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|本期目录/Table of Contents|

快速起搏心房肌细胞中MAPKs表达的变化

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2009年第3期
页码:
300-303
栏目:
基础研究
出版日期:
2009-05-15

文章信息/Info

Title:
Expressions of MAPKs in rapidly paced atrial myocytes
作者:
程伟; 肖颖彬
第三军医大学新桥医院全军心血管外科中心,重庆 400037
Author(s):
CHENG Wei;  XIAO Ying-bin
Department of Cardiovascular Surgery, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China
关键词:
心房纤颤; 丝裂原激活蛋白激酶; 快速起搏; 重建; 心房肌细胞
Keywords:
atrial fibrillation;  MAPKs;  rapid pacing;  remodeling;  atrial myocytes
分类号:
R977.2；R541.7
DOI:
-
文献标识码:
A
摘要:
目的 研究快速起搏后心房肌细胞超微结构和丝裂原激活蛋白激酶（MAPKs）表达的变化。方法 原代培养大鼠的心房肌细胞，并建立快速起搏细胞模型。实验分为对照组和快速起搏组，采用Western-blot检测细胞外信号调节激酶（ERK）和p38MAPK的蛋白表达及其磷酸化水平的变化；用透射电镜观察快速起搏24 h后心房肌细胞超微结构的变化。结果 快速起搏24 h后，ERK和磷酸化ERK的表达均较起搏前明显升高(P<0.01)；p38MAPK在快速起搏前后的表达无明显变化，而磷酸化p38MAPK的表达则较起搏前明显升高(P<0.01)。经过24 h的快速起搏，心房肌细胞出现糖原聚集、核固缩、空泡样变以及肌浆网扩张、线粒体肿胀、部分线粒体嵴轻度溶解等超微结构的改变。结论 快速起搏早期，原代培养的心房肌细胞超微结构发生了明显改变，同时ERK和p38MAPK被激活，这可能是心房纤颤早期心房肌细胞结构重建发生的重要机制。
Abstract:
AIM To study the changes of ultramicrostructure in atrial myocytes and the expressions of mitogen-activated protein kinases (MAPKs) after rapid pacing. METHODS Rat primary atrial myocytes were cultured and a rapidly paced cell model was established. The atrial myocytes were divided into rapid pacing group and control group. Western blot was employed to detect the changes of protein expressions of extra-cellular signal regulation kinase (ERK), p38MAPK and their phosphorylation level, respectively. The ultramicrostructure in atrial myocytes was observed under transmission electromicroscope at 24 hours after rapid pacing. RESULTS Expressions of ERK, p-ERK and p-p38MAPK were all upregulated at 24 hours after rapid pacing, compared to those before pacing （P<0.01）, however, no significant change was observed in the expression of p38MAPK. The ultramicrostructure in atrial myocytes displayed glycogen aggregation, karyopycnosis, sarcoplasmic reticulum distention and chondriosome swelling at 24 hours after rapid pacing. CONCLUSION Obvious change of ultramicrostructure in primarily atrial myocytes takes place in the early phase of rapid pacing and at the same time ERK and p38MAPK are activated. It may be an important mechanism of structure remodeling of atrial myocytes in the early of atrial fibrillation.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2007-7-12.基金项目：国家自然科学基金项目资助(30600250) 通讯作者：肖颖彬，主任医师，主要从事心房纤颤方面的研究Email:xiaoyb607@163.com 作者简介：程伟，主治医师，博士Email:yjchw@126.com
更新日期/Last Update: 2009-05-18