我们的网站为什么显示成这样？

可能因为您的浏览器不支持样式，您可以更新您的浏览器到最新版本，以获取对此功能的支持，访问下面的网站，获取关于浏览器的信息：

|本期目录/Table of Contents|

左旋卡尼汀在犬心房急性电重构中的抗氧化作用

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2011年第1期
页码:
7-10
栏目:
基础研究
出版日期:
2010-10-27

文章信息/Info

Title:
Anti-oxidative stress effects of L-carnitine on acute atrial electrical remodeling in dogs
作者:
官媛1; 2; 单兆亮1; 郑强荪2; 张晔1; 郭红阳1; 王玉堂1
1.解放军总医院心内科，北京 100843；2.第四军医大学唐都医院心内科，陕西 西安 710038
Author(s):
GUAN Yuan1; 2;  SHAN Zhao-liang1;  ZHENG Qiang-sun2;  ZHANG Ye1;  GUO Hong-yang1;  WANG Yu-tang1
1.Department of Cardiology, PLA General Hospital, Beijing 100853, China; 2.Department of Cardiology, Tangdu Hospital, Fourth Military Medical University, Xi’an 710038, Shaanxi, China
关键词:
心房颤动; 犬; 氧化应激; 电重构; 左旋卡尼汀
Keywords:
atrial fibrillation;  dogs;  oxidative stress;  electrical remodeling;  L-carnitine
分类号:
R541.7
DOI:
-
文献标识码:
A
摘要:
目的: 探讨左旋卡尼汀(L-carnitine,L-CN)抑制犬心房颤动（房颤）时心房急性电重构的作用机制。方法： 18条成年杂种犬，随机分为生理盐水（NS）组、L-CN组和正常对照 (normal control，NC) 组。NS组予右心房600次/min快速起搏，诱发并维持房颤2 h；起搏前半小时静推生理盐水(300 mg/kg) ，继以液速50 ml/h维持静脉点滴2 h。L-CN组：右心房600次/min快速起搏，诱发并维持房颤2 h，起搏前半小时静推L-CN(300 mg/kg)，继以2.5 mg/（kg·min）（液速50 ml/h）维持静脉点滴2 h。在不同观察时间点测定房颤前后电生理指标及静脉血氧化及抗氧化指标的变化。结果： ①房颤后NS组心房有效不应期（AERP）显著缩短(P<0.01)，AERP的频率适应性显著下降(P<0.01)；L-CN组房颤前后AERP和频率适应性无显著缩短;L-CN组较NS组房颤诱发率明显降低(P<0.05)。②NS组房颤时静脉血氧化应激产物较NC组显著增多(P<0.05)，抗氧化指标显著下降(P<0.01)。③ L-CN组丙二醛（Malondialdehyd,MDA）生成显著减少(P<0.01)，超氧化物歧化酶（superoxide dismutase,SOD）显著增加(P<0.05)。结论： L-CN对房颤时氧化应激及电重构有明显保护作用，这种保护机制可能通过清除自由基，抑制脂质过氧化而发挥作用。
Abstract:
AIM: To evaluate the anti-oxidative stress effects of L-carnitine (L-CN) on tachycardia-induced acute atrial electrical remodeling in dogs. METHODS: Eighteen dogs were randomly divided into three groups: normal saline (NS) group, L-CN group and normal control (NC) group. In both NS and L-CN groups, right atrium was paced at 600 beats per minute (bpm) to induce atrial fibrillation (AF) and maintained for 2 h; 0.9% normal saline (300 mg/kg, IV) or L-carnitine (300 mg/kg, IV) was given before the pace and 0.9% normal saline or L-carnitine ［2.5 mg/(kg·min), 50 ml/h, ID］ was administered for 2 h. The observed atrial electrophysiology indexes included atrial effective refractory period (AERP), index of the AERP rate adaptation (AERP200/AERP150)/50 msec, conduction velocity (CV) and AF induced rate. Oxidation index of malondialdehyde (MDA) and antioxidation index of superoxide dismutase (SOD) of venous blood were measured before and 1 and 2 h after AF was measured. RESULTS: AERP shortened dramatically 2 h after AF (P<0.01) in NS group but no remarkable change was found in L-CN group. AERP rate adaptation in the right atrium decreased significantly (P<0.01) in NS group but did not change obviously in L-CN group. The AF-induced rate in L-CN group was significantly reduced (P<0.01). The oxidation index level (MDA) was increased and anti-oxidation index level (SOD) was decreased in both NS group and L-CN group (P<0.05). L-CN group had a lower MDA level and a higher SOD level compared with those in NS group (P<0.01). CONCLUSION: L-CN prevents tachycardia-induced oxidative stress and acute atrial electrical remodeling in dogs, possibly by cleaning the reactive oxygen species and inhibiting lipid peroxidation.

参考文献/References

[1]Sharifov OF, Zaitser AV, Rosenshtraukh LV, et al. Spatial distribution and frequency dependence of arrhythogenic vagal effects in canine atria[J]. J Cardioasc Ele trophysiol, 2000, 11(9):1029-1042．

[2]刘鹏， 郭继鸿，张海澄，等. 去自主神经条件下迷走神经对肺静脉不同部位房颤诱发率的影响[J]. 中华医学杂志, 2006, 86(5):317-320.

[3]Fujisawa S, Kobayashi A, Hironaka Y, et al. Effects of L- carnitine on the cellular distribution of carnitine and its acy-l derivatives in the ischemic heart[J]. Jpn Heart J, 1992, 33(5):693-705.

[4]蒋雄刚,李平,张凯伦. 左旋卡尼汀对离体心肌缺血再灌注损伤的保护作用[J]. 中华实验外科杂志, 2006, 23(9):1038-1040.

[5]夏经钢，王丽娟，胡健. 左旋卡尼汀对兔在心肌缺血/再灌注损伤状态下抗氧化指标分析[J]. 中国医科大学学报, 2006, 35(4):364-366.

[6] 阮骊韬，曾铁生，段云友，等. 慢性高同型半胱氨酸血症兔氧化应激在血管内皮功能损伤中的作用[J].心脏杂志, 2003, 15(4):311-315.

[7]刘宇宏，曾秋棠，毛晓波，等. 氟伐他汀防治缺血性心脏病心衰及其抗氧化应激作用的实验研究[J]. 心脏杂志, 2005, 17(1)：12-28.

[8]Korantzopoulos P, Kolettis TM, Galaris D, et al. The role of oxidative stress in the pathogenesis and perpetuation of atrial fibrillation[J]. Int J Cardiol, 2007, 115(2):135-143.

[9]Van Wagoner DR. Oxidative stress and inflammation in atrial fibrillation: role in pathogenesis and potential as a therapeutic target[J]. J Cardiovasc Pharmacol, 2008, 52(4):306-313.

[10]Neuman RB, Bloom HL, Shukrullah I, et al. Oxidative stress markers are associated with persistent atrial fibrillation[J]. Clinical Chemistry, 2007, 53(9):1652-1657.

[11]Carnes CA, Chung MK, Nakayama T, et al. Ascorbate attenuates atrial pacing induced peroxynitrite formation and electrical remodeling and decreases the incidence of postoperative atrial fibrillation[J]. Circ Res, 2001, 89(6):322-338.

[12]Dudley SC Jr, Hoch NE, McCann LA, et al. Atrial fibrillation increases production of superoxide by the left atrium and left atrial appendage: role of the NADPH and xanthine oxidases[J]. Circulation, 2005, 112(9):1266-1273.

[13]Kim YH, Lim DS, Lee JH, et al. Gene expression profiling of oxidative stress on atrial fibrillation in humans[J]. Exp Mol Med, 2003, 35(5):623-649.

[14]Lee SH, Lin FY, Yu WC, et al. Regional difference in the recovery course of tachycardia-induced changes of atrial electro-physiological properties[J]. Circulation, 1999, 99(9)：1255-1264．

[15]许纲,吴冬燕,陈元禄,等. 犬急性心房颤动电重构现象的实验研究[J]. 中华心律失常学杂志, 2003, 6(7):157-161.

[16]闫俊瑾，王玉堂，单兆亮，等. 左旋卡尼汀对犬心房急性电重构的影响[J]. 心脏杂志, 2006, 18(3)：284-292.

[17]Elahi MM, Flatman S, Matata BM, et al. Tracing the origins of postoperative atrial fibrillation: the concept of oxidative stress-mediated myocardial injury phenomenon[J]. Eur J Cardiovasc Prev Rehabil, 2008, 15(6):735-741.

备注/Memo

备注/Memo:
收稿日期：2010-02-23.基金项目：国家自然科学基金项目资助(30772886) 通讯作者：单兆亮，副主任医师，主要从事心脏电生理研究Email：shanzl301@sina.com 作者简介：官媛，住院医师，硕士生Email：90902004@sina.com
更新日期/Last Update: 2010-10-27