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氧自由基对脂肪细胞中脂联素表达的下调作用 (PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2008年第2期
页码:
142-146
栏目:
基础研究
出版日期:
2008-03-20

文章信息/Info

Title:
Expression of adiponeckine is downregulated by reactive oxygen species in adipocytes
作者:
陈宝莹1魏经国1王玮1Xu Amin2于军3
第四军医大学:1. 唐都医院放射科,陕西 西安 710038,3.教学实验中心,陕西 西安 710032;2.香港大学内科系,香港
Author(s):
CHEN Baoying1 WEI Jingguo1 WANG Wei1 XU Amin2 YU Jun3
1.Department of Radiology, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, Shaanxi, China; 2.Department of Medicine, University of Hong Kong, Hong Kong; 3.Experiment Teaching Center, Fourth Military Medical University, Xi'an 710032, Shaanxi, China
关键词:
低氧氧自由基脂联素脂肪细胞
Keywords:
hypoxia reactive oxygen species adiponectin adipocyte
分类号:
R329.25
DOI:
-
文献标识码:
A
摘要:
目的 研究脂肪细胞中氧自由基(reactive oxygen species,ROS)对脂联素(adiponectin,AD)表达的调节,并探讨其机制。方法 培养3T3L1细胞,并诱导其分化为脂肪细胞,以MTT比色法检测细胞的活性。采用HydroethidineTM(HE)及H2DCFDA测定细胞内ROS的产生。分别以定量PCR、多重免疫分析及夹心ELISA法,检测AD mRNA和其蛋白的表达水平。结果 低氧条件诱导脂肪细胞中ROS产生。作为一种重要的ROS,H2O2可剂量依赖性地抑制3T3L1脂肪细胞产生AD,并降低AD基因的转录激活因子增强子结合蛋白α(CCAAT/enhancer binding protein alpha,C/EBPα)及过氧化物酶体增殖激活受体γ(peroxisome proliferatoractivated receptor gamma,PPARγ)的表达。结论 低氧产生ROS,而ROS导致脂肪细胞中AD的表达抑制,其重要机制之一是ROS可下调C/EBPα和PPARγ,从而减弱AD基因的转录激活。
Abstract:
AIM To investigate the regulatory effects of ROS (reactive oxygen species) on the production of AD (adiponectin) in adipocytes and try to determine the underlying mechanisms. METHODS 3T3L1 cells were cultured and differentiated into mature adipocytes, and cell viability was measured by MTT. Intracellular ROS production was detected by HydroethidineTM(HE)and H2DCFDA. AD gene expression level was evaluated by quantitative realtime PCR. Quantification of the AD protein level secreted into conditioned medium was performed by multiplex immunoassay and sandwich ELISA. RESULTS In 3T3L1 adipocytes,the production of intracellular ROS was enhanecd by hyposia. As an important ROS, H2O2 dosedependently decreased AD production in 3T3L1 adipocytes. H2O2 exerted distinct inhibitory effects on expression of CCAAT/enhancer binding protein alpha (C/EBPα) and peroxisome proliferatoractivated receptor gamma (PPARγ), which are two key transcriptional factors regulating the expression of adipogenic genes. CONCLUSION Decrease of AD expression in adipocytes by ROS involves the decrease of C/EBPα and PPARγ.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期:2007-05-28.通讯作者:于军,讲师,博士,主要从事心血管疾病的研究 Email: yujunby@fmmu.edu.cn 作者简介:陈宝莹,主治医师,博士Email:chenby128@yahoo.com
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