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|本期目录/Table of Contents|

对氧磷酶1基因192位Gln/Arg多态性对冠心病患者内皮功能的影响(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2007年第5期
页码:
550-552
栏目:
临床研究
出版日期:
2007-10-01

文章信息/Info

Title:
Effect of paraoxonase gene 192 Gln/Arg polymorphismin on vascular endothelial function in patients with coronary heart disease
作者:
魏立业; 夏岳; 戚国庆; 张庆文
河北医科大学第一医院心内三科，河北 石家庄 050031
Author(s):
WEI Liye;  XIA Yue;  QI Guoqing;  ZHANG Qingwen
Department of Cardiology, First Affiliated Hospital, Hebei Medical University, Shijiazhuang 050031, Hebei, China
关键词:
冠状动脉疾病; 对氧磷酶; 基因; 多态性; 血管内皮功能
Keywords:
coronary disease;  paraoxonase;  gene;  polymorphism;  vascular endothelial function
分类号:
R541.4
DOI:
-
文献标识码:
A
摘要:
目的 研究国人血清对氧磷酶1（PON1）基因192位Gln/Arg多态性对冠心病患者血管内皮功能的影响。方法 应用聚合酶链式反应限制性片断长度多态性（PCRRFLP）方法，对石家庄地区汉族151例冠心病患者及91例正常对照者PON1基因192位Gln/Arg多态性进行分析，同时应用超声检测肱动脉内皮功能。结果 冠心病组及正常对照组PON1基因均以QR基因型为主，其频率分别为48%和54%。冠心病组RR型基因频率高于对照组（P<0.05）。平衡法计算等位基因的频率，R等位基因在冠心病组明显增高（65% vs 39%，P<0.05）。冠心病组PON1各基因型内皮依赖性舒张功能均低于对照组（P<0.05），以RR基因型最为明显，其次为QR基因性；而两组非内皮依赖性舒张功能差异无显著性。结论 PON1基因192位Gln/Arg多态性可能与冠心病患者血管内皮功能异常有关。R等位基因可能为血管内皮功能受损的相对危险因素，且参与冠心病的发病。
Abstract:
AIM To observe the effect of Paraoxonase (PON1) gene 192 Gln/Arg polymorphism on vascular endothelial function in patients with coronary heart disease in Chinese population. METHODS The genotype and allele frequency of PON1 gene 192 Gln/Arg polymorphism were assayed bypolymerase chain reaction (PCR)restriction fragment length polymorphism (RFLP) and the vascular endothelial function was examined with ultrasound technique in 151 CHD patients and 91 healthy subjects from a population of Chinese Han nationality in Shijiazhuang area. RESULTS In both CHD group and control group, PON1 gene was mostly of QR genotype and its frequencies were 48% and 54%. The frequency of R allele of PON1 gene in CHD group was significantly higher than that in control group (65% vs 39%, P<0.05). The CHD patients with individual PON1 genotype had a depressed endotheliumdependent vasodilation function and this was more significant in the RR genotype group. No difference endotheliumindependent vasodilation function was found between the two groups. CONCLUSION PON1 gene 192 Gln/Arg polymorphism may be associated with abnormal vascular endothelial function. R allele of PON1 gene may be a relative risk factor and be associated with the episode of CHD.

参考文献/References

［1］ Mackness MI, Mackness B, Durington PN, et al. Paraoxonase and coronary heart disease［J］. Curr Opin Lipidol, 1998, 9(4): 319-324.

［2］ Pfohl M, Koch M, Enderle MD, et al. Paraoxonase 192Gln/Arg gene polymorphism, coronary artery disease, and myocardial infarction in type2 diabetes［J］. Diabetes, 1999, 48(3): 623-627.

［3］ Parzer S, Mannhalter C. A rapid method for the isolation of genome DNA from citrate whole blood［J］. Biochem J, 1991, 273(pt 1): 229-231.

［4］ Celermajer DS, Sorensen KE, Gooch VM, et al. Non-invasive detection of endothelial dysfunction in children and adults at risk of atherosclerosis［J］. Lancet,1992, 340(8828) : 1111-1115.

［5］ Ross R. Atherosclerosisan inflammatory disease［J］. N Engl J Med, 1999, 340(2): 115-126.

［6］ Anderson YJ, Uehata A, Gerhard MD, et al. Close relation of endothelial function in the human coronary and peripheral circulation ［J］. J Am Coll Cardiol, 1995, 26(5):1235-1241.

［7］ Sanghera D K, Aston C E, Saha N, et al . DNA polymorphism in two paraoxonase genes (PON1 and PON2) are associated with the risk of coronary heart disease［J］. Am J Hum Genet, 1998,62 (1) : 36-44.

［8］ 尤蓓，于金德，陆林，等.中国人群2型糖尿病患者对氧酶基因多态性与冠心病关系的研究［J］. 中华内分泌代谢杂志，2000,16(1):3-5.

［9］ 刘康桐，曾高峰，刘革修,等. 国人血清对氧磷酶192Gln/Arg多态性及其活性与冠心病发病的关系［J］. 临床心血管病杂志，2000,16(4):148-150.

［10］钱庆文，钱书虹，王诗瑾. 郑州地区汉族人群2型糖尿病并发冠心病患者与对氧磷脂酶/芳香酯酶192位基因多态性的关系［J］. 临床心血管病杂志，2003,19(10):606-609.

备注/Memo

备注/Memo:
收稿日期：2006-09-11.基金项目：河北省卫生厅基金课题资助（No.03183）通讯作者：夏岳，主任医师，教授，主要从事冠心病及其介入治疗Email:ydyy@yahoo.com.cn作者简介：魏立业，医师，硕士Email:weiliye2000@yahoo.com.cn
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