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雷公藤内酯醇涂层支架对猪冠状动脉内皮结构和再狭窄的影响(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2008年第5期
页码:
525-528
栏目:
基础研究
出版日期:
2008-10-20

文章信息/Info

Title:
Effect of triptolide coated stent on endothelial structure and restenosis in porcine coronary
作者:
葛海龙1; 周玉杰1; 马涵英1; 刘蕾2; 聂小敏1; 闫阵娴1; 聂滨1; 贾德安1
1.首都医科大学附属北京安贞医院心内科 北京心肺血管疾病研究所,北京 100029; 2.中国医学科学院阜外心血管病医院病理科，北京 100029
Author(s):
GE Hailong;  ZHOU Yujie;  MA Hanying;  LIU Lei;  NIE Xiaomin;  YAN Zhenxian;  NIE Bin;  JIA Dean
Department of Cardiology, Anzhen Hospital, Capital University of Medical Science, Beijing 100029, China
关键词:
冠状动脉血管成形术; 经腔; 经皮; 内皮; 支架
Keywords:
coronary angioplasfy;  transluminal;  percutaneous;  endothelium;  stent
分类号:
R543.3
DOI:
-
文献标识码:
A
摘要:
目的 观察雷公藤内酯醇涂层支架（triptolide）置入猪冠状动脉后,血管内膜增殖过程中NF-κB、ki67表达的变化,探讨该药物支架对血管内皮的影响。方法 将8只猪进行冠状动脉支架置入术,将雷公藤内酯醇涂层支架和裸支架组对照，置入前降支、回旋支、右冠脉近端。术后12周处死动物,采用光镜观察内膜增生情况,扫描电镜观察内皮修复情况,免疫组织化学法检测平滑肌细胞NFκB、ki67表达。结果 术后12周,triptolide组血管内膜增生［(内膜厚度（0.12±0.05）mm］、内膜面积［（1.17±025)mm2 ］明显小于对照组［内膜厚度［(0.35±011)mm,P<0.01］,内膜面积［（1.81±0.36）mm2,P<0.05］;电镜显示血管内膜表面的内皮细胞较对照组分布更紧密,排列更整齐,覆盖更完整;药物组NF-κB、ki67表达量显著低于对照组。结论 雷公藤内酯醇涂层支架可抑制猪冠状动脉平滑肌细胞增殖，有效控制局部炎性活动，抑制内膜增殖。
Abstract:
AIM To investigate the effect of stent coated with triptolide on the expression of NFκB and ki67 on VSMC proliferation in porcine. METHODS Eight pigs were randomly divided into 2 groups to undergo implantation of stents coated with triptolide (TES) or bare metal stents (BMS) into the proximal of left anterior coronary, left circumflex coronary and right coronary. Twenty weeks after the implantation, the pigs were killed and their coronary arteries with the stents inside were taken out and examined by light microscopy, scanning electron microscopy for neointimal hyperplasia, and immunohistochemistry for the expression of NFκB and ki67. RESULTS The thickness of neointimal in BMS was ［（0.56±0.08）mm］, significantly thicker than that of the other group ［(0.23±0.07)mm, P<0.05］. The area of neointimal in BMS was ［（2.72±0.32）mm2］, significantly larger than that of the other group ［(1.36±026)mm2, P<0.01］. Immnmunohistochemistry showed that the expression of NF-κB and ki67 in BMS group was 5.54±0.23 and 12.7±3.2, significantly higher than that in TES group (2.44±0.16 and 9.5±1.3, P<0.05). CONCLUSION Triptolideeluting stents can prevent restenosis through inhibiting neointimal proliferation and inflammation within 12 weeks.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2008-02-21.基金项目：首都医学发展科学基金项目资助（03Ⅱ09）；北京市中医药科技项目资助（JJ200327） 通讯作者：周玉杰，教授，主要从事介入心脏病学研究Email:azzyj12@163.com 作者简介：葛海龙，主治医师，博士Email:gehailong@126.com
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