can be induced to differentiate into cardiomyogenic cells with higher positive rate of differentiation[ (1.24 ±0. 16) %、(3. 46 ±0. 21) %、(7. 21 ±0. 11) % in the 3th、4th 、5th day separately ]than the cont rol group[0 、(1. 27 ±0. 13) %、(1. 32 ±0. 25) %] . CONCLUSION: G-CSF and SCF could induce MSCs to differentiate into more cardiomyogenic cells in simulative cardiomyocytes microenvironment s in vitro.

参考文献/References

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[ 7 ] Kocher AA , Schuster MD , Szbolcs ML , et al . Neovascularization of ischemic myocardium by human bone-marrow-derived angioblasts prevents cardio myocyte apoptosis ,reduces remodeling and improves cardiac function[J ] . Nat Med ,2001 ,7 (4) :430 - 436.

[8 ] 陈运贤,欧瑞明,钟雪云,等. 粒细胞集落刺激因子干预性治疗大鼠心肌梗死的实验研究[ J ] . 中国病理生理杂志, 2001 , 17(12) :1153 - 1155.

[ 9 ] Orlic D , Kajstura J ,Chimenti S , et al . Bone marrow cells regenerate infarcted myocardium[J ] . Nat ure ,2001 ,410 :701 - 705.

备注/Memo

备注/Memo:
收稿日期：2004-3-25
更新日期/Last Update: 2004-11-01