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|本期目录/Table of Contents|

NO及NOS在低氧大鼠肺组织和主动脉中的差异性及其意义(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2007年第3期
页码:
258-261
栏目:
基础研究
出版日期:
2007-06-01

文章信息/Info

Title:
Alteration of nitric oxide and nitric oxide synthase in lung and aorta in hypoxic pulmonary hypertensive rats
作者:
栾丽丽; 李志超; 黄玉芳; 董明清; 张博; 董海莹; 彭利静
第四军医大学病理生理学教研室，陕西 西安 710032
Author(s):
LUAN Lili;  LI Zhichao;  HUANG Yufang;  DONG Mingqing;  ZHANG Bo;  DONG Haiying;  PENG Lijing
Department of Pathophysiology, Fourth Military Medical University, Xi′an 710032, Shaanxi, China
关键词:
低氧性肺动脉高压; 肺; 主动脉; 一氧化氮; 一氧化氮合酶
Keywords:
hypoxic pulmonary hypertension;  lung ;  aorta ;  nitric oxide;  nitric oxide synthase
分类号:
R363.2
DOI:
-
文献标识码:
A
摘要:
目的 观察低氧性肺动脉高压大鼠肺组织匀浆、主动脉匀浆及出肺血和入肺血中一氧化氮（ＮＯ）的含量、一氧化氮合酶（ＮＯＳ）的活性。方法 将24只雄性SD大鼠随机分成4组,即常氧2周组、常氧3周组、低氧2周组、低氧3周组。采用间断负压低氧法制备大鼠低氧性肺动脉高压模型；右心室导管法测定最高肺动脉压（PAP）；左颈总动脉插管测量左颈总动脉压代表动脉血压（Psa）;计算右心室肥厚指数［RV/（LV+S）］；采用硝酸还原酶法测定各组大鼠出、入肺血及肺组织和主动脉匀浆中的NO含量；用化学比色法测定各组大鼠肺组织和主动脉匀浆中NOS的活性；应用免疫组化染色法观察各组大鼠肺组织及主动脉eNOS在蛋白质水平表达的变化。结果 低氧组大鼠的PAP［（43.4±4.4)mmHg，(51.8±4.2)mmHg,1 mmHg=0.133 kPa］, RV/(LV+S)（32.3±1.0，37.0±1.6）均高于其正常对照组［（20.8±2.4）mmHg，(21.8±3.9)mmHg;21.3±1.0，20.3±1.2,P<0.01）］,且随缺氧时间延长而增高（P<0.01），而Psa与常氧对照组比无差别。低氧组大鼠的出、入肺血及肺组织匀浆中的NO含量、NOS活性及肺组织eNOS的表达量均较其常氧对照组显著降低(P<0.01)，出肺血与入肺血的NO含量无差别；主动脉匀浆中NO含量和NOS的活性及大鼠主动脉的eNOS染色在各组间未见明显差异。结论 低氧时，大鼠肺组织中NO的含量及NOS的活性均较常氧时降低，而主动脉中二者的表达在低氧和常氧时却没有差异，这种差异性可能是低氧时引起肺动脉高压却很少导致高血压的机制之一。
Abstract:
AIM To observe the nitric oxide (NO) content and nitric oxide synthase (NOS) activity in lungs, aorta and blood from plasma of pulmonary artery (PA) and thoracic aorta (TA) in hypoxic pulmonary hypertension（HPH） rats. METHODS Twentyfour male SD rats were randomly divided into four groups with 6 rats in each group: normoxic 2 weeks group, normoxic 3 weeks group, hypoxic 2 weeks group and hypoxia 3 weeks group. The animal model of hypoxia pulmonary hypertension was established by intermittent hypoxia (imitation of highland 5000 m). The maximum pulmonary artery pressure (PAP max) and system artery pressure (Psa) were measured by right cardiac catheterization and by cannulating the left common carotid artery (LCCA), respectively. The right ventricle (RV) and left ventricle plus septum (LV+S) of rats were weighed. The method of nitrate reductase was used to determine the changes of NO and NOS activity in the plasma and tissue and immunohistochemistry was used to observe eNOS expression in pulmonary and aorta. RESULTS In the hypoxic groups, PAPmax［（43.4±4.4)mmHg, (51.8±4.2)mmHg］and the RV／(LV+S) （32.3±1.0, 37.0±1.6）increased significantly compared with those in the control groups［（20.8±2.4)mmHg, (21.8±3.9)mmHg, 21.3±1.0, 20.3±1.2, P<0.01］. The longer the time exposed to hypoxia, the higher these parameters (P<0.01), but there was no difference in Psa between hypoxic groups and normoxic groups. NO content and NOS activity in lung homogenate, plasma of PA and TA in HPH rats decreased significantly compared with those in the correspondingly control group (P<0.01) and no difference was found in the NO content of PA and TA. No difference of the NOS activity was found in aorta homogenate among the four groups. The positive expression of eNOS in pulmonary decreased in hypoxic groups compared with that in the normoxic groups but no difference was found in aorta. CONCLUSION NO content and NOS activity in lungs in HPH rats decrease significantly compared with those in control groups, and there is no difference in aorta. The alteration in lungs and aorta in HPH rats may offer some clues to why hypoxia often results in HPH and not hypertension.

参考文献/References

［1］ Perner A, Andresen L, Normark M, et al. Expression of nitric oxidesynthases and effects of Larginine and LNMMA on nitric oxide production and fluid transport in collagenous colitis［J］. Gut, 2001,49(3): 387-394.

［2］ Ozaki M, Kawashima S, Yamashita T, et al. Reduced hypoxic pulmonary vascular remodeling by nitric oxide from the endothelium［J］. Hypertension, 2001， 37(2):322 - 327.

［3］ Shirai M，Pearson JT，Shimouchi A ，et al．Changes in functional and histological distributions of nitric oxide synthase caused by chronic hypoxia in rat small pulmonary arteries［J］.Br J Pharmacol，2003，139(5):899-910．

［4］ 高景霞,叶红,李会革,等.组胺和低氧对猪肺动脉和主动脉内皮细胞eNOS基因表达的影响［J］. 中国病理生理杂志，2005，21（3）：459-464.

备注/Memo

备注/Memo:
收稿日期：2006-06-30. 通讯作者:李志超，教授，主要从事呼吸与循环病理生理研究Email:lizhic@fmmu.edu.cn 作者简介:栾丽丽，助教，硕士生Email:luanlili620@yahoo.com.cn
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