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血红素氧合酶-1在胰岛素对乳鼠心肌细胞保护机制中的作用(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2005年第2期
页码:
111-114
栏目:
基础研究
出版日期:
2005-03-05

文章信息/Info

Title:
Role of heme oxygenase1 in myocardial protective mechanism of insulin
作者:
李锦; 蒋晖; 毛建斌; 陈茂; 范忠才; 黄德嘉
四川大学华西医院心内科, 四川 成都 610041
Author(s):
LI Jin;  JIANG Hui;  MAO Jianbin;  CHEN Mao;  FAN Zhongcai;  HUANG Dejia
Department of Cardiology, West China Hospital of Sichuan University, Chengdu, Sichuan 610041, China
关键词:
胰岛素; 心肌细胞; 血红素氧合酶1; 缺氧/复氧损伤
Keywords:
insulin;  cardiomyocytes;  heme oxygenase1;  hypoxiareoxygenation injury
分类号:
Q463
DOI:
-
文献标识码:
A
摘要:
目的 探讨血红素氧合酶(HO)1在胰岛素对缺氧/复氧心肌细胞保护机制中的作用。方法 体外培养SD乳鼠心肌细胞，分为7组：常氧对照组，缺氧/复氧对照组，不同剂量胰岛素组（160、320、640、960 mU/L），胰岛素加原卟啉锌组（胰岛素640 mU/L、原卟啉锌10 μmol/L）。除常氧对照组外，其余各组均给于缺氧3 h，再复氧1 h处理。检测心肌细胞HO1蛋白表达、HO活性、细胞生存率及乳酸脱氢酶（LDH）值。结果 与缺氧/复氧对照组比较，各胰岛素组心肌细胞HO1蛋白表达显著增加，HO活性、细胞生存率显著增高，LDH显著降低。原卟啉锌能显著抑制HO活性，阻止胰岛素对心肌细胞的保护作用。结论 胰岛素能够增加缺氧/复氧心肌细胞HO1的表达，增高HO活性，从而减轻心肌细胞缺氧/复氧损伤。
Abstract:
AIM To explore the role of heme oxygense1(HO1) in the myocardial protection of insulin. METHODS Neonatal cardiomyocytes of SD rats cultured in vitro, and then the study was performed according to different groups: Normoxia; Hypoxiareoxygenation; Insulin(160, 320, 640, 960 mU/L); Insulin(640 mU/L) and Zinc Protoporphyrin IX (znpp,10 μmol/L). Except Normoxia, the other groups were subjected to 3 hours of hypoxia and 1 hour of reoxygenation. Expression of HO1，HO activity, cell viability and lactate dehydrogenase (LDH) were detected. RESULTS In comparison with control group, HO1 expression, HO activity and cell viability increase, however, LDH levels decreases in insulin groups. Znpp could inhibit HO activity and blockade the myocardial protective mechanism of insulin. CONCLUSION Insulin could reduce the hypoxiareoxygenation injury of cardiomyocytes by increasing HO1 expression and HO activity.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2004-06-17.
更新日期/Last Update: 2010-01-05