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几丁糖抗实验性家兔动脉粥样硬化的作用(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2005年第6期
页码:
521-523
栏目:
基础研究
出版日期:
2005-12-05

文章信息/Info

Title:
Effects of chitosan on experimental rabbit atherosclerosis
作者:
顾兴建; 吴宗贵; 任雨笙; 殷仁富; 黄高忠
第二军医大学长征医院心内科，上海 200003
Author(s):
GU Xingjian;  WU Zonggui;  REN Yusheng;  YIN Renfu;  HUANG Gaozhong
Department of Cardiology, Changzheng Hospital, Second Military Medical University, Shanghai 200003,China
关键词:
几丁糖; 动脉粥样硬化; PDGFRβ; 兔
Keywords:
chitosan;  atherosclerosis;  PDGFRβ; rabbit
分类号:
R543.5
DOI:
-
文献标识码:
A
摘要:
目的 研究几丁糖对实验性家兔动脉粥样硬化的影响及其机制。方法 采用高脂喂养的家兔动脉粥样硬化模型，实验分为普食组、高脂组、几丁糖高脂组、几丁糖普食组，第0、4、8周空腹采血测定血脂，第8周处死动物，制备主动脉标本，油红O染色测定降主动脉粥样硬化斑块面积，升主动脉HE染色和血小板衍生生长因子受体β（PDGFRβ）免疫组化染色。结果 第4、8周几丁糖高脂组甘油三酯（TG）、总胆固醇（TC）、低密度脂蛋白胆固醇（LDLC）均低于高脂组，普食组与几丁糖普食组血脂水平相似；几丁糖高脂组的斑块面积显著小于高脂组；HE染色示几丁糖高脂组内膜增厚轻于高脂组；几丁糖高脂组内膜、中膜PDGFRβ表达较高脂组减弱。结论 几丁糖具有抗动脉粥样硬化作用，与其降低血脂和抑制血管的PDGFRβ表达有关。
Abstract:
AIM To investigate the effects of chitosan on experimental rabbit atherosclerosis (AS). METHODS The rabbit models of AS were produced by a high fat diet. The rabbits were randomly assigned into normal diet（ND） group, high fat diet（HFD）group, chitosan and high fat diet（CHFD） group, chitosan and normal diet（CND）group. Fasting blood samples were collected for serum lipid assay at 0, 4, 8 wk. The animals were sacrificed at 8 wk to make aorta samples. AS plaques areas were estimated in the descending aorta dyed with oil red O and the ascending aorta were dyed with HE and PDGFRβ immunohstochemistry. RESULTS TG, TC, LDLC in the CHFD group were lower than those in the HFD group at 4, 8 wk, while serum lipid concentration in the ND group was similar with that in the CND group. Compared with HFD group, plaques areas were smaller, intima was thinner and PDGFRβ in the intima and media was less expressed in the CHFD group. CONCLUSION Chitosan reduces AS degree in rabbits fed on diet rich in fat, which might be related to the decrease of serum lipid and the downrelugation of PDGFRβ expression in the artery.

参考文献/References

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［4］ 余艳辉，瞿湘萍，李智，等. 兔高脂蛋白血症模型建立及动脉粥样斑块形成的方法学［J］. 中国药理学通报，1998，14（5）：473-474.

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备注/Memo

备注/Memo:
收稿日期：2004-12-28.作者简介：顾兴建，主治医师，博士Tel：(021)6361010973202
更新日期/Last Update: 2010-01-06