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高氧液预处理对糖尿病兔心肌缺血/再灌注时血浆因子及冠状动脉张力的影响

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2009年第6期
页码:
770-773
栏目:
基础研究
出版日期:
2009-11-05

文章信息/Info

Title:
Protective effects of hyperoxia pretreatment on coronary artery against ischemia/reperfusion injury in diabetic rabbits
作者:
徐瑞芬1; 冯旭阳2; 刘蕊3; 张惠1; 徐礼鲜1; 高巍1
第四军医大学：1.口腔医院麻醉科，2.西京医院心脏内科，3.口腔医院颌面外科，陕西 西安 710032
Author(s):
XU Rui-fen;  FENG Xu-yang;  LIU Rui;  ZHANG Hui;  XU Li-xian;  GAO Wei
Department of Anesthesiology, College of Stomatology, Fourth Military Medical University, Xi’an, Shaanxi 710032, China
关键词:
高氧液; 糖尿病; 心肌再灌注损伤; 冠状动脉
Keywords:
hyperoxia solution;  diabetes mellitus;  myocardial reperfusion injury;  coronary vessels
分类号:
R459.6
DOI:
-
文献标识码:
A
摘要:
目的: 探讨预先给予不同剂量的高氧液（HO）对糖尿病兔心肌缺血/再灌注(I/R)时冠状动脉的影响。方法: 以32只家兔建立糖尿病模型后喂养8周，随机分为4组（n=8）, 即假手术组（Sham组）：只穿线环绕冠状动脉左前支(LAD)不结扎；吸氧组（OX组）：于结扎前30 min，经鼻吸纯氧2 L/min；HO1组：在结扎LAD前30 min，静脉注射HO液10 ml/kg；HO2组：结扎前30 min，静注HO液20 ml/kg。于结扎LAD前即刻（T1,基础值）、开放LAD前即刻（T2）及开放后120 min（T3）时，颈总静脉插管取血，测定血清一氧化氮(NO)、内皮素(ET)-1和血栓素B2(TXB2)的浓度，计算冠状动脉灌注压(CPP)。取LAD，测定其在硝普钠(SNP)和去甲肾上腺素（NE)作用下的张力。结果: T2,3时，Sham组CPP的恢复率高于其余3组；HO2组CPP的恢复率高于OX组和HO1组（P<0.05）。与T2时相比，T3时各组CPP的恢复率明显降低（P<0.05）；与T1时相比较，T2,3时OX组血清NO的浓度降低，OX组、HO1组和HO2组TXB2的浓度升高；T2时OX组、HO1组血清ET-1的浓度升高，T3时OX组血清ET-1的浓度升高（P<0.05）。与OX组相比，T2,3时HO2组血清NO的浓度明显升高，ET-1和TXB2的浓度明显降低（P<0.05）。与Sham组相比，在NE作用下其余各组冠状动脉收缩张力均降低。在SNP作用下，OX组和HO1组冠状动脉舒张张力升高，HO2组降低（P<0.05）；HO1组和HO2组冠脉收缩张力高于OX组，HO2组冠脉收缩张力高于HO1组（P<0.05）。结论: 预先静脉注射HO液对糖尿病兔心肌I/R时的冠状动脉具有一定的保护作用，注射20 ml/kg HO液的效果优于注射10 ml/kg HO液。
Abstract:
AIM: To investigate the protective effects of different doses of hyperoxia pretreatment on the coronary artery against ischemia/reperfusion (I/R) injury in diabetic rabbits. METHODS: Adult male diabetic rabbits weighing 2-3 kg were used in this study. Diabetes mellitus (DM) was induced with IV alloxan (120 mg/kg). Eight weeks later, the animals were randomly divided into four groups (n=8 each): group S (sham operation), group OX (myocardial I/R), group HO1 and group HO2, which received hyperoxia solution (10 or 20 ml/kg), respectively, at 30 min before myocardial ischemia. A catheter was inserted into the left ventricle via the left common carotid artery for determination of left ventricular end-diastolic pressure (LVEDP), and right femoral artery was cannulated for BP monitoring. Myocardial I/R was produced by temporary ligation of the anterior descending branch of the left coronary artery (LAD) for 60 min followed by 120-min reperfusion. Myocardial ischemia was confirmed by S-T segment elevation and changes in color of myocardium. Blood samples were obtained immediately before ligation of LAD (T1), immediately before LAD ligation was untied (T2) and 120 min after LAD was untied (T3) for determination of serum NO, ET-1 and TXB2 concentrations and concentrations and coronary perfusion pressure (CPP) (CPP=LVEDP-DBP). Animals were then sacrificed and LAD was isolated. Vasomotor reaction to norepinephrine (NE) and sodium nitroprusside (SNP) was examined. RESULTS: The recovery rate of CPP was significantly better at T2 and T3 in HO2 group than in OX and HO1 groups. Serum NO concentration significantly decreased, whereas serum ET-1 and TXB2 concentration significantly increased at T2 and T3 compared to baseline values at T1 in group I/R (OX group). Serum NO concentration was significantly higher, whereas serum ET-1 and TXB2 concentration were significantly lower at T2 and T3 in HO2 group than those in OX group. Vascular tension was significantly higher in the presence of NE and lower in the presence of SNP in HO2 group compared with those in OX and HO1 groups. CONCLUSION: Pretreatment with hyperoxia solution protects the coronary artery from I/R injury in diabetic rabbits. Hyperoxia solution of 20 ml/kg has demonstrated a better protective effect than hyperoxia solution of 10 ml/kg.

参考文献/References

［1］ Prior JO, Quinones MJ, Hernandez-Pampaloni M, et al. Coronary circulatory dysfunction in insulin resistance, impaired glucose tolerance, and type 2 diabetes mellitus［J］. Circulation, 2005, 111(18):2291-2298.

［2］ Gao C, Xu L, Chai W, et al. Amelioration of intestinal ischemia reperfusion injury with intraluminal hyper-oxygenated solution: studies on structural and functional changes of enterocyte mitochondria［J］. J Surg Res, 2005, 129(2):298-305.

［3］ 徐叔云，卞如濂，陈修,等. 药理实验方法学［M］. 北京： 人民卫生出版社，1982.

［4］ Mahgoub MA, Abd-Elfattah AS. Diabetes mellitus and cardiac function［J］. Mol Cell Biochem, 1998,180(1-2):59-64.

［5］ 吴清玉. 冠状动脉外科学［M］. 北京: 人民卫生出版社， 2003.

［6］ Verma S, Maitland A, Weisel RD, et al. Increased endothelin-1 production in diabetic patients after cardioplegic arrest and reperfusion impairs coronary artery vascular teactivity: reversal by means of endothelin antagonism［J］. J Thorac Caediovasc Surg, 2002, 123(6):1114-1119.

［7］ Verma S, Maitland A, Weisel RD, et al. Hyperglycemia exaggerates schemia-reperfusion-induced cardiomyocyte injury: reversal with endothelin antagonism［J］. J Thorac Caediovasc Surg, 2002, 123(6):1120-1124.

［8］ Xu RF, Li TT, Feng XY, et al. Therapeutic effect of hyperoxygenated solution on acute lung injury induced by oleid acid［J］. Eur Surg Res, 2008, 41(1):37-43.

备注/Memo

备注/Memo:
收稿日期：2008-12-11.基金项目：全军十一五科技攻关项目资助（06G093） 通讯作者：徐礼鲜，教授，主任医师，主要从事高氧液及全麻机理的研究Email:kqmzk@fmmu.edu.cn 作者简介：徐瑞芬，主治医师，博士生Email:xuruifen @fmmu.edu.cn
更新日期/Last Update: 2009-09-30