我们的网站为什么显示成这样？

可能因为您的浏览器不支持样式，您可以更新您的浏览器到最新版本，以获取对此功能的支持，访问下面的网站，获取关于浏览器的信息：

|本期目录/Table of Contents|

大鼠急性心肌缺血/再灌注模型改良方法与传统方法的比较

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2010年第4期
页码:
533-536
栏目:
基础研究
出版日期:
2010-06-10

文章信息/Info

Title:
Comparison between conventional and modified methods of acute myocardial ischemia/reperfusion models
作者:
刘海涛1; 李飞1; 王跃民2; 陶凌1; 李聪叶1; 苑媛1; 王海昌1
第四军医大学:1.西京医院心血管内科，2.基础部生理教研室，陕西 西安 710032
Author(s):
LIU Hai-Tao1;  LI Fei1;  WANG Yue-min2;  TAO Ling1;  LI Cong-ye1;  YUAN Yuan1;  WANG Hai-chang1
1.Department of Cardiology, Xijing Hospital, 2.Department of Physiology, Fourth Military Medical University, Xi’an 710032, Shaanxi, China
关键词:
心肌缺血/再灌注; 模型; 改良方法; 传统方法; 大鼠
Keywords:
rat;  acute myocardial ischemia/reperfusion;  modified model
分类号:
R542.2
DOI:
-
文献标识码:
A
摘要:
目的: 建立大鼠急性心肌缺血/再灌注模型的改良方法与传统方法并进行比较。方法: 将60只SD大鼠随机分为两组，每组30只；一组采用改良方法建立模型，即气体麻醉，以气体麻醉面罩通气，短时间开胸；另一组采用传统方法建立模型，即腹腔麻醉，气管插管，长时间开胸。比较两种方法的手术时间、手术成功率、术中血氧饱和度、术后存活率、心肌梗死面积，以及术后大鼠的生活状况。结果: 与传统方法组相比，改良方法组大鼠术中存活率升高［(93.3±4.6)% vs.(72.4±8.4)%,P<0.05］、开胸时间缩短为［(6.5±2.0) min vs.(44.9±2.7) min,P<0.01］，开胸前后平均血氧饱和度增加［(96.3±0.8)% vs.(90.9±2.1)%,P<0.05］，术后存活率提高(82.83% vs. 58.3%,P<0.01)。另外改良方法组大鼠术后恢复正常行为活动需要的时间较短，但两组大鼠心肌梗死的面积［(33.5±2.2)% vs. (35.0±3.0)%］无统计学意义。结论: 改良方法具有简单、高效及可独立操作的优点，能有效提高建立大鼠急性心肌缺血/再灌注模型的成功率。
Abstract:
AIM: To develop and compare the efficacy of a modified vs. conventional rat model of acute myocardial ischemia/reperfusion. METHODS: Sixty Sprague Dawley (SD) rats were randomly divided into two groups: a modified group (respiratory mask support and short-time chest opening) and a conventional group (tracheal intubation and long-time chest opening). Surgical time, surgical success rate, survival rate, and infarct size were investigated, and the postoperative survival rate of the rats was observed. RESULTS: During the perioperative period, the surgical success rate was higher in the modified model ［(93.3±4.6)% vs. (72.4±8.4)%, P<0.05］. Chest-opening time was shorter in the modified group vs. the conventional model ［(6.5±2.0) min vs. (44.9±2.7) min, P<0.01］. Postoperative resumption of normal behaviors and activities was quicker in the modified group, with a statistically significant higher survival rate compared with that in the conventional group (82.83% vs. 58.3%, P<0.001). Triphenyltetrazolium chloride staining showed no significant difference in infarct size between groups ［(33.5±2.2)% vs. (35.0±3.0)%］. CONCLUSION: The modified method offers the advantages of simplicity, efficiency and independent operation, which enhances the success rate of establishing the acute rat myocardial ischemia/reperfusion model.

参考文献/References

［1］Kloner RA, Braunwald E. Observations on experimental myocardial ischaemia［J］. Cardiovasc Res, 1980, 14(7):371-395.

［2］Verdouw PD, van den Doel MA, de Zeeuw S, et al. Animal models in the study of myocardial ischaemia and ischaemic syndromes［J］. Cardiovasc Res, 1998, 39(1):121-135.

［3］Grund F, Sommerschild HT, Kirkeboen KA, et al. A new approach to normalize myocardial temperature in the open-chest pig model［J］. J Appl Physiol, 1998, 84(6):2190-2197.

［4］ Duncker DJ, Klassen CL, Ishibashi Y, et al. Effect of temperature on myocardial infarction in swine［J］. Am J Physiol, 1996, 270(4 Pt 2):H1189- H1199.

［5］Schwartz LM, Verbinski SG, Vander HRS, et al. Epicardial temperature is a major predictor of myocardial infarct size in dogs［J］. J Mol Cell Cardiol, 1997, 29(6):1577-1583.

[6]Hale SL, Dave RH, Kloner RA. Regional hypothermia reduces myocardial necrosis even when instituted after the onset of ischemia［J］. Basic Res Cardiol, 1997, 92(5):351-357.

[7]肖骏,佘强,罗开良,等. 大鼠慢性心肌梗死模型制备的改进［J］. 四川动物, 2008, 27(3):396-398.

[8]Liang F, Gao E, Tao L, et al. Critical timing of L-arginine treatment in post-ischemic myocardial apoptosis-role of NOS isoforms［J］. Cardiovasc Res, 2004, 62(3):568-677.

[9] Liu HT, Zhang HF, Si R, et al. Insulin protects isolated hearts from ischemia/reperfusion injury: cross-talk between PI3-K/Akt and JNKs［J］. Sheng Li Xue Bao, 2007, 59(5):651-659.

[10]Li J, Zhang H, Wu F, et al. Insulin inhibits tumor necrosis factor-alpha induction in myocardial ischemia/reperfusion: role of Akt and endothelial nitric oxide synthase phosphorylation［J］. Crit Care Med, 2008, 36(5):1551-1558.

[11]Su H, Sun X, Ma H, et al. Acute hyperglycemia exacerbates myocardial ischemia/reperfusion injury and blunts cardioprotective effect of GIK［J］. Am J Physiol Endocrinol Metab, 2007, 293(3):E629-E635.

[12]朱彤莹,黄国英,顾勇,等. 两种心衰模型大鼠心功能的比较［J］. 中国实验动物学报, 2002, 10(1):51-53.

[13]Zhao X, Wu N, Zhou J, et al. A technique for retrograde intubation in mice［J］. Lab Anim (NY), 2006, 35(3):39-42.

[14]Yu QJ, Si R, Zhou N, et al. Insulin inhibits beta-adrenergic action in ischemic/reperfused heart: a novel mechanism of insulin in cardioprotection［J］. Apoptosis, 2008, 13(2):305-317.

［15］Toyota E, Kawaguchi Y, Ogasawara Y, et al. Novel rat model of ischemic cardiomyopathy induced by repetitive myocardial ischemia/reperfusion injury while conscious［J］. Circ J, 2007, 71(5):788-795.

备注/Memo

备注/Memo:
收稿日期：2009-07-27.基金项目：国家自然科学基金项目课题(30770784) 通讯作者：王海昌，主任医师，主要从事冠心病与心律失常的诊治研究Email:wanghc@fmmu.edu.cn 作者简介：刘海涛，博士生Email:xjcardio@hotmail.com
更新日期/Last Update: 2010-05-20