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Title:: Bone marrow mononuclear cells transplantation inhibits rat cardiac myocyte apoptosis after acute myocardial infarction

作者:: 张瑞成¹; 2; 董念国¹ ; 侯剑峰²; 法宪恩²; 1.华中科技大学附属协和医院心血管外科，湖北武汉 430022；2.郑州大学第二附属医院心血管外科，河南郑州 450014

Author(s):: ZHANG Ruicheng¹; 2; DONG Nianguo¹; HOU Jianfeng²; FA Xianen²; 1.Department of Cardiovascular Surgery, Union Hospital Affiliated to Huazhong University of Science and Technique, Wuhan 430022, Hubei, China; 2.Department of Cardiovascular Surgery, Second Affiliated Hospital of Zhengzhou University, Zhengzhou 450014, Henan, China

关键词:: 骨髓干细胞; 细胞移植; 心肌梗死; 细胞凋亡

Keywords:: bone marrow mononuclear cells; cell transplantation; myocardial infarction; apoptosis

分类号:: R813；R542.22

DOI:: -

文献标识码:: A

摘要:: 目的观察骨髓单个核细胞(bone marrow mononuclear cells, BMMNCs)移植对大鼠急性心肌梗死（AMI）后心肌细胞凋亡的影响。方法健康雄性Wistar大鼠30只，随机均分为实验组和对照组。用结扎冠状动脉左前降支的方法建立大鼠AMI模型，将制备的BMMNCs悬液和培养液分别经心外膜下植入实验组和对照组梗死心肌周围。移植术后4周末，检测心肌细胞凋亡情况及Bcl2、Fas、FasL蛋白在心肌细胞中表达水平,并观察梗死区心肌内移植BMMNCs及其周边区组织形态学特点。结果移植后4周末，实验组心肌细胞凋亡指数显著低于对照组(P<0.05)；与对照组相比，实验组Bcl2蛋白吸光值显著升高（P<0.05），Fas、FasL蛋白吸光值显著降低（P<0.05）；实验组心肌梗死区内有BrdU标记阳性的BMMNCs存活。结论同种异体大鼠BMMNCs移植可调节Bcl2、Fas、FasL蛋白表达，抑制AMI后心肌细胞凋亡发生。

Abstract:: AIM To observe the effects of allogeneic bone marrow mononuclear cells （BMMNCs）on rat cardiac myocyte apoptosis after acute myocardial infaretion(AMI). METHODS Thirty male healthy Wistar rats were randomly divided into two groups: control group（n=15）and transplantation group（n=15）. As soon as the descending anterior branch of left coronary artery was ligated, DMEMLG medium was injected into the periinfarct area of control group and BMMNCs labeled with BrdU were injected into the periinfarct area of transplantation group. Apoptotic cells and the expression of Bcl2, Fas and FasL were detected 4 weeks later. RESULTS Four weeks after the transplantation, cardiomyocyte apoptotic index and the expression of Fas and FasL decreased significantly（P<0.05） while the expression of Bcl2 increased significantly（P<0.05）in the transplantation group, compared with those in the control group. BMMNCs labeled with BrdU were observed around the injected sites by immunohistochemical examination. CONCLUTION Allogeneic BMMNCs transplantation regulate the expression of Bcl2, Fas and FasL and inhibits cardiomyocyte apoptosis after AMI.

参考文献/References

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骨髓干细胞移植可影响心肌梗死后心肌细胞的凋亡(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

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