我们的网站为什么显示成这样？

可能因为您的浏览器不支持样式，您可以更新您的浏览器到最新版本，以获取对此功能的支持，访问下面的网站，获取关于浏览器的信息：

|本期目录/Table of Contents|

脂肪干细胞诱导成心肌细胞研究(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2005年第5期
页码:
405-408
栏目:
基础研究
出版日期:
2005-10-05

文章信息/Info

Title:
Transformation of adiposederived stem cells into cardiomyocytes
作者:
张端珍1; 盖鲁粤2; 刘宏伟2; 朱鲜阳1
1.沈阳军区总医院先心病内科，辽宁 沈阳 110016；2.解放军总医院心内科，北京 100853
Author(s):
ZHANG Duanzhen1;  GAI Luyue2;  LIU Hongwei2;  ZHU Xianyang1
1.Department of Cardiology, Shenyang Military General Hospital, Shenyang, Liaoning 110016, 2.Department of Cardiology, Chinese PLA General Hospital, Beijing 100853, China
关键词:
脂肪; 干细胞; 心肌细胞
Keywords:
adipose;  stem cells;  cardiomyocyte
分类号:
Q254;R329.28
DOI:
-
文献标识码:
A
摘要:
目的 探讨体外培养的脂肪干细胞（ADSCs）转化为心肌细胞的可能性和最佳诱导时机。方法 取新西兰白兔ADSCs，体外培养扩增。将传代不同次数的ADSCs以不同的5氮杂胞苷浓度诱导，探讨最佳诱导浓度和时机，观察诱导后细胞的形态变化以及传代对心肌管样细胞数量的影响。免疫细胞化学方法鉴定。结果 ADSCs接种后生长迅速，呈短梭形，细胞形态单一。5氮杂胞苷浓度对细胞的生长有显著影响，随着5氮杂胞苷浓度的增加，细胞数量显著减少。诱导剂有效浓度为6～9 μmol/L，以终浓度为9 μmol/L对第3代ADSCs诱导产生的心肌细胞数量最多。5氮杂胞苷诱导后最早于第9天形成肌管样结构，以肌纤维蛋白、α－横纹肌肌动蛋白和心肌肌钙蛋白T单克隆抗体检测均为阳性。诱导后细胞传代导致心肌细胞数量显著减少。结论 ADSCs在体外可有效转化为心肌细胞，它可能是心肌细胞移植的良好细胞来源。
Abstract:
AIM To investigate whether adiposederived stem cells（ADSCs） could differentiate into cardiomyocytes in vitro. METHODS Adiposederived stem cells were isolated from the fatty tissue of New Zealand White rabbits and cultured in IMDM medium. Various passaged ADSCs were treated with various concentrations of 5azacytidine and incubated for 24 hours. The transformed cells were subjected to immunostaining for the desmin,αsarcromeric actin, and cardiac troponinT. RESULTS Cultured ADSCs displayed a uniform fibroblastlike morphology. The concentration of 5azacytidine had great effects on the cell growth. With the addition of 5azacytidine, the cells were transformed into cardiomyocytes. At 2 weeks, the cells expanded and took on a ballshaped appearance. Immunostaining of the transformed cells for desmin, αsarcromeric actin, and cardiac troponinT was positive. 5azacytine at final concentration of 6-9 μmol/L worked well on cell transformation and the optimistic final concentration of 5azacytidine was 9 μmol/L. CONCLUSION Adiposederived stem cells can be chemically transformed into cardiomyocytes，which would be a useful source of cardiomyocyte progenitors.

参考文献/References

［1］ Anversa P, NadalGinard B. Myocyte renewal and ventricular remodeling［J］. Nature，2002，415：240-243.

［2］ Tholpady SS, Katz AJ, Ogle RC. Mesenchymal stem cells from rat visceral fat exhibit multipotential differentiation in vitro［J］. Anat Rec，2003，272A：398-402.

［3］ Planat-Benard V,Silverstre JS, Cousin B, et al. Plasticity of human adipose lineage cells toward endothelial cells: physiological and therapeutic perspectives［J］. Circulation，2004，109：656-663.

［4］ PlanatBenard V, Menard C, Andre M, et al. Spontaneous cardiomyocyte differentiation from adipose tissue stroma cells［J］. Circ Res，2004,94：223-229.

［5］ Constantinides PG, Jones PA, Gevers W. Functional striated muscle cells from nonmyoblast precursors following 5azacytidine treatment［J］. Nature，1977，267：364-366.

［6］ Wakitani S, Saito T, Caplan AI. Myogenic cells derived from rat bone marrow mesenchymal stem cells exposed to 5azacytidine［J］. Muscle and Nerve，1995，18：1417-1426.

［7］ De Ugarte DA, Morizono K, Elbarbary A，et al. Comparison of multilineage cells from human adipose tissue and bone marrow［J］. Cells Tissues Organs，2003，174：101-109.

［8］ Rangappa A，Fen C，Lee EH，et al. Transformation of adult mesenchymal stem cells isolated from the fatty tissue into cardiomyocytes［J］. Ann Thorac Surg，2003，75：775-779.

［9］ Gaustada KG, Boquesta AC, Andersonb BE, et al. Differentiation of human adipose tissue stem cells using extracts of rat cardiomyocytes［J］. Biochem Biophys Res Com，2004，314：420-427.

备注/Memo

备注/Memo:
收稿日期：2004-10-09.
更新日期/Last Update: 2010-01-05