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雄激素对离体大鼠心脏缺血/再灌注心功能及心肌凋亡的影响(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2007年第3期
页码:
272-276
栏目:
基础研究
出版日期:
2007-06-01

文章信息/Info

Title:
Effects of testosterone on postischemic cardiac functional recovery and myocardial apoptosis in rats
作者:
张中1; 郑强荪1; 马恒2; 陈迈3; 高渊1; 雷靖袆1; 张超1; 刘文秀1;  张宪伟1
第四军医大学:1.唐都医院心血管内科，陕西 西安 710038，2.生理学教研室， 陕西 西安 710032，3.西京医院心脏内科
Author(s):
ZHANG Zhong1;  ZHENG Qiangsun1;  MA Heng2;  CHEN Mai3;  GAO Yuan1;  LEI Jingyi1;  ZHANG Chao1;  LIU Wenxiu1;  ZHANG Xianwei1
1.Department of Cardiology, Tangdu Hospital, Fourth Military Medical University, Xi’an 710038, Shaanxi, China,2.Department of Physiology, Fourth Military Medical University, Xi’an 710032, Shaanxi, China,3.Department of Cardiology, Xijing Hospital
关键词:
雄激素;  缺血/再灌注;  心肌梗死;  凋亡
Keywords:
testosterone;  ischemia / reperfusion;  myocardial infarction;  apoptosis
分类号:
R542.2
DOI:
-
文献标识码:
A
摘要:
目的 观察雄激素对离体大鼠缺血/再灌注心脏功能和心肌细胞凋亡的影响。方法 SD大鼠42只，随机分为雄激素组（TUD 500 mg/kg）、对照组、去势组，每组14只。制备大鼠离体缺血/再灌注模型（缺血30 min，再灌注2 h），观察左室压力，再灌注结束后检测心肌梗死率和细胞凋亡指数，免疫组化技术检测心肌组织的Bcl2、Bax和Caspase3水平。结果 ① 与对照组、去势组相比，雄激素组可以明显改善再灌注后的心脏左室收缩及舒张功能（P<0.05）； ② 雄激素组心肌梗死范围(31±11）%比对照组(39±8）%和去势组(44±7）%显著减少（均P<0.05）； ③ 雄激素抑制心肌细胞凋亡的发生［(3.7±1.1)% vs (5.8±0.9)%、(6.5±2.3)%,均 P<0.05］，增加心肌组织中Bcl2含量，Bax含量未见显著变化，Caspase3激活减少，有统计学意义（P<0.05）。结论 雄激素对离体心脏缺血/再灌注有保护作用，心肌细胞凋亡为其发挥保护作用的机制之一。
Abstract:
AIM To study the effects of testosterone on the postischemic cardiac recovery and myocardial apoptosis in rats. METHODS Nonorchiectomized mature male SD rats were randomly assigned to control, a single dose of testosterone undecanoate (TUD), or orchiectomized group. After 2 weeks of treatment, the hearts were removed and placed in a Langendorff setup. The left anterior descending coronary arteries of the isolated, bufferperfused hearts were occluded for 30 min, followed by 120 min reperfusion. The recovery of myocardial function was measured by analyzing preand postischemic left ventricular (LV) systolic/diastolic pressure simultaneously, and myocardial infarction and cardiac myocyte apoptosis were determined at the end of reperfusion. Bcl2, Bax and Caspase3 protein were analyzed by immunohistochemical method. RESULTS The recovery of contractile function was improved in testosteronetreated rats: at the end of the reperfusion, LV systolic pressure was higher and enddiastolic pressure was lower in testosteronetreated rats. Compared with control group and orchiectomized group, testosteronetreated rats significantly reduced myocardial infarction (31%±11% vs 39%±8%, 44%±7%) and decreased apoptosis index（3.7%±1.1% vs 5.8%±0.9%, 6.5%±2.3%）at the end of reperfusion. The Bcl2 expression increased and the activated Caspase3 deceased after TUD administration, but no significant difference in Bax protein expression was found between the three groups. CONCLUSION Administration of testosterone significantly improves the recovery from ischemia. These beneficial effects are associated with an attenuation of ischemia/reperfusion induced myocardial infarction and apoptosis.

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备注/Memo

备注/Memo:
收稿日期：2006-09-01.通讯作者：郑强荪，教授， 主要从事心血管电生理研究Email：qingshu@fmmu.edu.cn作者简介：张中，主治医师 硕士生Email:zhzhong@fmmu.edu.cn
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