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|本期目录/Table of Contents|

粒细胞集落刺激因子对大鼠颈总动脉损伤后新生内膜增生的影响(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2007年第6期
页码:
635-637/641
栏目:
基础研究
出版日期:
2007-12-20

文章信息/Info

Title:
Effects of granulocyte colonystimulating factor on neointimal formation in catheter injured common carotid artery of atherosclerotic rats
作者:
尹小燕; 胡萍; 蔡文玮; 盛净
上海交通大学医学院附属第九人民医院老年病科，上海 200011
Author(s):
YIN Xiao-yan;  HU Ping;  CAI Wen-wei;  SHENG Jing
Department of Geriatrics, Shanghai No.9 People′s Hospital, Shanghai Jiaotong University Medical School, Shanghai 200011, China
关键词:
粒细胞集落刺激因子; 再狭窄; 动脉粥样硬化
Keywords:
granulocyte colonystimulating factor;  restenosis;  atherosclerosis
分类号:
R543.5
DOI:
-
文献标识码:
A
摘要:
目的 探讨粒细胞集落刺激因子(G-CSF)对动脉粥样硬化(AS)大鼠颈总动脉球囊损伤后再狭窄的影响。方法 Wistar大鼠48只，随机分为3组：普通饮食组（普食组，n=6），高脂饮食组（高脂组，n=42）。高脂组又分为：G-CSF组(n=21)和生理盐水组（NS组，n=21）。高脂饮食后1个月用HE染色及透射电镜证明AS形成情况。G-CSF组和NS组分别于高脂饮食后1个月给予G-CSF或生理盐水干预6 d，然后行左颈总动脉球囊损伤术，术后14、21、90 d分别用扫描电镜、HE染色及图像分析观察血管内皮形成及内膜的增生情况。结果 高脂组大鼠高脂饮食后1个月可见AS的早期改变，行球囊损伤后可见大鼠颈总动脉内膜逐渐增厚，但是G-CSF组明显小于NS组，G-CSF组损伤血管内皮较快恢复连续性，G-CSF组内膜与中膜面积比值（Ai/Am）远小于NS组（P<0.01）。结论 G-CSF可以加速AS大鼠损伤血管的再内皮化、抑制血管损伤后再狭窄的发生。
Abstract:
AIM To explore the influence of granulocyte colonystimulating factor (GCSF) on neointimal formation in catheter injured common carotid artery of atherosclerotic rats. METHODS Forty eight Wistar rats were randomly divided into: control group (n=6) on normal diet and high cholesterol group (n=42) on high cholesterol diet. The high cholesterol group was subdivided into G-CSF group (n=21) and NS group (n=21) I month later. Rats in G-CSF group were injected subcutaneously with recombinant human G-CSF for 6 days, while those in NS group injected subcutaneously with saline the same volume as G-CSF for 6 days. The vascular injury models were created by balloon catheter in the common carotid arteries of rats. The samples were studies with scanning electron microscopy, transmission electron microscopy and stained with hematoxylin and eosin. RESULTS The atherosclerotic rat model was successfully made. In the vascular injury model, GCSF significantly reduced the restenosis score of common carotid arteries and prevented an increase in neointima/media ratio at 14, 21 and 90 days after the treatment in Wistar rats. CONCLUSION G-CSF has some therapeutic potentials for the progression of restenosis in atherosclerotic rats.

参考文献/References

［1］ Strehlow K, Werner N, Berweiler J, et al. Estrogen increases bone marrow derived endothelial progenitor cell production and diminishes neointima formation［J］. Circulation,2003,107(24):3059-3065.

［2］ Hur J, Yoon CH, Kim HS, et al. Characterization of two types of endothelial progenitor cells and their different contributions to neovasculogenesis［J］. Arterioscler Thromb Vasc Biol,2004,24(2):288-293.

［3］ Harada M, Qin Y, Takano H, et al. GCSF prevents cardiac remodeling after myocardial infarction by activating the JakStat pathway in cardiomyocytes［J］. Nat Med,2005，11(3):305–311.

［4］ Kong D, Melo LG, Gnecchi M, et al. Cytokineinduced mobilization of circulating endothelial progenitor cells enhances repair of injured arteries［J］. Circulation，2004，110(14):2039-2046.

［5］ Yoshioka T, Takahashi M, Shiba Y, et al. Granulocyte colonystimulating factor (GCSF) accelerates reendothelialization and reduce neointimal formation after vascular injury in mice［J］. Cardiovascular Res，2006，70(1):61-69.

［6］ Kang,HJ, Kim HS, Zhang SY, et al, Effects of intracoronary infusion of peripheral blood stemcells mobilized with granulocytecolony stimulating factor on left ventricular systolic function and restenosis after coronary stenting in myocardial infarction: the MAGIC cell randomized clinical trial［J］. Lancet，2004，363(9411)：751-756.

［7］ Simper D, Stalboerger PG, Panetta CJ, et al. Smooth muscle progenitor cells in human blood［J］. Circulation，2002，106（10）:1199-1204.

备注/Memo

备注/Memo:
收稿日期：2006-09-08.作者简介：尹小燕，硕士生 Email：yinxiaoyan80@126.com
更新日期/Last Update: