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呋喃二氢吡啶二羧酸酯对血管紧张素Ⅱ诱导血管平滑肌细胞增殖的抑制作用(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2005年第4期
页码:
341-344
栏目:
基础研究
出版日期:
2005-09-05

文章信息/Info

Title:
Inhibitory effects of diethyl2,6diethyl4furnyl, 4dihydropyridine3, 5 dicarboxylate on proliferation of cultured vascular smooth muscle cells induced by angiotension II
作者:
张殿新1; 李兰荪1; 贾国良1; 任雨笙2; 刘兵1
1. 第四军医大学西京医院心内科，陕西 西安 710032; 2. 第二军医大学长征医院心内科，上海 200003
Author(s):
ZHANG Dianxin1; LI Lansun1; JIA Guoliang1; REN Yusheng1; LIU Bing1
1. Department of Cardiology，Xijing Hospital, Fourth Military Medical University, Xi’an,Shaanxi 710032,China; 2. Department of Cardiology，Changzheng Hospital, Second Military Medical University, Shanghai 200003,China
关键词:
呋喃二氢吡啶二羧酸酯; 血管平滑肌细胞; 增殖;  血管紧张素
Keywords:
diethyl2; 6diethyl4furnyl;  4dihydropyridine3;  5 dicarboxylate;  vascular smooth muscle cells;  proliferation; angiotension II
分类号:
R541.7；R331.38
DOI:
-
文献标识码:
A
摘要:
目的 观察呋喃二氢吡啶二羧酸酯（EFDP）对血管紧张素II（AngⅡ）诱导血管平滑肌细胞（VSMC）增殖的影响。方法 采用培养的兔VSMC，应用3HTdR掺入法，观察在AngII促进VSMC增殖过程中，EFDP对VSMC DNA合成的影响及其时间效应。结果 AngII可促进处于静止状态的兔VSMC的 DNA合成，36 h时细胞DNA的合成达到高峰。EFDP对AngII诱导的VSMC增殖有显著的抑制作用，并呈现出明显的浓度依赖关系，随着EFDP浓度的升高，VSMC增殖的抑制率也逐渐增加，36 h时，78.40 μmol/L 的EFDP对VSMC增殖的抑制率为21.37%。结论 EFDP可抑制AngII诱导兔VSMC的增殖，并存在一定的量效依赖关系及时间反应性。
Abstract:
AIM To investigate effects of diethyl2,6diethyl4furnyl, 4dihydropyridine3, 5 dicarboxylate (EFDP) on proliferation of cultured vascular smooth muscle cells(VSMCs) stimulated by angiotension II(Ang II). METHODS The rabbit VSMCs were cultured and the inhibitory effect of EFDP on DNA synthesis in vascular smooth muscle cells stimulated by Ang II were assayed at different times with different concentrations of EFDP by using 3HTdR incorporation in vitro. RESULTS Ang II could significantly promote DNA synthesis in quiescent rabbit VSMCs, with a maximal response at 36th h. EFDP significantly inhibited DNA synthesis of VSMCs in a dosedependent manner, with a maximal response at a concentration of 78.40 μmol/L and at 36th h when the inhibitory rate was 21.37%. CONCLUSION EFDP significantly inhibits DNA synthesis of ribbit VSMCs induced by Ang II in a dose and time dependent manner.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2004-12-10.
更新日期/Last Update: 2010-01-05