我们的网站为什么显示成这样？

可能因为您的浏览器不支持样式，您可以更新您的浏览器到最新版本，以获取对此功能的支持，访问下面的网站，获取关于浏览器的信息：

|本期目录/Table of Contents|

超速起搏预适应的延迟保护与热休克蛋白70的表达(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2008年第6期
页码:
678-681
栏目:
基础研究
出版日期:
2008-12-25

文章信息/Info

Title:
Delayed cardioprotection induced by ventricular overdrive pacing preconditioning and expression of heat shock protein 70
作者:
吴立春1; 陈国桢2; 方中良1
1.岳阳市第二人民医院ICU科，湖南 岳阳 414000； 2.福建医科大学附属第二医院心内科，福建 泉州 362000
Author(s):
U Lichun1;  CHEN Guozhen2;  FANG Zhongliang1
1.Department of ICU, Second Municipal People’s Hospital of Yueyang, Yueyang 414000, Hunan, China; 2.Department of Cardiology, Second Affiliated Hospital of Fujian Metical University, Quanzhou 362000, Fujian, China
关键词:
超速心室起搏; 预适应; 热休克蛋白; 延迟保护作用
Keywords:
ventricular overdrive pacing;  preconditioning;  heat shock protein;  delayed cardioprotection
分类号:
R541.4
DOI:
-
文献标识码:
A
摘要:
目的 观察在超速心室起搏(ventricular overdrive pacing,VOP)预适应延迟保护阶段热休克蛋白70(HSP70)的表达水平。方法 新西兰兔24只，随机分为3组，单纯结扎组，起搏组，起搏+放线菌素D组。制作超速起搏预适应和缺血/再灌注的动物模型，检测CK和CKMB的变化，动态描记再灌注时心电图，免疫组织化染色检测HSP70抗原。结果 缺血后起搏组心肌酶的水平在再灌注时均低于单纯结扎组和起搏+放线菌素D组（P<0.01）；单纯结扎组中在再灌注过程中共有5只发生心律失常，起搏+放线菌素组也有4只，而起搏组无心律失常发生。起搏组和单纯结扎组之间有显著性差别(P<0.05)。起搏组HSP70的阳性表达的程度明显高于其他两组。结论 超速起搏预适应可以模拟缺血预适应，其延迟保护作用可能与HSP70表达增加密切相关。
Abstract:
AIM To observe the expression of heat shock protein 70 (HSP70) in late phase of cardioprotection induced by ventricular overdrive pacing preconditioning. METHODS Twentyfour New Zealand rabbits were randomly divided into 3 groups: the simple ligation group, the pacing group and the pacing+actinomycin group. The animal models of ventricular overdrive pacing preconditioning and ischemia and reperfusion were made. The levels of creatine kinase (CK) and creatinine kinase isoenzyme (CKMB) were monitored and the electrocardiogram was recorded during the phase of reperfusion. HSP70 was tested by immunohistochemistry. RESULTS The levels of CK and CKMB were significantly lower in the pacing group when compared with those in the simple ligation group and the pacing+actinomycin group at the different phases after ischemia（P<0.01）. Arrhythmia was found in 5 cases in the simple ligation group and 4 cases in the pacing+actinomycin group, but no one in the pacing group. The occurrence rate of arrhythmia was significantly different between the simple ligation group and the pacing group(P<0.05). The levels of HSP70 expression in the pacing group were significantly higher than those in the other two groups. CONCLUSION The overdrive pacing preconditioning can mimic the ischemic preconditioning and the delayed cardioprotection may closely correlated with the overexpression of HSP70.

参考文献/References

［1］ Rizvi A, Tang XL, Qiu Y, et al. Increased protein synthesis is necessary for the development of late preconditioning against myocardial stunning［J］. Am J Physiol, 1999, 277(3 Pt 2):H874-H884.

［2］ 李志高，李晓冬，董新舒，等. 大肠癌组织RAB5A和CD44v9表达的意义［J］. 世界华人消化杂志, 2006, 14 (9):848-852.

［3］ Lazou A, Iliodromitis EK, Cieslak D, et al. Ischemic but not mechanical preconditioning attenuates ischemia/reperfusion induced myocardial apoptosis in anaesthetized rabbits: The role of Bcl2 family proteins and ERK1/2［J］. Apoptosis, 2006, 11(12):2195-2204.

［4］ 吴棘，李治安，何云. 快速起搏预适应减少缺血再灌注所致心律失常的实验研究［J］. 广西医科大学学报, 2004, 21(1):39-41.

［5］ Driamov SV, Bellahcene M, Butz S, et al. Bradykinin is a mediator, but unlikely a trigger, of antiarrhythmic effects of ischemic preconditioning［J］. J Cardiovasc Electrophysiol, 2007, 18(1):93-99.

［6］Guisasola MC, Desco Mdel M, Gonzalez FS, et al. Heat shock proteins, end effectors of myocardium ischemic preconditioning?［J］. Cell Stress Chaperones, 2006 ,11(3):250-258.

［7］ Liu J, Kam KW, Borchert GH, et al. Further study on the role of HSP70 on Ca2+ homeostasis in rat ventricular myocytes subjected to simulated ischemia［J］. Am J Physiol Cell Physiol, 2006, 290(2):583-591.

［8］ Liu J, Kam KW, Zhou JJ, et al. Effects of heat shock protein 70 activation by metabolic inhibition preconditioning or kappaopioid receptor stimulation on Ca2+ homeostasis in rat ventricular myocytes subjected to ischemic insults［J］. J Pharmacol Exp Ther, 2004, 310(2):606-613.

［9］Hampton CR, Shimamoto A, Rothnie CL, et al. HSP70.1 and 70.3 are required for latephase protection induced by ischemic preconditioning of mouse hearts［J］. Am J Physiol, 2003, 285(2):H866-H874.

［10］Qian YZ, Bernardo NL, Nayeem MA, et al. Induction of 72kDa heat shock protein does not produce second window of ischemic preconditioning in rat heart［J］. Am J Physiol, 1999, 276(1 Pt 2):H224-H234.

［11］谢强，黄从新，吴钢，等. 超速起搏预处理对急性心肌梗死的延迟保护作用［J］. 中华急诊医学杂志, 2004, 13(1):30-33.

备注/Memo

备注/Memo:
收稿日期：2007-09-10.作者简介：吴立春，医师，硕士Email:spring036@126.com
更新日期/Last Update: 2009-03-24