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同型半胱氨酸对血管内皮细胞的氧化应激损伤及对IL8分泌的影响(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2005年第5期
页码:
450-452
栏目:
临床研究
出版日期:
2005-10-05

文章信息/Info

Title:
Homocysteine induces oxidative stress injury and IL8 secretion in vascular endothelial cells in vitro
作者:
张铭;  陈怀生;  杨大春;  曾智;  余敏
四川大学华西医院心内科，四川 成都 610041
Author(s):
ZHANG Ming; CHEN Huaisheng;  YANG Dachun; ZHENG Zhi;  YU Min
Department of Cardiology,West Hosipital,Sichuan University,Chengdu,Sichuan 610041,China
关键词:
同型半胱氨酸; 氧化应激损伤; 动脉粥样硬化; 白细胞介素8
Keywords:
homocysteine; oxidative stress impair;  atherosclerosis; interleukin8
分类号:
R543.12
DOI:
-
文献标识码:
A
摘要:
目的 通过观察病理浓度下的同型半胱氨酸HCY对培养的内皮细胞（EC）损伤及其氧化应激的影响。同时检测IL8的表达，探讨HCY在动脉粥样硬化（AS）中的作用。方法 体外培养人脐静脉血管EC ECV304。测定氧化应激损伤指标，分正常对照组和HCY组（浓度分别为50、100 μmol/L、1.0、2.5、5.0 mmol/L组）。测细胞因子IL8的分泌表达，分正常对照组和HCY组（浓度分别为50、200 μmol/L组），孵育0.5、8、24 h后,测IL8表达。结果 高浓度HCY对EC损伤明显，IDH值升高，活性氧和MDA水平增高，而SOD，CAT活性下降，NO的生成减少。IL8的表达量增加，且分泌高峰提前出现。结论 病理水平HCY可以增强氧化应激损伤反应，刺激EC分泌IL8。
Abstract:
AIM To observe the effect of pathological level of homocysteine(HCY) on cells and oxidative stress and the secretion of IL8 in endothelium. METHODS 1.ECV304 human umbilical endothelial cells were incubated in vitro and treated with HCY at diffent concentrations. The levels of CAT,NO,SOD,MDA were determined. The expression of IL8 was assessed by enzymelinked immunosorbent assay. RESULTS ① High HCY caused cell injuries, increased LDH,activate oxygen and MDA levels. while the levels of SOD,CAT and NO were reduced. ②High HCY induced the expression of IL8. CONCLUSION Pathologicial level of HCY could impair endothelial cells, induce oxidative stress injury and IL8 secretion of endothelial cells.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2003-10-10.
更新日期/Last Update: 2010-01-05