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恒定磁场对大鼠心肌缺血/再灌注损伤的影响(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2008年第6期
页码:
694-696
栏目:
基础研究
出版日期:
2008-12-25

文章信息/Info

Title:
Effect of constant magnetic field on myocardial ischemiareperfusion injury in rats
作者:
李敬霞; 程何祥; 牟翔; 张荣庆; 栾荣华; 王海昌
第四军医大学西京医院心血管内科，陕西 西安 710032
Author(s):
LI Jingxia;  CHENG Hexiang;  MU Xiang;  ZHANG Rongqing;  LUAN Ronghua;  WANG Haichang
Department of Cardiology, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, Shaanxi, China
关键词:
恒定磁场; 心肌; 缺血/再灌注损伤; 细胞凋亡
Keywords:
constant magnetic field;  myocardium;  ischemia/reperfusion injury;  cell apoptosis
分类号:
R542.4
DOI:
-
文献标识码:
A
摘要:
目的 观察恒定磁场干预对大鼠心肌缺血/再灌注(I/R)损伤的影响。方法 雄性SD大鼠24只，随机分为4组，每组6只，即假手术组、I/R组、50 mT恒定磁场+I/R组及100 mT恒定磁场+I/R组。大鼠麻醉开胸，结扎冠状动脉前降支40 min，再灌注4 h。采用Evan’s蓝和TTC染色法测定心肌梗死范围；用TUNEL法测定大鼠心肌组织细胞的凋亡；采用全自动生化分析仪测定血清肌酸激酶(CK)的水平，采用微粒子化学发光法测定血清肌钙蛋白I(cTnI)的水平。结果 50 mT及100 mT 恒定磁场干预组I/R后，心肌梗死面积和细胞凋亡指数均显著小于I/R组(P<0.05)。I/R组大鼠血清CK和cTnI的水平与假手术组比较显著升高(P<0.05)；而不同强度的恒定磁场干预+I/R组血清CK和cTnI的水平与I/R组比较显著降低(P<0.05)。结论 恒定磁场干预能有效地抑制I/R所致大鼠心肌的损伤。
Abstract:
AIM To investigate the effect of constant magnetic field (CMF) on myocardial ischemia/reperfusion injury (IRI) in rats. METHODS Twentyfour male SpragueDawley rats were randomly divided into four groups, 6 rats each group, sham operation group, IRI group, 50 mT CMF intervention group and 100 mT CMF intervention group. The rats were anesthetized and then underwent 40 min of myocardial ischemia and 4 h of reperfusion. The infarct size was assessed by Evans’ blue and TTC staining. Cardiomyocyte apoptosis was determined by TUNEL method and the level of serum cardiac troponin I (cTn I) was detected by chemiluminescent enzyme immunoassay. The level of sera creatine kinase (CK) was determined by automatic biochemistry analyzer. RESULTS The ratio of infarct size to area at risk (IS/AAR) and cardiomyocyte apoptosis indexes decreased following intervention with 50 mT or 100 mT CMF, which were significantly smaller than those in IRI group (P<0.05). The levels of serum CK and cTnI in IRI group were significantly higher than those in sham operation group (P<0.05), whereas the levels of serum CK and cTnI in 50 mT or 100 mT CMF intervention group were significantly lower than those in IRI group (P<0.05). CONCLUSION Intervention with CMF protects the heart against IRI.

参考文献/References

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［3］ Zhao ZQ, Morris CD, Budde JM, et al. Inhibition of myocardial apoptosis reduces infarct size and improves regional contractile dysfunction during reperfusion［J］. Cardiovasc Res, 2003, 59(1)：132-142.

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［9］ Gmitrov J, Ohkubo C, Okano H. Effect of 025 T static magnetic field on microcirculation in rabbits［J］. Bioelectromagnetics, 2002, 23(3):224-229.

备注/Memo

备注/Memo:
收稿日期：2007-12-19.基金项目：第四军医大学西京医院2005年临床高新技术项目资助（XJGX0541H03） 通讯作者：王海昌，教授，主要从事冠心病的基础与临床研究Email:Wanghc @fmmu.edu.cn 作者简介：李敬霞，副主任护师Email:Lijx6556 @fmmu.edu.cn
更新日期/Last Update: 2009-03-24