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|本期目录/Table of Contents|

自发性高血压大鼠心脏成纤维细胞生物活性的变化(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2006年第3期
页码:
277-279
栏目:
基础研究
出版日期:
2006-06-25

文章信息/Info

Title:
Changes of activities of fibroblasts derived from spontaneously hypertensive rat
作者:
张海涛1; 赵连友2; 刘朝中1; 王荫静3; 罗慧兰1; 于心亚1; 田建伟2
1.空军总医院心内科，北京100036；2.第四军医大学唐都医院心内科，陕西 西安 710038；3.空军总医院临床试验中心
Author(s):
ZHANG Hai-tao;  ZHAO Lian-you;  LIU Chao-zhong;  WANG Yin-jing; LUO Hui-lan;  YU Xin-ya;  TIAN Jian-wei
Department of Cardiology， General Hospital of Air Force, PLA, Beijing 100036
关键词:
成纤维细胞; 左心室肥厚; 增殖; 胶原合成; 一氧化氮
Keywords:
fibroblasts;  left ventricular hypertrophy;  proliferation;  collagen synthesis;  nitric oxide
分类号:
Q251;R544.1
DOI:
-
文献标识码:
A
摘要:
目的 观察基础状态下成年高血压大鼠(SHR)心肌成纤维细胞（CFs)的吸光值、增殖细胞核抗原（PCNA）表达、3H胸腺嘧啶核苷（3HTdR）及3H脯氨酸掺入法（3HＰroline）掺入量和NO含量的变化。方法胰酶消化法分离、培养大鼠CFs，分别用改良的MTT比色法、免疫组化法、3HTdR掺入法观察CFs的增殖状况，用3H-Ｐroline观察CFs的胶原合成情况，用硝酸还原酶法检测CFs培养液中的NO含量。结果 培养72 h，SHR组与正常血压组比较， CFs吸光值、PCNA表达、3H-TdR及3H-Ｐroline掺入量增高具有非常显著意义 (P<0.01)，NO含量降低也具有非常显著意义 (P<0.01)。结论 CFs在维持基质间隙中起着关键作用，其增殖、胶原合成以及NO含量的异常都可以导致左心室肥厚的产生和发展。
Abstract:
AIM To evaluate the changes of MTT OD value、PCNA protein expression、3HTdR and 3Hproline incorporation and NO content in rat cardiac fibroblasts(CFs) derived from SHR. METHODS CFs were isolated by trypsin digestion method. Modified MTT array was used to determine cell number. 3HTdR incorporation was used to analyze PCNA protein expression and determine DNA synthesis. Collagen synthesis and NO content were also detected. RESULTS Under basal condition (72 h),the A value, PCNA protein expression,3HTdR and 3Hproline incorporation derived from SHR were significantly greater than those of Wistar rats (P<0.01), while NO content derived from SHR were significantly lower than that of Wistar rats (P<0.01). CONCLUSION The abnormal changes of CFs, collagen content of CFs and NO content in SHR might be related to the formation and development of left ventricular hypertrophy.

参考文献/References

［1］ 杨学东,赵连友,李雪,等. 精氨酸加压素诱导大鼠心脏成纤维细胞胶原合成的作用［J］. 心脏杂志,2000，12(3):169-171.

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［3］ Liu Y，Kitsis RN. Induction of DNA synthesis and apoptosis in cardiac myocytes by EIA on protein［J］. J Cell Biol,1996， 133(2):325-334.

［4］ 张海涛, 赵连友,王荫静,等.卡维地洛对高血压大鼠心脏成纤维细胞胶原合成的抑制作用［J］. 心脏杂志,2003，15(3):205-208.

［5］ Cao L,Gardner DG.Natriuretic peptides inhibit DNA synthesis in cardiac fibroblasts［J］. Hypertension,1995,25(2 ):227-234.

［6］ Farivar RS,Chobanian AV,Brecher P. Salicylate or aspirin inhibits the induction of the inducible nitric oxide synthase in rat cardiac fibroblasts［J］. Circ Res, 1996，78(5): 759-768.

［7］ Hou J, Kato H, Cohen RA, et al. Angiotensin IIinduced cardiac fibrosis in rat is increased by chronic inhibition of nitric oxide synthase［J］. J Clin Invest, 1995, 96(5):2469-2477.

备注/Memo

备注/Memo:
收稿日期: 2005-04-12.作者简介：张海涛，主治医师，硕士 Tel：（010）80682773 Email：kjzht@sina.com
更新日期/Last Update: