前列腺素E1对犬心肺联合移植供肺的保护作用(PDF)
《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]
- 期数:
- 2006年第2期
- 页码:
- 165-167，177
- 栏目:
- 基础研究
- 出版日期:
- 2006-03-01
文章信息/Info
- Title:
- Protective effect of PGE1 on donor lung in canine heart-lung transplantation
- Author(s):
- ZHONG Jia-guo; YI Ding-hua; YU Shi-qiang; LIU Jin-cheng; ZHANG Sheng-li; CHENG Liang
- Department of Cardiovascular Surgery, Institute of Cardiology, Xijing Hospital, Fourth Military Medical University, Xi′an ，Shaanxi 710032, China
- Keywords:
- heart-lung transplantation; prostaglandin E1 ; lung preservation
- 分类号:
- R617
- DOI:
- -
- 文献标识码:
- A
- 摘要:
- 目的 研究前列腺素E1(PGE1)在犬心肺联合移植过程中对供肺的保护作用。方法 建立犬心肺联合移植模型，先于供体肺动脉内注射PGE1，再在肺动脉内灌注含有PGE1的低温EC（Euro-Collins）溶液，恢复血流灌注后观察实验组及对照组在30、60、90、120 min的平均肺动脉压（MPAP）、动脉血氧分压(PaO2)的变化，恢复血流灌注1 h后，取移植肺组织为标本，检测肺组织中髓过氧化物酶(MPO)活力、肺湿干质量比，HE染色,光镜下观察病理变化。结果 实验组中PaO2值在各时间段较对照组明显升高，MPAP值在各时间段较对照组明显降低。实验组肺组织中MPO活性显著低于对照组，实验组的肺湿干质量比较对照组显著降低。病理切片显示对照组的肺组织有较严重的白细胞浸润及肺水肿形成，而实验组的病变程度较对照组轻微。结论 在心肺联合移植过程中应用前列腺素E1，可减轻移植肺缺血/再灌注损伤，保护移植肺的肺功能，增加心肺联合移植的成功率。
- Abstract:
- AIM To investigate donor lung preservation by using PGE1 in canine heartlung transplantation. METHODS PGE1 administered before regurgitation in the caniae model of heart-lung transplantation. The changes of Pa02 of arterial blood and MPAP in two groups at 30, 60, 90 and 120 min were observed. The lung tissue samples were obtained one hour after reperfusion. The wet/dry ratio of lung tissue myeloperoxidase (MPO) activity were measured. Microscopic examination of lungs was also conducted. RESULTS In experimental group, arterial blood Pa02 respectively at 30, 60, 90 and 120 min was significantly higher than that in control group while MPAP in experimental group respectively at 30, 60, 90 and 120 min was significantly lower than that in control group. The wet/dry ratio of lung and the tissue MPO activity in experimental group were significantly lower than those in control group. The microscopic examination showed that there was severe leukocyte infiltration and edema formation in the alveolar space in control group but the changes were less severe in experimental group. CONCLUSION Administration of PGE1 in canine heartlung transplantation can protect the lung against ischemia/reperfusion injury, improve the allografted lung function and increase the successful rate of heartlung transplantation.
参考文献/References
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［3］ Lin PJ，Hsieh MJ，Chen KS, et al．University of Wisconsin solution extends lung preservation after prostaglandin El infusion［J］．Chest，1994，105(1)：255-261.
［4］ Sayan H，Ugurlu B，Babul A，et al．Effects of Larginine and NGnitro Larginine methyl ester on lipid peroxide，superoxide dismutase and nitrate levels after experimental sciatic nerve ischemiareperfusion in rats［J］．Int J Neurosci，2004，ll4(3)：349-364.
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备注/Memo
- 备注/Memo:
- 收稿日期：2005-09-20.通讯作者：易定华，主任医师，教授，主要从事复杂先心病、心脏瓣膜病以及心脏移植方面的研究 Tel:(029)84775307Email: yidh@fmmu.edu.cn 作者简介：钟家国，硕士生Tel:（029）84775311 Email: royzhong@fmmu.edu.cn
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