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波生坦对低氧性肺动脉高压大鼠肺动脉钙调节蛋白表达的影响

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2011年第1期
页码:
31-34
栏目:
基础研究
出版日期:
2010-10-27

文章信息/Info

Title:
Effects of bosentan on expression of calponin in pulmonary arteries in rats with hypoxic pulmonary hypertension
作者:
何璐1; 张玉顺1; 刘海莲2; 朱妙章3
1.西安交通大学第一附属医院心内二科，陕西 西安 710061；2.海军总医院心内科，北京 100037；3.第四军医大学基础部生理学教研室，陕西 西安710032
Author(s):
HE Lu1;  ZHANG Yu-shun1;  LIU Hai-lian2;  ZHU Miao-zhang3
1.Second Department of Cardiology, First Affiliated Hospital, Medical College, Xi’an Jiaotong University, Xi’an 710061, Shaanxi, China; 2.Department of Cardiology, Navy General Hospital, Beijing 100037, China; 3.Department of Physiology, School of Basic Medicine, Fourth Military Medical University, Xi’an, 710032, Shaanxi, China
关键词:
低氧性肺动脉高压; 波生坦; 钙调节蛋白; 大鼠
Keywords:
hypoxic pulmonary hypertension;  bosentan;  calponin;  rats
分类号:
R543.2　
DOI:
-
文献标识码:
A
摘要:
目的: 探讨波生坦（bosentan，BST）对低氧性肺动脉高压（HPH）大鼠肺动脉中钙调节蛋白（calponin，CPN）表达的影响。 方法： 40只健康SD大鼠随机分为正常组、模型组、安慰剂组和BST组，每组各10只。正常组常压常氧下饲养6周，其他组分别置于全自动调节的低压低氧仓中间断低氧(8 h/d)，分别饲养3周、6周和6周。自第4周起，模型组常压常氧下饲养；安慰剂组和BST组大鼠在入仓前分别给予生理盐水（2 ml）和BST（100 mg/kg）灌胃。以6周为观察终点，经HE染色后，观察各组大鼠肺血管的形态学变化；应用图像采集处理系统检测各组大鼠中型及小型肺动脉的相对中膜厚度(RMT)；用免疫组化染色法对各组大鼠肺动脉的CPN进行定位及半定量分析。结果： ①与其他组相比，安慰剂组大鼠肺动脉管壁增厚、管腔狭窄；BST组大鼠肺动脉管壁的厚度及管腔大小可恢复至正常组的状态；②各组大鼠肺动脉管壁中均有CPN表达，随着低氧时间的延长，CPN表达减少，BST组CPN表达与正常组相比无统计学差异。结论： BST可促进HPH大鼠肺动脉CPN的合成。
Abstract:
AIM: To investigate the effect of bosentan on the expression of calponin in pulmonary arteries in rats with hypoxic pulmonary hypertension. METHODS: Forty healthy Sprague Dawley rats were randomly divided into four groups: control group, model group, placebo group and bosentan group, with ten rats in each group. The rats in the control group were kept under a normal environment for 6 weeks and the rats in the other groups were kept in a hypobaric chamber simulating the 5 000 m altitude 8 h/day for, respectively, 3 weeks, 6 weeks and 6 weeks. From the fourth week on, the rats in the model group were kept under a normal environment. For placebo group or bosentan group, sodium chloride (2 ml) or bosentan (100 mg/kg) was given for 3 weeks starting from the fourth week. The sixth week was taken as the end point. H/E stain was used to observe the morphological changes of pulmonary vessels and image analysis system was used to detect the relative medial thickness (RMT) of small- and medium-sized pulmonary arteries. Immunohistochemical technique was used to assess the localization and semiquantitation of calponin in pulmonary arteries. RESULTS: Compared to other groups, the pulmonary artery vessel wall in placebo group thickened and the vessel lumina was deflated. The pulmonary artery vessel wall and vessel lumina in bosentan group were restored to the status in the control group. Calponin was expressed in the pulmonary artery in all groups and the expression of calponin decreased with prolonged hypoxia. No significant difference was observed in the expression of calponin between bosentan group and control group. CONCLUSION: Bosentan promotes the synthesis of calponin in pulmonary arteries in rats with hypoxic pulmonary hypertension.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2010-01-30.通讯作者：张玉顺，主任医师，主要从事先天性心脏病介入治疗的研究Email:zys2889@sina.com 作者简介：何璐，硕士生Email:helu123.student@sina.com
更新日期/Last Update: 2010-10-27