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|本期目录/Table of Contents|

去甲肾上腺素预处理晚期出现的线粒体跨膜电位的变化

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2009年第6期
页码:
779-781
栏目:
基础研究
出版日期:
2009-11-05

文章信息/Info

Title:
Change of transmembrane potential during delayed phase of noradrenaline preconditioning
作者:
朱元州; 冯义柏; 常超; 王裕勤
武汉科技大学附属医院心内科，湖北 武汉 430064
Author(s):
ZHU Yuan-zhou;  FENG Yi-bai;  CHANG Chao;  WANG Yu-qin
Department of Cardiology, Affiliated Hospital, Wuhan University of Science and Technology, Wuhan 430064, Hubei, China
关键词:
去甲肾上腺素; 预适应; 线粒体; 跨膜电位
Keywords:
noradrenaline;  preconditioning;  mitochondria;  transmembrane potential
分类号:
R971.93
DOI:
-
文献标识码:
A
摘要:
目的: 研究微量去甲肾上腺素(NA)预处理晚期心肌细胞线粒体跨膜电位的变化，为探讨预适应晚期作用机制提供依据。方法: 原代培养乳鼠心肌细胞随机分为3组，即对照（control,CON）组，缺氧处理（hypoxia treatment,HT）组和NA预处理 (noradrenaline preconditioning,NAPC)组。CON组：不给任何药物干预，也不进行HT，培养24 h；HT组：不给予任何药物干预，培养24 h后直接进行HT 40 min；NAPC组：预先给予终浓度为0.2 mg/L的NA孵育10 min后，继续培养24 h再进行HT 40 min。用流式细胞仪检测各组罗丹明123（rhodamine 123）标记的线粒体的荧光强度（fluorescence intensity,FI）。结果: 3组的FI值通过方差分析比较（F=355.5,P<0.05），HT组与NAPC组的FI比CON组明显降低［（6.24±0.13）%、（6.68±0.22）% vs （8.32±0.19）%,P<0.05）］，表明HT组与NAPC组心肌细胞线粒体的跨膜电位明显降低，细胞能量代谢能力下降；NAPC组FI比HT组高［(6.68±0.22)% vs （6.24±0.13）%，P<0.05)］，表明NAPC组心肌细胞线粒体的跨膜电位较高，NA预处理过的细胞能量代谢能力较强。结论: NAPC组的心肌细胞线粒体的FI较HT组明显升高，表明NA预处理晚时期跨膜电位降较高，NA触发了细胞保护效应，使心肌细胞对损伤性刺激的耐受力增强。
Abstract:
AIM: To investigate the effect of noradrenaline (NA) on transmembrane potential during delayed preconditioning phase. METHODS: Primary culture myocardial cells of neonatal rat were randomly divided into three groups, namely, control (CON) group, hypoxia treatment (HT) group, and NA preconditioning (NAPC) group. CON group was cultured for 24 h without any drug interference, HT group was cultured for 24 h without any drug interference but then treated for 40 min with hypoxia, and NAPC group was exposed to NA (0.2 mg/L) for 10 min and then treated with hypoxia for 40 min after the 24-h culture. Fluorescence intensity (FI) of rhodamine-123-marked mitochondria was detected by flow cytometry. RESULTS: FI of the three groups was examined by analysis of variance (F=355.5, P<0.05). FIs of HT group and NAPC group were lower than that of CON group ［(6.24±0.13)%, (6.68±0.22)% vs. (8.32±0.19)%, P<0.05］. Transmembrane potentials of HT group and NAPC group were lower than that of CON group, indicating that the energy synthesis was defective. FI of NAPC group was higher than HT group and the transmembrane potential of NAPC group was higher than HT group, indicating that NA increased energy synthesis and improved cardiomyocyte metabolism. CONCLUSION: FI of NAPC group is higher than HT group, which indicates that NA improves the transmembrane potential.

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备注/Memo

备注/Memo:
收稿日期：2008-7-31.通讯作者：冯义柏，教授，主要从事心肌缺血与电生理研究Email:Yibaifeng688@hotmail.com 作者简介：朱元州，主治医师，硕士Email:yuanzhou.z@hotmail.com
更新日期/Last Update: 2009-09-30