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Title:: 9cisRA inhibits expression of MMP9 in THP1 human monocytes induced by PMA

作者:: 周磊¹ ; 曾锦章²; 沈玲红¹; 王力²; 胡刘华¹; 何奔¹; 1.上海交通大学医学院附属仁济医院心内科,上海 200001; 2.上海生命科学研究院,上海 200233

Author(s):: ZHOU Lei¹; ZENG Jinzhang²; SHEN Linghong¹; WANG Li²; HU Liuhua¹; HE Ben¹; 1.Department of Cardiology, Renji Hospital, Shanghai Jiaotong University, Shanghai 200001, China; 2.Shanghai Institutes for Biological Sciences, Shanghai 200233, China

关键词:: 9顺式维甲酸; 视黄醛X受体; 基质金属蛋白酶9; 动脉粥样硬化

Keywords:: 9cisRetinoic Acid; retinoid X receptors; matrix metalloproteinase9; atherosclerosis

分类号:: R329.26

DOI:: -

文献标识码:: A

摘要:: 目的探讨视黄醛X受体（retinoid X receptors ，RXRs）特异性激动剂9顺式维甲酸（9cisRA）对佛波酯（PMA）诱导人单核细胞系THP1基质金属蛋白酶9（MMP9）表达及活性的影响。方法体外培养THP1细胞，PMA诱导分化为巨噬细胞，采用9cisRA对不同浓度PMA组进行干预，应用RealtimePCR、Westerblotting测定THP1细胞MMP9的基因和蛋白水平表达水平，通过Gelatin Zymography法检测MMP9的酶活性。结果 9cisRA（100 nmol/L）对不同浓度PMA组（10、20和40 nmol/L）干预24 h，9cisRA 可明显抑制THP1细胞MMP9转录水平, MMP9 的mRNA抑制率分别28%、60%、88%（P<0.01）。MMP9蛋白水平及酶活性也呈显著下降。结论 RXRs 特异性激动剂9cisRA可显著抑制PMA诱导THP1的MMP9转录和蛋白水平表达及其酶活性。

Abstract:: AIM To investigate the effect of 9cisRA (9cisRetinoic Acid), a specific agonist of retinoid X receptors (RXRs), on the expression and activities of matrix metalloproteinase9 (MMP9) in THP1 human monocytes induced by PMA. METHODS Human monocytic cell lines THP1 were cultured and induced to macrophages by PMA treatment. The differentiated monocytes were then cultured in the medium of PMA at different concentrations treated with 9cisRA (100 nmol/L) and the gene expression, protein expression and activity of MMP9 were detected by RealtimePCR, Western blotting and Gelatin Zymography respectively. RESULTS After 24 h treatment of 100 nmol/L 9cisRA, the mRNA level of MMP9 in THP1 cells induced by PMA at different concentrations (10, 20 and 40 nmol/L) decreased by 28%, 60%, 88% (P<0.01) respectively, while the protein level and activity of MMP9 also dropped significantly. CONCLUSION 9cisRA, a specific agonist of RXRs, effectively inhibits the mRNA, protein level and activity of MMP9 in THP1 cells induced by PMA.

参考文献/References

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9顺式维甲酸抑制PMA诱导人单核细胞系THP1表达MMP9(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

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