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牛磺酸对低温保存大鼠心脏的心肌形态和漏出酶的影响(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2008年第2期
页码:
147-149
栏目:
基础研究
出版日期:
2008-03-20

文章信息/Info

Title:
Effects of taurine on enzyme leakage and morphology of heart in hypothermal preservation
作者:
孙长滨1;  侯芝奇1;  刘萌萌1;  都枰君1;  迟庆2; 周士胜2
大连大学医学院:1.组织学与胚胎学教研室，2.医学实验中心，辽宁 大连 116622
Author(s):
SUN Changbin1;  HOU Zhiqi1; LIU Mengmeng1;  DOU Pingjun1;  CHI Qing2;  ZHOU Shisheng2
1.Department of Histology and Embryology, 2.Center of Medical Laboratory, Medical College of Dalian University, Dalian 116622, Liaoning, China
关键词:
心脏;  器官保存;  牛磺酸
Keywords:
heart;  organ preservation;  taurine
分类号:
R318.11
DOI:
-
文献标识码:
A
摘要:
目的 研究牛磺酸在心肌低温冷缺血损伤中的保护作用。方法 SD大鼠随机分成2组，包括对照组（HTK液保存），牛磺酸组（HTK液中加15 mmol/L牛磺酸）。利用离体鼠心非循环式Langendorff 灌注模式装置，经4℃保存4h后，观察复温再灌时的冠脉漏出液中心肌酶的漏出量，并切取心肌组织做光镜和电镜观察。结果 与对照组比较，牛磺酸组能减少心肌酶（乳酸脱氢酶、磷酸肌酸激酶及谷草转氨酶）的漏出，减轻线粒体的损伤。结论 牛磺酸对心肌的冷缺血损伤具有保护作用。
Abstract:
AIM To investigate the effects of taurine on heart hypothermal preservation. METHODS Twenty SD rats were divided randomly into 2 groups: Control group, in which hearts were arrested and stored in HTK solution and Taurine group, in which hearts were arrested and stored in HTK solution containing taurine (15 mmol/L). Langendorff model perfusion apparatus of isolated rat heart was used. The isolated rat hearts were stored in 4℃ in different preservation solutions for 4 hours respectively, and then were reperfused. After 4 minutes' reperfusion, the leakage of myocardial enzyme in coronary effluent was determined. After 10 minutes' reperfusion, the heart samples were obtained and observed under lighter and electronic microscopes. RESULTS Compared with that in control group, the leakage of myocardial enzymes (lactate dehydrogenase, creatine kinase and glutamatoxalacetat transaminase) in the coronary effluent was significantly reduced and the damage of mitochondria was slight in the taurine group. CONCLUSIONT aurine can protect the myocardium in the heart hypothermal preservation.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2007-10-08.通讯作者：周士胜，教授，主要从事心肌细胞信号传导和低温 对心肌功能影响研究Email:zhouss@dlu.edu.cn 作者简介：孙长滨，副教授，博士Email:scb5711@126.com
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