自体心耳肌细胞移植对心功能的影响(PDF)
《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]
- 期数:
- 2007年第2期
- 页码:
- 170-172
- 栏目:
- 基础研究
- 出版日期:
- 2007-04-01
文章信息/Info
- Title:
- Effect of autologous auricle cardiomyocytes transplantation on cardiac function
- Author(s):
- ZHANG Hao1; CAI Zhenjie1; YU Shiqiang1; ZHAO Bijun1; ZHANG Jinbao1; Li li2; DUAN Yunyan2
- 1.Center of Cardiovascular Surgery, 2.Ultrasound Department,Xijing Hospital, Fourth Military Medical University, Xi′an 710032, Shaanxi , China
- 分类号:
- R541.4
- DOI:
- -
- 文献标识码:
- A
- 摘要:
- 目的 将急性分离的兔自体左心耳心肌细胞移植到自体梗死区心肌，以研究其对心功能的改善情况。方法 结扎成年兔的冠状动脉前降支，建立心肌梗死模型，4周后获取自体左心耳组织，急性消化分离为单细胞后分别将细胞悬液和培养液注射到移植组和未移植组梗死区内。4周后行超声心动图检查。 结果 4周后移植组与未移植组兔子全部存活。超声心动图检查，至移植后第4周，移植组左室功能各项指标好于未移植组。结论 急性分离的自体左心耳心肌细胞移植到梗死区心肌内能够改善左室功能。
- Abstract:
- AIM To investigate the effect of grafted autologous left auricle cardiomyocytes on cardiac function. METHODS We established MI models by ligating the left anterior descending artery. Four weeks later, the left auricle was harvested and the auricle heart cells were isolated and labeled with DAPI ex vivo. Either a cell suspension (transplant group) or culture medium (control group) was injected into the infarcted areas. All the rabbits were examined by 2D ultrasonic cardiogram (UCG) 4 weeks later. RESULTS The rabbits all survived four weeks after injection. UCG showed that the left ventricle function parameters in the transplant group were better than those in control group. CONCLUSION Isolated autologous left auricle cardiomyocytes improve left ventricular function after grafted into rabbit ventricular myocardium following myocardial infarction.
参考文献/References
［1］ Menasche P. Cell transplantation in myocardium［J］. Ann Thorac Surg,2003,75（6 suppl):S20-S28.
［2］ El Oakley RM,Ooi OC,Bongso A, et al. Myocyte transplantation for myocardial repair: a few good cells can mend a broken heart［J］. Ann Thorac Surg,2001,71(5):1724-1733.
［3］ Li RK,Mickle DA,Weisel RD,et al. Human pediatric and adult ventricular cardiomyocytes in culture: assessment of phenotypic changes with passaging ［J］. Cardiovasc Res,1996,32(2): 362-373.
［4］Dorfman J, Duong M, Zibaitis A ,et al. Myocardial tissue engineering with autologousmyoblast implantation［J］. J Thorac Cardiovasc Surg, 1998,116(5): 744 - 751.
［5］ Blatt A, Robinson D, Cotter G, et al. Improved regional left ventricular function after successful satellite cell grafting in rabbits with myocardial infarction ［J］. Eur J Heart Fail, 2003,5(6):751-757.
［6］ Zhang M, Methot D, Poppa V, et al. Cardiomyocyte grafting for cardiac repair: graft cell death and antideath strategies［J］. J Mol Cell Cardiol, 2001,33(5):907-921.
［7］ Kinoshita I , Vilquin JT , Tremblay JP. Pretreatment of myoblast cultures with basic fibroblast growth factor increases the efficacy of their transplantation in mdx mice［J］. Muscle Nerve, 1995,18(8):834-841.
［8］ Li RK, Mickle DA, Weisel RD,et al. In vivo survival and function of transplanted rat cardiomyocytes ［J］. Circ Res, 1996 ,78(2):283-288.
备注/Memo
- 备注/Memo:
- 收稿日期：2005-09-20.通讯作者：蔡振杰，教授， 主要从事大血管外科和心律失常的外科研究Email：Caizhenjie@hotmail.com作者简介：张浩，硕士生 Email：Zhanghaoo@hotmail.com
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