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银杏叶提取物对活性氧诱导的血管平滑肌细胞增殖的影响(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2006年第6期
页码:
629-631
栏目:
基础研究
出版日期:
2006-12-25

文章信息/Info

Title:
Effects of Gingko biloba extract on smooth muscle cell proliferation induced by reactive oxygen species
作者:
胡涛1; 贾国良1; 李寰1; 王海昌1; 郭文怡1; 田 琼2; 王晓燕1
1.第四军医大学西京医院心内科，2.第四军医大学教学实验中心，陕西 西安 710032
Author(s):
HU Tao;  JIA Guo-liang;  LI huan;  WANG Hai-chang;  GUO Wen-yi;  TIAN Qiong;  WANG Xiao-yan
Department of Cardiology, Xijing Hospital, Fourth Military Medical University, Xi′an，Shaanxi 710032,China
关键词:
银杏叶提取物; 平滑肌细胞; 增殖
Keywords:
Gingko biloba extract;  smooth muscle cells;  lipid peroidation;  proliferation
分类号:
Q251;R541.4
DOI:
-
文献标识码:
A
摘要:
目的 观察银杏叶提取物(EGB) 对活性氧诱导的血管平滑肌细胞（VSMC）增殖的影响。方法 用含100 g/L小牛血清的DMEM体外培养大鼠主动脉平滑肌细胞，用不同浓度的过氧化氢（H2O2）作为外源性活性氧刺激VSMC增殖,应用3H胸腺嘧啶脱氧核苷（3H-TdR）掺入和细胞周期时相测定等方法观察给予不同浓度的EGB预处理后， EGB对H2O2作用后VSMC的增殖的影响。结果 H2O2对VSMC的增殖活性具有剂量依赖性促进作用，EGB可以抑制H2O2诱导的VSMC增殖,且具有剂量依赖性。结论 EGB对于动脉粥样硬化形成及经皮腔内冠状动脉介入治疗后再狭窄的防治可能具有应用前景。
Abstract:
AIM To investigate the effect of Gingko biloba extract (EGB) on vascular smooth muscle cell (VSMC) proliferation induced by reactive oxygen species. METHODS Rat aortic vascular smooth muscle cells were cultured in vitro with DMEM and 10 percent calf plasma. Different doses of H2O2 were added to the VSMC as extraneous reactive oxygen species. The effect of EGB on the VSMC proliferation was determined by3HTdR incorporation and cell cycle phase analysis after the cells were preincubated with different doses of EGB. RESULTS H2O2 exhibited dosedependent promotion of cell proliferative activity. EGB inhibited the effect of H2O2 on the proliferation of VSMC in a dosedependent manner. CONCLUSION EGB may have some potential clinical implication in the prevention and treatment of atherosclerosis and restenosis after percutaneous transluminal coronary angioplasty.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2005-08-01.作者简介：胡涛，主治医师，博士Tel: (029)84775183 Email: hutao74@Fmmu.edu.cn
更新日期/Last Update: