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人骨髓间充质干细胞在心肌损伤裸鼠体内的归巢与分化

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2009年第6期
页码:
786-789
栏目:
基础研究
出版日期:
2009-11-05

文章信息/Info

Title:
Homing and differentiation of human bone marrow mesenchymal stem cells in myocardial damage
作者:
邓方阁1; 2; 张秀英1; 王芯蕊3; 马英智1; 李玉林1
吉林大学:1.白求恩医学部病理生物学教育部国家重点实验室, 吉林 长春 130021, 3.人兽共患病研究所人兽共患病教育部国家重点实验室，吉林 长春 130062;2.广州 医学院第一附属医院广州呼吸疾病研究所呼吸疾病国家重点实验室，广东 广州510120
Author(s):
DENG Fang-ge1; 2;  ZHANG Xiu-ying1;  WANG Xin-rui3;  MA Ying-zhi1;  LI Yu-lin1
1.ME Key Laboratory of Pathobiology, Norman Bethune College of Medicine, Jilin University, Changchun 130021, Jilin, Chine; 2.National Key Lab of Respiratory Disease, Guangzhou Institute of Respiratory Disease, First Affitiated Hospital of Guangzhou Medical College, Guangzhou 510120, Guangdong, China; 3.ME Key Laboratory of Zoonosis, Institute of Zoonosis, Jilin University, Changchun 130062, Jilin, China
关键词:
人骨髓间充质干细胞; 心肌损伤; 细胞移植; 归巢; 分化
Keywords:
human bone marrow mesenchymal stem cells;  myocardial damage;  cellular transplant;  homing;  differentiation
分类号:
R617
DOI:
-
文献标识码:
A
摘要:
目的: 探讨在心肌细胞移植中机体内环境因素对人骨髓间充质干细胞（MSC）向心肌细胞（CM）定向分化的影响。方法: 取人骨髓血，用Percoll（1 073 g/L）进行密度梯度离心及贴壁筛选结合的方法，体外培养扩增人骨髓MSC，以流式细胞仪鉴定其纯度，并进行免疫细胞化学染色。随后将人骨髓MSC植入异丙肾上腺素所致急性心肌损伤的裸鼠体内。3周后，取心脏组织块进行免疫组织化学染色检测。 结果: 体外分离纯化培养扩增的人骨髓MSC，可高表达CD44，CD105和波形蛋白，不表达CD31，CD34，CD45，肌钙蛋白I和结蛋白。细胞移植3周后，人骨髓MSC在心肌损伤区域均能分化为表达肌钙蛋白I，结蛋白和波形蛋白的细胞。 结论: 体外分离培养扩增的人骨髓MSC可归巢于受损心脏，并参与体内心肌损伤的修复，具有向CM分化的潜能。
Abstract:
AIM: To explore the effect of organic internal environment on directional differentiation of human bone marrow mesenchymal stem cells (hBM-MSCs) during transplantation of cardiac muscle cells. METHODS: BM-MSCs were identified by flow cytometry and immunocytochemical staining. hMSCs were then injected by vena caudalis into athymic mouse with myocardial damage done by isoprenaline as cardiac cellular transplant. Three weeks after cellular transplant, the damaged heart was immunohistochemically stained. RESULTS: hBM-MSCs highly expressed CD44, CD105 and vimentin but not CD31, CD34, CD45, troponin I and desmin. The cells differentiated from hBM-MSCs in the heart of athymic mouse with myocardial infarction positively expressed troponin I, desmin and vimentin. CONCLUSION: hBM-MSCs can be cultured and proliferated in vitro. hBM-MSCs are signaled and recruited in myocardial damage where they undergo differentiation into cardiomyocytes and may participate in the healing of myocardial damage.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2008-9-19.基金项目：国家高技术研究发展计划863计划项目资助(2004AA205020)；吉林省政府重大专项项目资助(30370548)；吉林大学青年教师科学基金项目资助(419070100049) 通讯作者：李玉林，教授，主要从事肿瘤间质血管病理生物学及干细胞组织工程学的研究Email:ylli@mail.jlu.edu.cn 作者简介：邓方阁，主治医师，博士Email：parisdeng256.student@sina.com
更新日期/Last Update: 2009-09-30