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风湿性心脏病患者血清抗β1肾上腺素受体自身抗体的生物学效应(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2006年第2期
页码:
194-197
栏目:
临床研究
出版日期:
2006-03-01

文章信息/Info

Title:
Biological effects of autoantibody against β1adrenoceptor in patients with rheumatic valvular heart diseases
作者:
贺忠梅1; 李美霞1; 王晓樑1; 王晋萍2; 汤嘉宁3; 魏书庆3; 刘慧荣1
山西医科大学：1. 生理学教研室，2. 山西省心血管病研究所，3. 附属太钢总院，山西 太原 030001
Author(s):
HE Zhong-mei1;  LI Mei-xia1;  WANG Xiao-liang1;  WANG Jin-ping2;  TANG Jia-ning3;  WEI Shu-qing3;  LIU Hui-rong1
1. Department of Physiology, 2.Shanxi Provincial Heartvein Hospital, 3. General Hospital of Taiyuan Iron and Steel Company, Shanxi Medical University, Taiyuan, Shanxi 030001, China
关键词:
风湿性心脏病; β1肾上腺素能受体; 自身抗体; 生物效应
Keywords:
rheumatic valvular heart disease;  β1adrenoceptor;  autoantibody;  biological activities.
分类号:
R541.2
DOI:
-
文献标识码:
A
摘要:
目的 探讨风心病患者血清中抗β1肾上腺素能受体（β1AR）自身抗体的生物学效应。方法 ① 应用SAELISA方法，检测风心病患者（60例），正常人（97例），以及心衰大鼠（33只）和对照大鼠（20只）血清标本中的抗β1AR自身抗体；②亲和层析法纯化风心病患者、心衰大鼠抗体阳性血清中的IgGs； ③ 观察抗β1AR自身抗体IgGs对乳鼠心肌细胞搏动频率的影响；④测定抗β1AR自身抗体IgGs 对成年大鼠心肌细胞腺苷酸环化酶活性的影响。结果 风心病患者血清中抗β1AR自身抗体的阳性率和抗体滴度分别为43%和1∶(98.3±1.6)，均显著高于正常人的4%和1∶( 14.6±2.7)，P<0.01，类似的结果见于心衰大鼠［85%，1∶( 67.3±2.4)］与对照大鼠［5%, 1∶(17.3±2.1)］(P<0.01)之间；纯化的风心病患者、心衰大鼠抗β1AR抗体IgGs 均可增加乳鼠心肌细胞的搏动频率，且在6 h内不脱敏；还可增强成年大鼠心肌细胞的腺苷酸环化酶活性，使cAMP产生量增加。 结论 风心病患者血清中的抗β1AR自身抗体具有激动剂样刺激效应，即具有正性变时效应（不易脱敏）、可增强腺苷酸环化酶活性。提示抗β1AR自身抗体可能是风心病患者神经体液调节功能紊乱的原因之一。
Abstract:
AIM To determine the biological effects of autoantibody against β1adrenoceptor in patients with rheumatic valvular heart diseases (RVHD). METHEDS ①Synthetic peptides corresponding to amino acids of the second extracellular loops of the β1adrenoceptor used as the antigen to screen the sera from RVHD patients (n=60), normal control (NC, n=97), rats with heart failure (n=33) and control rats (n=20) by using SAELISA. ② IgGs from the positive sera of the patients with RVHD and rats with heart failure were prepared by using a MabTrap Kit (Amersham) following the manufacturer’s instructions. ③ The effects of IgGs from the positive sera on the beating rate of cultured neonatal rat cardiomyocytes were observed. ④ By using radioimmunoassay technique, the activity of adenylyl cyclase (AC) in adult rat cardiomyocytes was observed. RESULTS The positive rate and titer of antiβ1adrenoceptor autoantibody from the patients with RVHD were obviously higher than those from normal control ［43%, 1∶(98.3±1.6)］ vs ［4%, 1∶(14.6±2.7)］,( P<0.01) and similar result was found in rats with heart failure and control rats ［85%, 1∶(67.3±2.4)］ vs ［5%, 1∶(17.3±2.1)］,( P<0.01). The IgGs from patients with RVHD and rats with heart failure not only increased the beating rate of cultured neonatal rat cardiomyocytes and nondesensitization in 6 hours, but also enhanced the activity of AC in adult rat cardiomyocytes and increased the cyclic adenosine monophosphate (cAMP) content. CONCLUSION Like β1adrenoceptor agonists, the autoantibody against β1adrenoceptor in patients with RVHD induces positive chronotropic effects, indicating that the autoantibody against β1adrenoceptor functions as an agonist and participates in the pathophysiological mechanisms of RVHD.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2005-05-24.基金项目：山西省自然科学基金项目资助（No.20021103）；山西省教育厅普通教育基金项目资助（No.200476）通讯作者：刘慧荣，教授 Tel：(0351)4135076 Email:liuhr2000@hotmail.com 作者简介：贺忠梅，讲师，硕士 Tel:（0351）4135076 Email:hezmei@126.com
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