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|本期目录/Table of Contents|

CD40L刺激诱导型环氧合酶表达及普伐他汀的作用(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2006年第3期
页码:
245-248
栏目:
基础研究
出版日期:
2006-06-25

文章信息/Info

Title:
Effect of pravastatin on cyclooxygenase-2 expression induced by rhCD40L in cultured U937 cells
作者:
樊民1; 吴宗贵1; 李岚2; 李洪涛1; 王磊1; 潘晓明1
1.第二军医大学长征医院心内科,上海 200003; 2. 上海411医院内四科,上海 200003
Author(s):
FAN Min;  WU Zong-gui;  LI Lan;  Li Hong-tao;  Wang Lei;  Pan Xiao-ming
Department of Cardiovasology, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China
关键词:
CD40配体; 诱导型环氧合酶; 普伐他汀; 单核细胞
Keywords:
CD40 ligand; cyclooxygenase-2; pravastatin; monocyte
分类号:
Q946.5; R972.9
DOI:
-
文献标识码:
A
摘要:
目的 探讨重组人CD40配体（rhCD40L）对培养的人单核细胞（U937）表达诱导型环氧合酶（COX-2）的作用和普伐他汀对其影响。方法 体外培养U937细胞，以0.05、0.1、0.2 μg/ml的rhCD40L分别刺激24 h；0.4 μg/ml的rhCD40L分别刺激3、6、12、24 h；终浓度10、1、0.1 mmol/L的普伐他汀与0.4μg/ml的rhCD40L共同刺激U937细胞24 h。RT-PCR检测COX-2 mRNA表达，Westernblot 检测COX-2蛋白表达，ELISA法检测前列腺素E2（PGE2）的浓度反映COX-2酶活性。结果 0.4 μg/ml的rhCD40L刺激后3 h可诱导COX-2 mRNA和蛋白表达，随着rhCD40L浓度和作用时间增加，COX-2 mRNA和蛋白表达显著增加（P<0.01），且呈时效和量效关系；同样，rhCD40L以时间和剂量依赖的方式增加U937细胞中PGE2的合成。0.4 μg/ml的rhCD40L与普伐他汀共同刺激24 h后，COX-2的表达显著被抑制。结论 rhCD40L以时间和浓度依赖的方式诱导COX-2的表达，普伐他汀可以抑制此作用。
Abstract:
AIM To evaluate cyclooxygenase2 expression induced by rhCD40L in cultured U937 cells and possible inhabition inhibition by pravastatin. METHODS U937 cells were pretreated with rhCD40L of different concentrations and time intervals and COX2 mRNA and protein expression were observed by RTPCR and Westernblot respectively. For COX2 enzyme activity, PGE2 released by U937 cells was measured by ELISA. RESULTS rhCD40L increased the expression of COX2 in a time and dosedependent manner (P<0.01). Pravastatin inhibited the expression of COX2 induced by rhCD40L in a dosedependent manner (P<0.01). CONCLUSION rhCD40L can induce cyclooxygenase2 expression in a dose and time dependent manner. Pravastatin inhibits this enhanced expression in U937 cells.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2005-09-16.通讯作者：吴宗贵，主任医师，主要从事炎症与动脉粥样硬化研究 作者简介：樊民，主治医师，博士 Tel：（021）6361010973202 Email：fanmin@medmail.com.cn
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