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|本期目录/Table of Contents|

一种心肌细胞替换实验技术的研究(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2005年第5期
页码:
445-446
栏目:
基础研究
出版日期:
2005-10-05

文章信息/Info

Title:
An experimental technique for cellular cardiomyoplasty
作者:
文峻1; 刘鲁伟2; 范云1; 屈学民1; 龙开平1; 王斯刚1
第四军医大学：1．物理学教研室，2．门诊部， 陕西 西安 710032
Author(s):
WEN Jun1;  LIU Luwei2;  FAN Yun1;  QU Xuemin1;  LONG Kaiping1;  WANG Sigang1
1.Department of Physics, 2.Outpatient department, Fourth Military Medical University, Xi′an, Shaanxi 710033，China
关键词:
小鼠; 外科; 实验; 技术; 心肌细胞
Keywords:
mouse; surgery; experiment; technique; cardiomyocytes
分类号:
Q813
DOI:
-
文献标识码:
A
摘要:
目的 研究一种新的能将细胞注入到小鼠跳动心脏的受伤部位，以治疗受损心脏的实验外科技术。方法 在动物外科手术中设计一种新的固定装置以提供细胞注入通道，并采用一个转基因小鼠模型提供特殊心肌细胞。结果 与对照组相比，小鼠存活率显著提高（P<0.01）。结论 利用绿色荧光蛋白（GFP）标记的胚胎心肌细胞对受损心脏的修复可使细胞替换疗法的实验研究变得更加方便。
Abstract:
AIM To explore a new experimental surgical technique for the treatment of heart failure. METHODS A newly designed apparatus for stabilizing a beating mouse heart was applied with a builtin channel through which prepared cell suspension was injected into the cryoinjured area of left ventricles. A transgenic mouse model was used to provide the special cells for the injection. RESULTS The number of green luorescent protein(GFP)labeled cardiomyocytes of the injured myocardium using the new technique was significantly higher than using syringes for cell injection(P<0.01). CONCLUSION The new technique significantly increases ostoperative survival rate.

参考文献/References

［1］ Reinlib L, Field LJ. Cell transplantation as future therapy for cardiovascular disease［J］. Circulation, 2000, 101: E182- E187.

［2］ Klug MG, Soonpaa MH, Koh GY, et al. Genetically selected cardiomyocytes from differentiating embronic stem cells form stable intracardiac grafts［J］. J Clin Invest, 1996, 98: 216-223.

［3］ Kolossov E, Fleischmann BK, Liu Q, et al. Functional characteristics of ES celLderived cardiac precursor cells identified by tissue-specific expression of the green fluorescent protein［J］. J Cell Biol, 1998, 143: 2045-2051.

［4］ Reinecke H, MacDonald GH, Hauschka SD, et al. Electromechanical coupling between skeletal and cardiac muscle: Implications for infarct repair［J］. J Cell Biol, 2000, 149: 731-734.

［5］ Taylor DA, Atkins BZ, Hungspreugs P, et al. Regenerating functional myocardium: improved performance after skeletal myoblast transplantation［J］. Nat Med, 1998, 4: 929-934.

［6］ Sakai T, Li RK, Weisel RD, et al. Fetal cell transplantation: A comparison of three cell types［J］. J Thorac Cardiovasc Surg, 1999, 118: 715-722.

备注/Memo

备注/Memo:
收稿日期：2004-12-20.
更新日期/Last Update: 2010-01-05