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骨髓间充质干细胞移植在心肌病大鼠体内存活、分化的实验研究(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2005年第1期
页码:
4-7
栏目:
基础研究
出版日期:
2005-01-05

文章信息/Info

Title:
An experimental study on the survival and differentiation of transplanted bone marrow mesenchymal stem cells in rats with cardiomyopathy
作者:
江德勤; 田杰; 白永虹; 张文; 张蕾; 朱静; 陈沅
重庆医科大学附属儿童医院，重庆 400014
Author(s):
JIANG Deqin;  TIAN Jie;  BAI Yonghong;  ZHANG Wen;  ZHANG Lei;  ZHU Jing;  CHEN Yuan
Children's Hospital, Chongqing University of Medical Sciences, Chongqin 400014
关键词:
骨髓间充质干细胞; 移植; 分化; 心肌病; 实验研究
Keywords:
bone marrow mesenchymal stem cells (MSCs);  transplantation;  differentiation;  cardiomyopathy;  experimental study
分类号:
R542.2
DOI:
-
文献标识码:
A
摘要:
目的 研究骨髓间充质干细胞（MSCs）移植在心肌病大鼠体内存活、分化的情况。方法 将雌性Wistar大鼠30只经腹腔注射阿霉素6次（总剂量15 mg/kg），建立心肌病的动物模型，在此模型的左室前壁采用微静脉注射法植入MSCs。8周后处死大鼠，取出心脏标本，做冰冻切片进行HE染色，观察植入的MSCs在病损心肌组织中的变化及免疫荧光检查观察植入MSCs心肌肌球蛋白重链（MHC）及心肌特有的连接蛋白（CX43）表达情况。结果 HE染色检查显示，植入的MSCs存活并有新生血管形成；免疫荧光检查显示植入的MSCs，表达MHC及CX43，但较宿主表达弱。结论 MSCs移植在心肌病大鼠体内心肌微环境中能存活，并向心肌细胞分化。
Abstract:
AIM To study bone marrow mesenchymal stem cells (MSCs) transplanted to global myocardium in rats with doxorubicininduced cardiomyopathy and to observe their survival and differentiation in damaged host myocardium. METHODS Cardiomyopathy was induced by intraperitoneal injection of doxorubicin (total dose 15 mg/kg for six times) in thirty female Wistar rats. MSCs were intravenously injected into the left ventricular myocardium. Eight weeks later, the hearts were excised for histological analysis to observe morphological changes in the injured myocardium and for immunohistochemical analysis to investigate the expressions of sarcomeric myosin heavy chain (MHC) and connexin 43(CX43) of MSCs. RESULTS HE staining showed that the implanted MSCs labeled with DAPI survived and angiogenesis was identified at the implantation site. Eight weeks after the transplantation, immunohistochemical analysis showed the expression of MHC and CX43 in the implanted MSCs. CONCLUSION Implanted MSCs could survive in damaged myocardium and differentiate into cardiomyocytes.

参考文献/References

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［3］ 吕铁伟, 田杰, 朱静,等. 骨髓间充质干细胞体内诱导分化为心肌细胞的初步实验观察［J］.重庆医科大学学报, 2003，28（4）:463-466.

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［13］Orlic D, Kajstura J, Chimenti S, et al. Mobilized bone marrow cells repair the infarcted heart, improving function and survival［J］. Proc Natl Acad Sci U S A, 2001, 98(18):10344-10349.

备注/Memo

备注/Memo:
收稿日期：2004-01-12.基金项目：国家自然科学基金资助项目（编号 30271381）;重庆市科委资助项目(编号:渝科发计字［2001］5440)
更新日期/Last Update: 2010-01-05