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马钱子碱对大鼠心室肌细胞瞬时外向钾电流的影响

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2010年第2期
页码:
172-175
栏目:
基础研究
出版日期:
2010-03-04

文章信息/Info

Title:
Effects of brucine on transient outward potassium current of ventricular myocytes of rats
作者:
甘文云; 唐其柱; 王腾; 王蕾; 田青; 周恒; 郭海鹏
武汉大学人民医院心内科，湖北 武汉 430060
Author(s):
GAN Wen-yuen;  TANG Qi-zhu;  WANG Teng;  WANG Lei;  TIAN Qing;  ZHOU Heng;  GUO Hai-peng
Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei, China
关键词:
马钱子碱; 瞬时外向钾电流; 全细胞膜片钳技术; 心室肌细胞; 大鼠
Keywords:
brucine;  transient outward potassium current;  whole-cell patch clamp technique;  ventricular cardiomyocytes;  rats
分类号:
R514.7
DOI:
-
文献标识码:
A
摘要:
目的: 探讨马钱子碱(brucine)对大鼠心室肌细胞瞬时外向钾电流(transient outward potassium current，Ito)及其动力学的影响。方法: 用酶解法分离大鼠单个心室肌细胞。用全细胞膜片钳技术记录不同浓度的马钱子碱可对大鼠心室肌细胞Ito的前后影响变化。结果: ①马钱子碱可浓度依赖性地阻断Ito；②5 μmol/L的马钱子碱可使Ito稳态激活曲线右移, V1/2由对照的（-30.7±10.2）mV变为(-27.8±5.8)mV(P<0.05)；③5 μmol/L的马钱子碱可使Ito稳态失活曲线左移，V1/2分别为(-41.6±1.0)mV变为(-75.0±2.8)mV(P<0.05)；④5 μmol/L的马钱子碱可使Ito稳态激活曲线右移失活后再恢复时间常数τ延长。结论: 提示马钱子碱可以阻断Ito，对Ito的激活态和失活态均具有较高的亲和力，这些可能是其抗心律失常作用的机制。
Abstract:
AIM: To explore the effects of brucine on transient outward potassium current (Ito) of ventricular cardiomyocytes of rats. METHODS: Single ventricular myocytes of rats were obtained by enzymatic dissociation. The whole-cell patch-clamp recording technique was used to record the changes of Ito by different dosages of brucine. RESULTS: Brucine significantly reduced Ito in a concentration-dependent manner. Brucine at 5 μmol/L shifted the stabilized activation curve to the right and the half-point of actiation potential (V1/2) from ( 30.7±10.2) mV to ( 27.8±5.8) mV (n=8, P<0.05). Brucine (5 μmol/L) shifted the stabilized deactivation curve to the left and the half-point of actiation potential (V1/2) from (41.6±1.0) mV to (75.0±2.8) mV (n=8, P<0.05). Brucine (5 μmol/L) significantly prolonged the recovery time. CONCLUSION: Brucine has significant inhibitive effects on Ito, which may be one of the mechanisms that underlie its antiarrhythmic effect.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2009-3-3.通讯作者：唐其柱， 教授，主要从事心肌病和心脏电生理的研究Email：qizhut@yahoo.com.cn 作者简介：甘文云，硕士Email:ganwenyun21@sina.com
更新日期/Last Update: 2010-03-05