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心肌营养素-1对三个主要心肌转录因子转录表达的选择性的研究(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2007年第6期
页码:
621-625
栏目:
基础研究
出版日期:
2007-12-20

文章信息/Info

Title:
Effect of cardiotrophin-1 on transcriptional expression of three main transcriptional factors
作者:
赵赫男1; 姜妙娜1; 张彩华 1; 李深 1; 冯晓莹2; 贾玉杰1
大连医科大学：1病理生理学教研室，辽宁 大连116027; 2 附属第二医院
Author(s):
ZHAO He-nan;  JIANG Miao-na;  ZHANG Cai-hua;  LI Shen;  FENG Xiao-ying;  JIA Yu-jie
Department of Pathophysiology, Dalian Medical University, Dalian 116027, Liaoning, China
关键词:
心肌营养素-1; 心肌转录调节因子GATA4; 心肌肥厚; 信号转导
Keywords:
cardiotrophin-1; GATA4; NKX2-5; MEF2; cardiac hypertrophy; signal transduction
分类号:
R318.11
DOI:
-
文献标识码:
A
摘要:
目的 研究心肌营养素-1（cardiotrophin-1，CT-1）对三大主要心肌转录因子的选择性作用，在转录因子水平探讨CT-1肥大刺激作用的特殊机制。 方法 应用半定量RT-PCR及免疫组化技术，鉴定CT-1对心肌细胞形态学影响。测定CT-1对大鼠心肌细胞GATA4、MEF2以及NKX2-5基因转录的时间、剂量依赖影响。结果GATA4与NKX2-5的表达均与CT-1具有时间、剂量依赖关系，而CT-1则未对MEF2的表达产生影响。从作用时间上看， NKX2-5的表达在1×10-10 mol/L的CT-1刺激1 h时即开始明显增加，而GATA4的表达则在3 h时显著性增加，NKX2-5的表达增强的时间较GATA4略早。结论CT-1以时间和剂量依赖性的方式上调大鼠心肌细胞GATA4及NKX2-5 mRNA表达，对大鼠心肌细胞MEF2 mRNA表达没有明显影响。
Abstract:
AIM To study the selective effects and mechanism of cardiotrophin-1 (CT-1) on the three main cardiac transcriptional factors. METHODS Using semiquantity RT-PCR and immunohistochemistry method, we identified the morphologic effects of CT-1 on cardicmyocytes. RESULTS Time and dose dependent relationship was found between CT-1 and GATA4 or NKX2-5. CT-1 did not affect the expression of MEF2 mRNA. The expression of NKX2-5 mRNA significantly increased 1h after treatment with 1×10-10 mol/L CT-1, while significant increase were found in GATA4 mRNA only 3h after the treatment with the same concentration of CT-1. The increasing of NKX2-5 was earlier than that of GATA4. CONCLUSIONCT1 promotes the expression of GATA4 or NKX2-5 mRNA in a time and dosedependent manner but has no obvious effect on mRNA expression of MEF2.

参考文献/References

［1］ Akazawa H, Komuro I. Roles of cardiac transcription factors in cardiac hypertrophy［J］. Circ Res, 2003, 92(10): 1079-1088.

［2］ Molkentin JD. The zinc finger-containing transcription factors GATA4, 5, and 6: ubiquitously expressed regulators of tissuespecific gene expression［J］. J Biol Chem, 2000, 275(50): 38949-38952.

［3］ Bruneau BG. Transcriptional regulation of vertebrate cardiac morphogenesis［J］. Circ Res, 2002, 90(5): 509-519.

［4］ Zhu Z, Zhu S, Liu D, et al. GATA4mediated cardiac hypertrophy induced by dmyoinositol 1,4,5trisphosphate［J］. Biochem Biophys Res Commun, 2005, 338(2):1236-1240.

［5］ Akazawa H, Komuro I. Cardiac transcription factor Csx/Nkx25: Its role in cardiac development and diseases［J］. Pharmacol Ther, 2005, 107(2):252-268.

［6］ Czubryt MP, Olson EN. Balancing contractility and energy production: the role of myocyte enhancer factor 2 (MEF2) in cardiac hypertrophy［J］. Recent Prog Horm Res, 2004, 59(1):105-124.

[7］ Pu WT, Ma Q, Izumo S. NFAT transcription factors are critical survival factors that inhibit cardiomyocyte apoptosis during phenylephrine stimulation in vitro［J］. Circ Res, 2003,92(7):725-731.

［8］ Kuwahara K, Saito Y, Harada M, et al. Involvement of cardiotrophin1 in cardiac myocytenonmyocyte interactions during hypertrophy of rat cardiac myocytes in vitro［J］. Circulation, 1999, 100(10): 1116-1124.

［9］ Slomiany BL, Slomiany A. Srckinasedependent epidermal growth factor receptor transactivation in salivary mucin secretion in response to betaadrenergic Gproteincoupled receptor activation［J］. Inflammopharmacology, 2004, 12(3):233-245.

［10］ Ghanouni P, Steenhuis JJ, Farrens DL, et al. Agonistinduced conformational changes in the Gproteincoupling domain of the beta 2 adrenergic receptor［J］. Proc Natl Acad Sci USA, 2001,98(11):5997-6002.

［11］You HW, Chen X, You HJ, et al. Role of endothelin1 and its receptors on hypertrophy or proliferation of cultured cardial cells［J］. Zhongguo Yi Xue Ke Xue Yuan Xue Bao, 2006, 28(4):520-523.

［12］Zhai P, Galeotti J, Liu J, et al. An angiotensin II type 1 receptor mutant lacking epidermal growth factor receptor transactivation does not induce angiotensin IImediated cardiac hypertrophy［J］. Circ Res, 2006, 99(5): 528-536.

［13］Lien CL, Wu C, Mercer B, et al. Control of early cardiacspecific transcription of Nkx25 by a GATAdependent enhancer［J］. Development, 1999, 126(1): 75-84.

［14］Shiojima I, Komuro I, Oka T, et al. Contextdependent transcriptional cooperation mediated by cardiac transcription factors Csx/Nkx25 and GATA4［J］. J Biol Chem, 1999, 274(12): 8231-8239.

［15］Zhu H, Garcia AV, Ross RS, et al. A conserved 28basepair element (HF1) in the rat cardiac myosin lightchain2 gene confers cardiacspecific and αadrenergicinducible expression in cultured neonatal rat myocardial cells［J］. Mol Cell Biol, 1991, 11(4): 2273-2281.

备注/Memo

备注/Memo:
收稿日期：2006-11-03.通讯作者：贾玉杰，教授，主要从事肝纤维化机制的研究 Email：pathophy@163.com 作者简介：赵赫男，讲师，博士 Email：johnala_cn@sina.com.
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