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β磷酸甘油诱导体外培养大鼠主动脉平滑肌细胞的钙化(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2005年第3期
页码:
238-240
栏目:
临床研究
出版日期:
2005-05-05

文章信息/Info

Title:
βglycerophosphate induced calcification of rat aortic vascular smooth muscle cells
作者:
李寰1; 贾国良1; 张荣庆1; 张玉顺1; 陶惠人2; 吕荣2; 王军2
第四军医大学西京医院：1.心脏内科，2.骨科, 陕西 西安 710032
Author(s):
LI Huan1;  JIA Guoliang1;  ZHANG Rongqing1;  ZHANG Yushun1;  TAO Huiren2;  L Rong2;  WANG Jun2
1. Department of Cardiology, 2. Department of orthopaedics,Xijing Hospital， Fourth Military Medical University, Xi′an,Shaanxi 710032
关键词:
平滑肌细胞; 体外;  钙化; 大鼠
Keywords:
vascular smooth muscle cell; in vitro;  calcification;  rats
分类号:
R541.1
DOI:
-
文献标识码:
A
摘要:
目的 研究β磷酸甘油对体外培养的大鼠主动脉平滑肌细胞的钙化作用。方法 采用组织块培养法体外培养大鼠主动脉平滑肌细胞。细胞分为钙化组和正常组。钙化组加入10 mmol/L β磷酸甘油,0.1 μmol/L胰岛素及50 μg/L维生素Ｃ（钙化培养基）诱导细胞钙化，继续培养14 d。茜素红S染色观察钙结节计数并测定细胞钙沉积含量。采用四唑盐比色法(MTT)测量细胞增殖。结果 钙化组钙结节计数为（7.4±3.8）％，较正常组（4.3±1.9）％显著增加（P<0.05）；细胞钙沉积含量为（5.4±0.4) mmol/g蛋白, 较正常组（1.2±0.5）mmol/g蛋白显著增加（P<0.05）。MTT检测细胞增殖,钙化组细胞增殖为0.071±0.011, 较正常组0.040±0.009明显增加(P<0.01)。结论 β磷酸甘油能诱导体外培养的大鼠主动脉平滑肌细胞的钙化，钙化能促进平滑肌细胞的增殖。
Abstract:
AIM To study calcification of rat aortic vascular smooth muscle cells (VMSC) induced by βglycerophosphate in vitro. METHODS VMSC was cultured in vitro by tissue explant. All cells were divided into calcification group and normal group. In calcification group, 10 mmol/L βglycerophosphate, 0.1 μmol/L insulin and 50 μg/L Vitamin C were added to induce VSMC calcification. All groups were continuously treated for 14 days. Alizarin red S staining was applied to count the calcium nodus, and cell calcium depositions were measured. VSMC proliferation was measured by tetrazolium salt (MTT) methord. RESULTS The count of calcium nodus in calcification group was (7.4±3.8)％, which were significantly increased compared to normal group（4.3±1.9）％, (P<0.01). Cell calcium depositions in calcification group were (5.4±0.4) mmol/g protein, compared to normal group (1.2±0.5) mol/g protein, were significantly increased, and had reached a statistics difference (P<0.01). VSMC proliferation in calcification group was (0.071±0.011), compared to normal group (0.040±0.009), was significantly increased, and had reached statistics difference (P<0.01). CONCLUSION βglycerophosphate can induced calcification of rat aortic VSMC in vitro. Calcification can promote VSMC proliferation.

参考文献/References

［1］ Blumenthal HT, Lansing AI, Wheeler PA. Calcification of the media of the human aorta and its relationship to intimal arteriosclerosis, aging and disease［J］. Am J Pathol, 1994,20:665-687.

［2］ Fitzgerald PJ, Ports TA, Yock PG. Contribution of localized calcium deposits to dissection after angioplasty: an observational study using intravascular ultrasound［J］. Circulation, 1992, 86:64-70.

［3］ Shioi A, Nishizawa Y, Jono S, et al. βGlycerophosphate accelerates calcification in cultured bovine vascular smooth muscle cells［J］. Arterioscler Thromb Vasc Biol, 1995,15:2003-2009.

［4］ Jono S, Mckee MD, Murry CE, et al. Phosphate regulation of vascular smooth muscle cell calcification［J］. Circ Res, 2000,87:e10-e17.

［5］ Proudfoot D, Skeppe JN, Hegi L, et al. Apoptosis regulates human vascular calcification in vitro: Evidence for initiation of vascular calcification by apoptotic bodies［J］. Circ Res, 2000, 87:1055-1066.

备注/Memo

备注/Memo:
收稿日期：2004-12-30.
更新日期/Last Update: 2010-01-05