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血管紧张素Ⅱ对心肌缝隙连接蛋白43重构的影响及(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2008年第1期
页码:
21-23
栏目:
基础研究
出版日期:
2008-01-20

文章信息/Info

Title:
Effect of angiotensinⅡand benazepril on remodeling of cardiac gap junction connexin43
作者:
张卫泽1邵鹏2陈永清1马凌1李富军1尹强1
1.兰州军区总医院心内科,甘肃 兰州 730050 ;2. 甘肃省第二人民医院内四科,甘肃 兰州 730000
Author(s):
ZHANG Weize1 SHAO Peng2 CHEN Yongqing1 MA Ling1 LI Fujun1 YIN Qiang1
1.Department of Cardiology, General Hospital, Lanzhou Military Area Command, Lanzhou 730050,Gansu, China;2.Department of Cardiology, Second People’s Hospital of Gansu Province, Lanzhou 730000,Gansu, China
关键词:
血管紧张素Ⅱ缝隙连接蛋白43贝那普利重构
Keywords:
angiotensin Ⅱgap junction connexin43benazeprilremodeling
分类号:
R541.7;R331.38
DOI:
-
文献标识码:
A
摘要:
目的 采用大鼠肾上腹主动脉部分结扎的模型,观察心肌缝隙连接蛋白(Connexin,Cx)43含量和分布的变化以及贝那普利干预的防治效果。方法 将30只雄性Wistar大鼠随机等分为对照组(A组)、腹主动脉结扎组(B组)、结扎+贝那普利组(C组)。喂养8周后处死,用放射免疫法测定血浆血管紧张素Ⅱ(AngⅡ)浓度和心肌组织AngⅡ浓度,用免疫组化方法显示大鼠心肌Cx43的分布特征,半定量统计分析。结果 与A组相比,B组大鼠血浆、心肌AngⅡ浓度显著升高(均P<0.01),但B组大鼠心房肌及心室肌Cx43含量均较A组显著减少(分别为P<0.05和P<0.01),且分布紊乱;而C组Cx43含量较B组显著增多(P<0.01),且分布不规律程度减轻。结论 AngⅡ升高可能导致心肌Cx43重新分布及表达减少;贝那普利能有效减轻Cx43的重构。
Abstract:
AIM To investigate the effect of angiotensinⅡ(AngⅡ)and benazepril on the level and distribution changes of connexin43(Cx43) in rats with suprarenal abdominal aorta banded. METHODS Thirty male Wistar rats were randomly divided into control group(A), abdominal aorta banded group(B) and abdominal aorta banded plus benazepril group(C). The plasma and myocardium concentration of AngⅡ was determined by radioimmunoassay after eight weeks. Cx43 was detected with immunohistochemical method and was semiquantitatively analyzed in different groups. RESULTS The plasma and myocardium concentration of Ang Ⅱ increased markedly(P<0.01)but the Cx43 protein level of atrial and ventricular tissue in group B decreased significantly(P<0.05 and P<0.01 , respectively) with misdistribution compared with those in group A . Cx43 level increased significantly(P<0.01)in group C compared with that in group B and the inhomogeneity in Cx43 distribution decreased. CONCLUSION The results show that Cx43 remodeling with redistribution and reduction of protein expression may be induced by the increase of Ang Ⅱlevel and benazepril attenuates Cx43 remodeling effectively.

参考文献/References

[1] 刘英明,卢才义,杜新平,等. 腹主动脉结扎大鼠房性快速心律失常诱发率升高[J]. 中国心脏起搏与心电生理杂志, 2004, 18(3): 195-197.

[2] 张家明,李大强,于世龙,等.坎地沙坦对家兔快速心房刺激所致缝隙连接蛋白40重构的影响[J]. 中国心脏起搏与心电生理杂志, 2005, 19(5): 385-387.

[3] Li WE, Nagy JI. Connexin43 phosphorylation state and intercellular communication in cultured astrocytes following hypoxia and protein phosphatase inhibition [J]. Eur J Neurosci, 2000, 12 (7): 2644-2650.

[4] Ruch RJ, Trosko JE, Madhukar BV. Inhibition of connexin43 gap junctional intercellular communication by TPA requires ERK activation [J]. J Cell Biochem, 2001, 83(1): 163-169.

[5] 张韬,杨庆,邓珏琳,等. 心室肥厚时的缝隙连接蛋白Cx43及快速刺激对其的影响[J]. 中国心脏起搏与心电生理杂志, 2002, 16(2): 136-138.

[6] 邵素霞,刘岩,赵春芳,等.高血压大鼠心脏中缝管连接蛋白的表达及连接结构观察[J].中国老年学杂志, 2003, 23(9): 598-600.

[7] Saffitz JE, Green KG, Kraft WJ , et al. Effects of diminished expression of connexin 43 on gap junction number and size in ventricular myocardium [J] . Am J Physiol Heart Circ Physiol, 2000,278(5):H1662-H1670.

备注/Memo

备注/Memo:
收稿日期:2006-12-19.基金项目:甘肃省自然科学基金项目资助(3ZS041A25057)通讯作者:张卫泽,主任医师,主要从事心脏电生理及介入心脏病研究Email: zhangzwz@medmail.com.cn
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