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|本期目录/Table of Contents|

内皮祖细胞移植对兔心肌梗死后心功能的影响

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2010年第1期
页码:
56-58
栏目:
基础研究
出版日期:
2010-01-04

文章信息/Info

Title:
Effect of endothelial progenitor cell transplantation on cardiac function in rabbits with myocardial infarction
作者:
许爱斌1; 宋延彬2; 郭文怡1; 李飞1; 王海昌1; 谢晓莉1
1.第四军医大学西京医院心内科，陕西 西安 710032；2.延安大学附属医院心内科,陕西 延安 716000
Author(s):
XU Ai-bin1;  SONG Yan-bin2;  GUO Wen-yi1;  LI Fei1;  WANG Hai-chang1;  XIE Xiao-li1
1.Department of Cardiology, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, Shaanxi, China; 2.Department of Cardiology, Affiliated Hospital of Yan'an University, Yan’an 716000, Shaanxi, China
关键词:
内皮祖细胞; 心肌梗死; 心功能; 兔
Keywords:
endothelial progenitor cells;  myocardial infarction;  cardiac function;  rabbits
分类号:
R542. 2
DOI:
-
文献标识码:
A
摘要:
目的: 探讨移植内皮祖细胞(EPCs)对兔心肌梗死后心功能的影响。 方法: 通过密度梯度离心法体外分离兔单个核细胞，接种于人纤连蛋白包被的培养板上，并给予含血管内皮生长因子(VEGF）20 μg/L的EBM-2完全培养液培养2周。取健康大耳白兔，结扎左冠状动脉前降支，并将制作心肌梗死模型成功的大耳白兔随机分为两组，即对照组和实验组，每组20只。2周后实验组心外膜下直接注射EPCs（1.6×1010/L）悬液，对照组注射无菌PBS液。此外，两组均给予心肌梗死后常规药物治疗。术后4周，分别行超声心动图检查和血浆脑钠肽(BNP)浓度测定及计数移植区毛细血管。 结果: 实验组左室舒张末期内径(LVEDD)与术前及对照组相比均无统计学差异，而实验组左室射血分数(LVEF)与术前及对照组相比有统计学差异(P<0.05)。实验组血浆BNP水平较术前降低，差异有统计学意义(P<0.01)，且与对照组相比，血浆BNP水平明显降低(P<0.05)。移植细胞4周后，实验组梗死边缘区毛细血管密度与对照组有显著性差异(P<0.05)。结论: 心外膜下直接注射移植EPCs能够改善兔心肌梗死后的心功能，延缓慢性心力衰竭的发生和发展。
Abstract:
AIM: To explore the effect of endothelial progenitor cell transplantation on cardiac function in rabbits with myocardial infarction. METHODS: Forty rabbits were randomly divided into two groups: experiment group and control group. After two weeks, myocardial infarction was induced by ligating the left anterior descending branch of the coronary artery and both groups received standard drug therapy. Four weeks after transplantation, changes in cardiac function were examined by echocardiography and brain natriuretic peptide (BNP) levels. RESULTS: Left ventricular ejection fraction (LVEF) and sizes of LVEDD in post-transplantation group were not significantly different from those pre-transplantation and in control group. Plasma BNP level decreased significantly at 4 weeks after endothelial progenitor cell transplantation compared with pre-transplantation and in control group (P<0.05). CONCLUSION: Endothelial progenitor cell transplantation improves cardiac function after myocardial infarction.

参考文献/References

［1］ Urbich C, Dimmeler S. Endothelial progenitor cells: characterizationand role in vascular biology［J］. Circ Res, 2004, 95(4):343-353.

［2］ Zhou B, Cao XC, Fang ZH, et al. Prevention of diabetic microangiopathy by prophylactic transplant of mobilized peripheral blood mononuclear cells［J］. Acta Pharmacol Sin, 2007, 28(1):89-97.

［3］ Zhou B, Ma FX, Liu PX, et al. Impaired therapeutic vasculogenesis by transplantation of OxLDL-treated endothelial progenitor cells［J］. J Lipid Res, 2007, 48(3):518-527.

［4］ Yang C, Zhang ZH, Li ZJ, et al. Enhancement of neovascularization with cord blood CD133+ cell-derived endothelial progenitor cell transplantation［J］. Thromb Haemost, 2004, 91(6):1202-1212.

［5］ Li S, Zhou B, Han ZC. Therapeutic neovascularization by transplantation of mobilized peripheral blood mononuclear cells for limb ischemia: a comparison between CD34+ and CD34- mononuclear cells［J］. Thromb Haemost, 2006, 95(2):301-311.

备注/Memo

备注/Memo:
收稿日期：2009-4-2.通讯作者：郭文怡，主任医师，主要从事冠心病介入治疗研究Email:guowenyi@tom.com 作者简介：许爱斌：医师，硕士生Email:xuaibingeneral@sina.com
更新日期/Last Update: 2010-01-05