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《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:

2010年第3期

页码:

347-350

栏目:

基础研究

出版日期:

2010-04-06

文章信息/Info

Title:

Expression of nerve growth factor and sympathetic neural remodeling after myocardial infarction in type II diabetic rats

作者:

刘野¹; 2; 路军¹; 刘雄涛¹; 丁璐¹; 滕继伟¹; 郑强荪¹

1.第四军医大学唐都医院心内科,陕西 西安 710038；2.解放军第208医院心内科，吉林 长春 130062

Author(s):

LIU Ye¹; ²;  LU Jun¹;  LIU Xiong-tao¹;  DING Lu¹;  TENG Ji-wei¹;  ZHENG Qiang-sun¹

1.Department of Cardiology, Tangdu Hospital, Fourth Military Medical University, Xi’an 710038, Shaanxi, China; 2.Department of Cardiology, PLA 208 Hospital, Changchu 130062, Jilin, China

关键词:

糖尿病; 心肌梗死; 交感神经; 神经生长因子; 大鼠

Keywords:

diabetes mellitus;  myocardial infarction;  sympathetic nerve;  nerve growth factor;  rats

分类号:

R587.1

DOI:

-

文献标识码:

A

摘要:

目的: 探讨糖尿病并发心肌梗死(MI)对大鼠心肌中神经生长因子(NGF)的表达及交感神经再生重构的影响。方法: 将实验大鼠分为正常对照组、糖尿病对照组、MI对照组及糖尿病MI组，从各组大鼠的左心室梗死周边、室间隔和右心室取材，通过免疫组化染色并结合计算机图像处理技术，对心肌中NGF蛋白表达及交感神经支配进行对比分析。结果: 与正常对照组及糖尿病心梗组比较，MI对照组的NGF表达均增加（均P<0.05），交感神经支配密度也均明显增加(均P<0.01)，并且二者在梗死周边、室间隔及右心室3个部位的表达存在相关性(均P<0.05)，糖尿病对照组的NGF表达均降低(P<0.05，P<0.01)，交感神经支配密度分别为无明显差异及明显降低(P<0.01)。结论: 糖尿病可抑制心肌细胞表达NGF，但却可促进MI后交感神经的过度再生与重构。推测糖尿病条件下，NGF并非交感神经再生重构的决定因素，尚存在其他途径对交感神经再生及重构进行调节。

Abstract:

AIM: To explore the alteration of the expression of nerve growth factor (NGF) and sympathetic neural remodeling after myocardial infarction in type II diabetic rats. METHODS: Samples were obtained from the peri-infarcted area, ventricular septum and right ventricle (RV) in infarcted diabetic rats. Using immunohistochemistry and computerized image-processing technique, expressions of NGF protein and sympathetic innervation were examined in different groups (blank, diabetic, infarcted, and infarcted diabetic, respectively). RESULTS: In the diabetic group, expressions of NGF protein were lower than those in the blank group (P<0.05) and those in the infarcted diabetic group (P<0.01). The density of sympathetic nerves was also lower than in the infarcted diabetic group (P<0.01). In the infarcted group, expressions of NGF protein and density of sympathetic nerves were both higher than in the blank group (P<0.05, P<0.01) and those in the infarcted diabetic group (P<0.05, P<0.01). NGF protein expression was correlated with sympathetic innervation in the peri-infarcted area, RV and septum in infarcted group (P<0.05). CONCLUSION: Diabetes mellitus decreases the expression of NGF protein in cardiomyocytes but increases sympathetic reinnervation and hyperinnervation, indicating that it may not be NGF but some other factor that plays a crucial role in post-infarct neural remodeling.

参考文献/References

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备注/Memo

备注/Memo:

收稿日期：2009-05-04.通讯作者:郑强荪，主任医师，主要从事心律失常的临床和基础研究Email:qingshu@fmmu.edu.cn 作者简介:刘野，住院医师，硕士生Email:shengxinye9171@sina.com

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更新日期/Last Update: 2010-04-09