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|本期目录/Table of Contents|

丙泊酚对老年患者外周血淋巴细胞上β1肾上腺素能受体脱敏的影响

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2010年第1期
页码:
107-110
栏目:
临床研究
出版日期:
2010-01-04

文章信息/Info

Title:
Role of propofol in peripheral lymphocytic β1 adrenergic receptor desensitization in elderly patients
作者:
吴坛光; 吴晓智; 陈国忠; 刘韧; 林剑清
南京军区福州总医院麻醉科，福建 福州 350025
Author(s):
WU Tan-guang;  WU Xiao-zhi;  CHEN Guo-zhong;  LIU Ren;  LIN Jian-qing
Department of Anesthesiology, Fuzhou General Hospital, Nanjing Military Area Command, Fuzhou 350025, Fujian, China
关键词:
丙泊酚; 淋巴细胞; β1肾上腺素能受体; 脱敏
Keywords:
propofol;  peripheral lymphocytes;  β1 adrenergic receptor;  desensitization
分类号:
R614.2
DOI:
-
文献标识码:
A
摘要:
目的: 观察丙泊酚单次静脉给药对老年患者外周血淋巴细胞β1肾上腺素能受体（β1-AR）脱敏的影响，并探讨其循环抑制机制。方法: 选择拟在全麻下施行择期手术治疗的老年患者30例，随机分为3组（n=10），即低剂量组(L组)：给予丙泊酚1.0 mg/kg，高剂量组(H组)：给予丙泊酚2.0 mg/kg和对照组(C组)：给予生理盐水。均在全麻诱导前，静脉单次注射给药。给药前后3 min,记录收缩压（SBP）、舒张压（DBP）和心率（HR）的变化，同时抽取外周静脉抗凝血各15 ml，经Boyum法分离淋巴细胞后，用Western blot分别检测总蛋白、胞膜和胞质蛋白中β1-AR的表达;用放射免疫法检测异丙肾上腺素刺激的淋巴细胞内cAMP的含量。结果: 与C组比较，L组和H组给药后均可降低SBP，DBP，淋巴细胞内cAMP含量及胞膜β1-AR蛋白的水平（P<0.05或P<0.01）;但胞质β1-AR蛋白的水平增加（P<0.05或P<0.01）。H组的上述指标降低或增加的幅度均较L组明显(P<0.05)。组间两两比较，淋巴细胞总蛋白中β1-AR的水平无显著差别。结论: 丙泊酚可引起老年患者剂量依赖外周血淋巴细胞上β1-AR脱敏，与丙泊酚可促进β1-AR由胞膜内化进入胞质有关。
Abstract:
AIM: To evaluate the role of propofol in peripheral lymphocytic β1 adrenergic receptor (AR) desensitization in elderly patients. METHODS: Thirty elderly patients were recruited into the study and were classified into three groups (n=10): low-dose group (L) with propofol (1.0 mg/kg), high-dose group (H) with propofol (2.0 mg/kg) and control group (C). Lymphocytes were isolated and expressions of β1-AR in cytoplasm and cytomembrane were detected with Western blot. Isoproterenol-stimulated cAMP products in lymphocytes were measured with radioimmunoassay. Before and after injection, blood pressure (BP) and heart rate (HR) were also recorded. RESULTS: Systolic blood pressure (SBP), diastolic blood pressure (DBP), cAMP product and β1-AR level in lymphocytic cytomembrane in the L group and H group were all lower than in the control group (P<0.05 or P<0.01). On the contrary, β1-AR protein level in lymphocytic cytomembrane in the L group and H group was significantly more abundant than in the control group (P<0.05 or P<0.01). CONCLUSION: Clinically relevant concentrations of propofol attenuate -adrenergic signal transduction in peripheral lymphocytes via promotion to the internalization of β1-AR and inhibition of cAMP production, which may be a contributory factor in cardiac vascular inhibition.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2008-5-15.基金项目：南京军区福州总医院院内课题基金项目资助（200731) 通讯作者：陈国忠，教授，主要从事围术期心肌保护研究Email:Cgzssq2000@sina.com 作者简介：吴坛光，主治医师，硕士Email:wutanguang@126.com 现工作单位：武汉解放军第161中心医院麻醉科
更新日期/Last Update: 2010-01-05