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iNOS及ERK1/2信号转导通路在17β-雌二醇抑制血管平滑肌细胞增殖中的作用

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2009年第6期
页码:
761-764,773
栏目:
基础研究
出版日期:
2009-11-05

文章信息/Info

Title:
Effect of iNOS and ERK1/2 on 17β-estradiol-induced inhibition of vascular smooth muscle cell proliferation
作者:
刘海梅1; 赵晓峰2; 林桂平3; 徐进文3; 王庭槐3
1.广州中医药大学生理学教研室，广东 广州 510006；2.韶关学院医学院生理学教研室， 广东 韶关 512026；3.中山大学中山医学院生理学教研室，广东 广州 510080
Author(s):
LIU Hai-mei1;  ZHAO Xiao-feng2;  Lin Gui-ping3;  XU Jin-wen3;  WANG Ting-huai3
1.Department of Physiology, Guangzhou University of Chinese Medicine, Guangzhou 510006, Guangdong, China; 2.Department of Physiology, Medical College of Shaoguan University, Shaoguan 512026, Guangdong, China; 3.Department of Physiology, Zhongshan Medical College, Sun-Yat Sen University, Guangzhou 510080, Guangdong, China
关键词:
17β-雌二醇; 诱导型一氧化氮合酶; 细胞外信号调节激酶1/2; 血管平滑肌细胞
Keywords:
17β-estradiol;  iNOS;  ERK1/2;  vascular smooth muscle cell
分类号:
Q579.13
DOI:
-
文献标识码:
A
摘要:
目的: 观察诱导型一氧化氮合酶（inducible nitric oxide synthase，iNOS）及细胞外信号调节激酶1/2（ERK1/2）信号转导通路在17β-雌二醇（17β-estradiol，E2）抑制血管平滑肌细胞（vascular smooth muscle cells，VSMCs）增殖中的作用。方法: 采用MTT比色法和Western blot技术，检测E2预处理前后胎牛血清（fetal calf serum，FCS）对VSMCs中DNA合成、iNOS表达及磷酸化的ERK1/2蛋白表达的影响。结果: E2作用24 h，可明显抑制FCS诱导的VSMCs增殖。Western blot的结果显示，FCS孵育VSMCs后，iNOS蛋白的表达下降，磷酸化的ERK1/2蛋白表达增加。E2预处理后，可增加iNOS蛋白的表达，抑制磷酸化ERK1/2蛋白表达。若提前应用iNOS阻断剂L-精氨酸甲酯（L-NAME）孵育VSMCs，可部分逆转E2诱导的磷酸化的ERK1/2蛋白表达下降的效应。结论: E2可抑制FCS诱导的VSMCs增殖，其作用可能与增加iNOS蛋白的表达，抑制磷酸化的ERK1/2表达有关。
Abstract:
AIM: To investigate the effect of iNOS and phosphorylation of ERK1/2 on 17β-estradiol (E2)-induced inhibition of vascular smooth muscle cells (VSMCs). METHODS: In cultured VSMCs, proliferation was determined by MTT assay, and the expression of iNOS and ERK1/2 phosphorylation were measured by Western blot. RESULTS: The measurement showed that exposure to fetal calf serum (FCS) for 24 h increased the proliferation of VSMCs. Pretreatment with E2 for 24 h inhibited VSMC proliferation under growth-stimulating (10% FCS) condition. Western blot showed that FCS inhibited expression of iNOS and increased expression of ERK1/2 phosphorylation in VSMCs. Pretreatment with E2 for 24 h partly reversed this effect. Western blot results further proved that pretreatment with iNOS inhibitor, L-NAME, partly reversed the increase of ERK1/2 phosphorylation induced by FCS. CONCLUSION: Our results demonstrate that 17β-estradiol increases iNOS expression and decreases ERK1/2 phosphorylation, leading to inhibition of VSMC proliferation.

参考文献/References

［1］ Pozo M, Izquierdo MC, de Nicolás R, et al. Gliotoxin Inhibits Neointimal Hyperplasia after Vascular Injury in Rats［J］. J Vasc Res, 2008, 46(4):278-289.

［2］ Karpurapu M, Wang D, Singh NK, et al. NFATc1 targets cyclin A in the regulation of vascular smooth muscle cell multiplication during restenosis［J］. J Biol Chem, 2008, 283(39):26577-26590.

［3］ Kappert K, Caglayan E, Huntgeburth M, et al. 17Beta-estradiol attenuates PDGF signaling in vascular smooth muscle cells at the postreceptor level［J］. Am J Physiol Heart Circ Physiol, 2006, 290(2):H538-H546.

［4］ Ba ZF, Lu A, Shimizu T, et al. 17beta-Estradiol modulates vasoconstriction induced by endothelin-1 following trauma-hemorrhage ［J］. Am J Physiol Heart Circ Physiol, 2007, 292(1):H245-H250.

［5］ Yan ZQ, Yokota T, Zhang W, et al. Expression of inducible nitric oxide synthase inhibits platelet adhesion and restores blood flow in the injured artery［J］. Circ Res, 1996, 79(1):38-44.

［6］ Rodríguez A, Fortuo A, Gómez-Ambrosi J, et al. The inhibitory effect of leptin on angiotensin II-induced vasoconstriction in vascular smooth muscle cells is mediated via a nitric oxide-dependent mechanism［J］. Endocrinology, 2007, 148(1):324-331.

［7］ Wang Y, Yan T, Wang Q, et al. PKC-dependent extracellular signal-regulated kinase 1/2 pathway is involved in the inhibition of Ib on AngiotensinII-induced proliferation of vascular smooth muscle cells［J］. Biochem Biophys Res Commun, 2008, 375(1):151-155.

［8］ Abe K, Nakashima H, Ishida M, et al. Angiotensin II-induced osteopontin expression in vascular smooth muscle cells involves Gq/11, Ras, ERK, Src and Ets-1［J］. Hypertens Res, 2008, 31(5):987-998.

备注/Memo

备注/Memo:
收稿日期：2008-12-18.基金项目：国家自然科学基金项目资助（30440028） 通讯作者：王庭槐，教授，主要从事心血管疾病的实验研究及生物反馈研究Email:wangth@mail.sysu.edu.cn 作者简介：刘海梅，讲师，博士Email：happyliuhm@yahoo.com.cn
更新日期/Last Update: 2009-09-30