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|本期目录/Table of Contents|

转化生长因子β1/Smad通路调控糜酶诱导的大鼠心脏成纤维细胞增殖

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2010年第1期
页码:
25-29
栏目:
基础研究
出版日期:
2010-01-04

文章信息/Info

Title:
Effect of transforming growth factor-β1/Smad pathway on proliferation of chymase-induced cardiac fibroblasts
作者:
赵晓燕1; 2; 赵连友1; 郑强荪1
1.第四军医大学唐都医院心内科，陕西 西安 710038；2.解放军第五医院心肾内科，宁夏 银川 750004
Author(s):
ZHAO Xiao-yan1; 2;  ZHAO Lian-you1;  ZHENG Qiang-sun1
1.Department of Cardiology, Tangdu Hospital, Fourth Military Medical University, Xi’an 710038, China; 2.Department of Cardiology, Fifth Hospital of PLA, Yinchuan 750004, Ningxia, China
关键词:
糜酶; 心脏成纤维细胞; 增殖; 转化生长因子β1; Smad
Keywords:
chymase;  cardiac fibroblast;  proliferation;  transforming growth factor-β1;  Smad　
分类号:
R392.12
DOI:
-
文献标识码:
A
摘要:
目的: 探讨转化生长因子β1(TGF-β1)/Smad (线虫Sma分子和果蝇Mad分子的组合)信号通路对糜酶诱导大鼠心脏成纤维细胞（CFs）增殖的影响。方法: 用胰酶消化法分离、培养新生SD大鼠的CFs。采用氚标记胸腺嘧啶核苷(3H-TdR)掺入法测定CFs的DNA合成，Western blot检测TGF-β1、Smad2/3、p-Smad2/3及Smad7的蛋白的表达。结果: ①糜酶可以浓度依赖的方式增加CFs的3H-TdR掺入率。15、30和60 μg/L组3H-TdR的掺入率分别为(319±29)、(372±43)和(401±47)cpm/孔，均高于对照组［(252±35)cpm/孔，P<0.01］。②随着糜酶作用浓度的增加，CFs中TGF-β1蛋白的表达呈递增趋势。15、30和60 μg/L组的TGF-β1蛋白的表达水平分别为0.968±0.069、1.782±0.058和2.656±0.085，均较对照组(0.333±0.023)显著升高(P<0.05或P<0.01）。③糜酶可以浓度依赖的方式上调p-Smad2/3蛋白的表达、下调Smad7蛋白的表达，15、30和60 μg/L组的p-Smad2/3蛋白表达的水平均较对照组显著升高（P<0.01），Smad7蛋白表达的水平均较对照组明显降低（P<0.05或P<0.01）。不同浓度的糜酶对Smad2/3蛋白的表达均无明显影响。结论: 糜酶具有促进CFs 增殖的作用，其机制可能与TGF-β1/Smad信号通路的活化有关。
Abstract:
AIM: To explore the effect of growth factor-β1 (TGF-β1)/Smad pathway on the proliferation of chymase-induced rat cardiac fibroblasts (CFs). METHODS: CFs from neonatal Sprague Dawley rats isolated by trypsinization were cultured. DNA synthesis of the CFs was determined by ［3H］-TdR incorporation and protein expressions of TGF-β1, p-Smad2/3, Smad2/3 and Smad7 in CFs were determined by Western blot. RESULTS: Chymase increased the ［3H］-TdR incorporation of CFs in a concentration-dependent manner. The ［3H］-TdR incorporations induced by 15, 30 and 60 μg/L chymase were (319±29), (372±43) and (401±47) cpm/well, respectively, significantly higher than in control groups (252±35) cpm/well, P<0.01). Chymase also increased the expression of TGF-β1 protein in a concentration-dependent manner. TGF-β1 protein expressions in 15, 30 and 60 μg/L chymase groups were 0.968±0.069, 1.782±0.058 and 2.656±0.085, respectively, significantly higher than in control groups (0.333±0.023, P<0.05 or P<0.01). Also, 15, 30 and 60 μg/L chymase obviously increased the p-Smad2/3 expression and decreased Smad7 protein expression in a concentration-dependent manner as compared with control groups (P<0.05 or P<0.01) but had no effect on the protein expression of Smad2/3. CONCLUSION: Chymase promotes proliferation of rat CFs and the increase may be related to the activation of TGF-β1/Smad signaling pathway.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2009-7-6.基金项目：陕西省科学技术研究发展计划项目资助（2005K13-G1-3） 作者简介：赵晓燕，副主任医师，博士Email:zxysxy420@yahoo.com.cn
更新日期/Last Update: 2010-01-05