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雌激素对PDGF诱导的血管平滑肌细胞增殖及细胞周期的影响(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2006年第6期
页码:
611-613
栏目:
基础研究
出版日期:
2006-12-25

文章信息/Info

Title:
Effect of estrogen on proliferation and cell cycle of cultured rabbit vascular smooth muscle cells induced by platelet-derived growth factor
作者:
周永兰1陈清枝2张延斌2王人彭1张义勤1骆秉辁1
1.江苏大学附属医院徐州第三人民医院心内科,江苏 徐州 221005;2.徐州医学院附属医院心内科,江苏 徐州 221002
Author(s):
ZHOU Yong-lan1 CHEN Qing-zhi2 ZHANG Yan-bin2 WANG Ren-peng1 ZHANG Yi-qin1 LUO Bing-quan1
1.Department of Cardiology, Xuzhou Third People′s Hospital, Affiliated Xuzhou Hospital of Jiangsu University, Xuzhou,Jiangsu 221005; 2. Department of Cardiology, Affiliated Hospital of Xuzhou Medical University, Xuzhou,Jiangsu 221002, China
关键词:
雌二醇血管平滑肌细胞增殖细胞周期血小板源生长因子
Keywords:
estradiolvascular smooth muscle cells proliferation cell cycle platelet-derived growth factor
分类号:
R458.7
DOI:
-
文献标识码:
A
摘要:
目的 探讨17β-雌二醇(E2)对血管平滑肌细胞(VSMC)增殖及细胞周期的影响。方法 分离培养VSMC,血小板源生长因子(PDGF)诱导其增殖,应用MTT法及流式细胞仪检测不同浓度E2(10和100 nmol/L)对传代VSMC增殖活性及细胞周期的影响。结果 E2 (10和100 nmol/ L) 作用下VSMC增殖活性下降, 且与浓度有关;对VSMC细胞周期的分布影响主要表现为处于G0/G1期细胞数增多,G2/S期的细胞数减少(P<0.01)。结论 E2具有抑制VSMC增殖的作用,其部分机制可能是通过阻滞VSMC G0/G1期向S的转化有关。
Abstract:
AIM To investigate the effect of 17β estradiol ( E2) on the proliferation of vascular smooth muscle cells (VSMC) and the possible underlying mechanisms. METHODS A cell proliferating model of rabbit aorta VSMC induced by PDGFBB was established. The proliferation was determined with MTT metabolism in subcultured VSMC exposed to different concentrations (10 and 100 nmol/L) of 17β estradiol and the cell cycle was examined by flow cytometry. RESULTS MTT metabolism of VSMCs induced by PDGFBB was inhibited by E2 and the cells numbers of G0/G1 phase increased and the cells numbers of G2/S phase decreased markedly (P<0.01). CONCLUSION 17β estradiol inhibits the proliferation of VSMC induced by PDGFBB, at least in part by preventing VSMCs entering S phase from G0/G1 phase.

参考文献/References

[1] Ling S,Dai A, Dilley RJ,et al.Endogenous estrogen deficiency reduces proliferation and enhances apoptosisrelated death in vascular smooth muscle cells: insights from the aromataseknockout mouse[J]. Circulation,2004,109(4): 537-543.

[2] Yue TL,VickeryClark L,Louden CS,et al.Selective estrogen receptor modulator idoxifene inhibits smooth muscle cell proliferation, enhances reendothelialization,and inhibits neointimal formation In vivo after vascular injury[J]. Circulation,2000,102(19 suppl 3):III281-III288.

[3] Li J,Huang SL,Guo ZG.Plateletderived growth factor stimulated vascular smooth muscle cell proliferation and its molecular mechanism[J]. Acta Pharmacol Sin,2000,21(4): 340-344.

[4] Worboys S,Kotsopoulos D, Teede H,et al.Evidence that parenteral testosterone therapy may improve endotheliumdependent and independent vasodilation in postmenopausal women already receiving estrogen[J]. Clin Endocrinol Metab,2001,86(1): 158-161.

[5] Sudoh N, Toba K, Akishita M,et al.Estrogen prevents oxidative stress–induced endothelial cell apoptosis in rat[J]. Circulation,2001,103(5):724-729.

[6] Strehlow K, Rotter S, Wassmann S,et al.Modulation of antioxidant enzyme expression and function by estrogen [J]. Circ Res,2003,93(2): 170-177.

[7] Liu HW,Iwai M,TakedaMatsubara Y,et al.Effect of estrogen and AT1 receptor blocker on neointima formation[J]. Hypertension,2002,40(4): 451-457.

[8] Hodgin JB,Knowles JW, Kim HS,et al.Interactions between endothelial nitric oxide synthase and sex hormones in vascular protection in mice[J]. Clin Invest,2002,109(4): 541-548.

备注/Memo

备注/Memo:
收稿日期:2006-01-16.作者简介:周永兰,主治医师,硕士生 Tel:(0516)83770891 Email:zhouyonglan94101@yahoo.com.cn
更新日期/Last Update: