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动脉粥样硬化中IL-4与体内15-LO基因启动子区甲基化的关系(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2006年第4期
页码:
373-375,391
栏目:
基础研究
出版日期:
2006-08-25

文章信息/Info

Title:
Plasma interleukin-4 concentration is related with methylation status of 15-lipoxygenase promoter in atherosclerosis
作者:
姜文瑞1; 舒青2; 谢学建3; 郑强荪1
第四军医大学:1.唐都医院心内科，陕西 西安，710038，2．基础部遗传学与发育生物学教研室，陕西 西安，710032，3．西京医院心内科，陕西 西安 710032
Author(s):
JIANG Wen-rui1;  SHU Qing2;  XIE Xue-jian3;  ZHENG Qiang-sun1
1.Department of Cardiology, Tangdu Hospital, Xi′an,Shaanxi 710038, China, 2.Department of Medical Genetics and Developmental Biology, School of Basic Medicine, Xi′an,Shaanxi 710032 China, 3.Department of Cardiology, Xijing Hospital, Fourth Military Medical University, Xi′an, Shaanxi 710032
关键词:
动脉粥样硬化; 15-脂氧合酶; DNA甲基化; 白细胞介素-4
Keywords:
atherosclerosis;  15-lipoxygenase;  DNA methylation;  interleukin-4
分类号:
R541.4
DOI:
-
文献标识码:
A
摘要:
目的 探讨在动脉粥样硬化中血清白介素-4（IL-4）浓度是否与15-脂氧合酶（15-LO）基因启动子区甲基化状态有关。方法 连续采集51例2004年12月至2005年6月在西京医院和唐都医院行冠状动脉造影术的男性成人血样，用改进的半定量甲基化特异性PCR检测其体内15-LO基因启动子区甲基化状态，计算甲基化特异性条带和非甲基化特异性条带吸光值（A）比值；夹心法ELISA检测其血清IL-4浓度。结果 ①按照A比值把全部实验对象分为低甲基化组（比值＜1,26例）和高甲基化组（比值≥1，25例），低甲基化组血清IL-4平均浓度［（89±27）ng/L］及动脉粥样硬化患病率（77％）显著高于高甲基化组［（50±34)ng/L，40％，均P<0.01］。②在动脉粥样硬化组和正常对照组内部，低甲基化组IL-4平均浓度［（87±20）ng/L和（96±17）ng/L］显著高于高甲基化组［（48±35）ng/L和（52±34）ng/L，均P<0.01］。结论 血清IL-4浓度与外周血15-LO基因启动子区甲基化状态相关，在动脉粥样硬化发病过程中发挥重要作用。
Abstract:
AIM To explore whether plasma IL-4 concentration is related with the status of 15-lipoxygenase gene promoter methylation in atherosclerosis. METHODS Twentyfive cases were included in the hypermethylated group and 26 cases were in the hypomethylated group. The atherosclerosis group (30 cases) and healthy group (21 cases) were subdivided respectively into hypomethylated group and hypermethylated group. Semiquantitative methylation specific PCR was used to analyze the methylation status of 15lipoxygenase promoter and ELISA was used to detect the plasma IL4 concentration. RESULTS ①Average IL-4 concentration in the hypomethylated group （89±27)ng/L was significantly higher than that in the hypermethylated group（50±34)ng/L and IL-4 concentration in each hypomethylated group（87±20)ng/L and (96±17)ng/L was also significantly higher than that in each hypermethylated group（48±35) ng/L and (52±34)ng/L. ②The incidence of atherosclerosis in hypomethylated group（77％）was significantly higher than that in the healthy group（40％）. CONCLUSION Plasma interleukin-4 concentration is related with methylation status of 15lipoxygenase promoter and may play an important role in the pathogenesis of atherosclerosis.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2006-02-17.基金项目:国家自然科学基金项目资助（No.39700165） 通讯作者:郑强荪，主任医师，主要从事冠心病的发病机制研究 Tel:(029)84777422 Email: QINGSHU@fmmu.edu.cn 作者简介:姜文瑞，硕士生 Tel:(029)8477722Email: tuosituo@fmmu.edu.cn
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