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|本期目录/Table of Contents|

氧化型低密度脂蛋白对可诱导共刺激分子在人外周血T细胞表达的影响

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2010年第6期
页码:
807-810
栏目:
基础研究
出版日期:
2010-08-23

文章信息/Info

Title:
Effects of oxidized low-density lipoprotein on expression of inducible costimulator in human peripheral blood T cells
作者:
徐琳1*; 朱肖星2*; 张亚莉3*; 彭智欣1; 李炳玲1; 李志梁4; 邱健1
1.广州军区广州总医院心血管内科，广东 广州 510010；2.第四军医大学干休所，陕西 西安 710032； 3.贵阳医学院医学检验系，贵州 贵阳 550004；4.南方医科大学珠江医院心血管内科，广东 广州 510280
Author(s):
XU Lin;  ZHU Xiao-xing;  ZHANG Ya-li;  PENG Zhi-xin;  LI Bing-ling;  LI Zhi-liang;  QIU Jian
Department of Cardiology, PLA Guangzhou General Hospital, Guangzhou Military Area Command, Guangzhou 510010, Guangdong, China
关键词:
可诱导共刺激分子; 氧化低密度脂蛋白; 人外周血T细胞; 动脉粥样硬化
Keywords:
inducible costimulator;  oxidized low-density lipoprotein;  human peripheral blood T cells;  atherosclerosis;  immune mechanism
分类号:
R392.12
DOI:
-
文献标识码:
A
摘要:
目的: 探讨可诱导共刺激分子（ICOS）在人外周血T细胞的表达及氧化低密度脂蛋白(ox-LDL)的干预作用。方法: 以人外周血T细胞为实验模型，通过间接免疫荧光法、RT-PCR和Western blot等方法，观察ox-LDL对人外周血T细胞表达ICOS的影响。结果: ①ICOS表达于人外周血T细胞膜，荧光信号呈点状散在分布于细胞表面。RT-PCR检测显示，ICOS mRNA的扩增片段位于相当于Marker 368 bp的位置。Western blot检测显示，ICOS的分子量约为55 kD。②ox-LDL刺激组ICOS的吸光度(A)值高于空白对照组，提示ox-LDL能够明显增加人外周血T细胞中的ICOS mRNA和其蛋白的表达（P<0.05）。结论: ICOS表达于人外周血T细胞表面，ox-LDL能够上调ICOS mRNA和其蛋白的表达，这可能是动脉粥样硬化的免疫学发病机制之一。
Abstract:
AIM: To study the expression of inducible costimulator (ICOS) on human peripheral blood T cells (HPBTC) and the intervening effect of oxidized low-density lipoprotein (ox-LDL). METHODS: HPBTCs were cultured in vitro and incubated with 100 mg/L ox-LDL for 24 h. ICOS expression levels were determined by fluorescence, reverse transcription PCR (RT-PCR) and Western blotting, respectively. RESULTS: Fluorescence microscopy showed that fluorescence signals of positive cells expressed by ICOS were distributed on cell membrane in a spot-style. RT-PCR of mRNA derived from the cultured HPBTC showed products of the expected size (368 bp). Western blotting of protein verified HPBTC expression of ICOS as a cell-associated protein with a molecular mass of 55 kDa. ox-LDL significantly increased in the expression of ICOS. Similar effects were seen in both expressions of mRNA and protein (P<0.05). CONCLUSION: ICOS can express on HPBTC and ox-LDL upregulates the expression of ICOS, which may be one of the immunomechanisms of atherosclerosis.

参考文献/References

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［2］Haimila KE, Partanen JA, Holopainen PM. Genetic polymorphismof the human ICOS gene［J］. Immunogenetics, 2002, 53(12):1028-1032.

［3］Vink H, Constantinescu AA, Spaan JA. Oxidized lipoproteins degrade the endothelial surface layer :implications for platelet-endothelial cell adhesion［J］. Circulation, 2000, 101(13):1500-1502.

［4］Asgary S, Saberi SA, Azampanah S. Effect of immunization against ox-LDL with two different antigens on formation and development of atherosclerosis［J］. Lipids Health Dis, 2007, 6:32-36.

［5］徐琳,李志梁,洪长江,等. B7相关蛋白-1在人冠状动脉内皮细胞的表达及临床意义［J］. 南方医科大学学报, 2009, 29(8):1623-1625.

［6］徐琳，李志梁，朱肖星，等. 氧化低密度脂蛋白刺激人冠状动脉内皮细胞可诱导共刺激分子配体的表达［J］. 细胞与分子免疫学杂志, 2009， 25(9):819-821.

［7］Watanabe M, TakagiY, Kotani M, et al. Down-Regulation of ICOS Ligand by Interaction with ICOS Functions as a Regulatory Mechanism for Immune Responses［J］. J Immunol, 2008, 180(8):5222-5234.

［8］Gotsman I, Grabie N, DaCosta R, et al. The Role of the T-cell Inducible Co-stimulatory Molecule ICOS in the Development of Atherosclerosis［J］. FASEB J, 2006, 20(3):A199-A205.

[9]Asgary S, Saberi SA, Azampanah S. Effect of immunization against ox-LDL with two different antigens on formation and development of atherosclerosis［J］. Lipids Health Dis, 2007, 6(24):32-38.

[10]严金川,吴宗贵,陈金明,等. 氧化低密度脂蛋白及抗氧化剂对人脐静脉内皮细胞表达CD40和CD40L的影响［J］. 中国微循环, 2002, 6(1):15-17.

[11]毛庆,李浪,梁秀琳. 急性冠脉综合征CD40-CD40L昼夜节律的变化［J］. 心脏杂志, 2008, 20(6):725-727.

［12］ Lutgens E, Lievens D, Beckers L, et al. CD40 and its ligand in atherosclerosis［J］. Trends Cardiovasc Med, 2007, 17(4):118-123.

备注/Memo

备注/Memo:
收稿日期：2009-09-04.基金项目：中国博士后科学基金资助（20080440215）；广东省自然科学基金资助（2157000068,8151051501000062）；广东省和 广州市科技计划项目（2009B11000018，2009JI-c491） 通讯作者：邱健，教授，主任医师，主要从事冠心病介入治疗Email:fly198013@yahoo.com.cn 共同通讯作者：李志梁，教授，主任医师，主要从事冠心病发病机制的研究Email:linx-1111@163.com 作者简介：徐琳，主治医师，博士Email:lynne1111@126.com *并列第一作者
更新日期/Last Update: 2010-08-22