体外循环系统聚氯乙烯材料表面共价酯键结合肝素的方法(PDF)
《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]
- 期数:
- 2007年第1期
- 页码:
- 32-35
- 栏目:
- 基础研究
- 出版日期:
- 2007-01-01
文章信息/Info
- Title:
- Surface heparinization of polyvinyl chloride used for cardiopulmonary bypass system
- Author(s):
- DUAN Weixun1; YI Dinghua1; YANG Jian1; LIU Jincheng1; LIU Jian2; CAO Ruijun2
- 1.Department of Thoracic and Cardiovascular Surgery, Xijing Hospital, Fourth Military Medical University, Xi′an 710032, Shaanxi, China； 2.Department of Applied Chemistry, School of Science, Xi′an Jiaotong University, Xi′an 710049,Shaanxi,China
- Keywords:
- polyvinyl chloride(PVC); ester bonds; covalent bond; heparin
- 分类号:
- R318.08
- DOI:
- -
- 文献标识码:
- A
- 摘要:
- 目的 探讨体外循环系统聚氯乙烯材料表面共价结合肝素的方法，并对肝素化后聚氯乙烯材料的血液相容性及稳定性进行评估。方法 通过材料表面活化，引入活性位点放大剂及扩链剂，通过酯键将肝素分子共价结合到聚氯乙烯材料表面，并对肝素化后的聚氯乙烯材料进行肝素含量、肝素释放速率、体外抗凝血性能等检测。结果 肝素分子以酯键方式共价结合到聚氯乙烯材料表面，肝素结合量2～5 μg/cm2, 肝素释放速率测定显示该肝素化表面具有良好的稳定性，PT和APTT检测显示其具有良好的抗凝血性能。结论 通过酯键肝素共价键结合能提高聚氯乙烯材料表面肝素结合量，其稳定性和抗凝血性能良好。
- Abstract:
- AIM To explore a novel method to immobilize heparin onto the polyvinyl chloride (PVC) surface used for cardiopulmonary bypass system and to evaluate its bloodcompatibility and stability. METHODS Heparin was bonded to the PVC surface by urethane bonds after the use of amplification polymers. The surface heparin content, release velocity and ex vivo anticoagulation were then detected. RESULTS The surface heparin content was high about 2-5 μg/cm2. The release velocity result showed good stability. The product prolonged the PT and APTT significantly and had great anticoagulation activity. CONCLUSION This novel method can be used to combine the heparin onto PVC surface used for cardiopulmonary bypass system and to improve its biocompatibility.
参考文献/References
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备注/Memo
- 备注/Memo:
- 收稿日期: 2005-19-20.基金项目：西安市科学技术创新基金项目资助（No.ZS200215） 通讯作者：易定华，主任医师,主要从事人工心脏、冠心病研究Email:yidh@fmmu.edu.cn 作者简介：段维勋，博士生Email: duanweix@fmmu.edu.cn
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