AGEs对心肌微血管内皮细胞血管新生和管腔形成的影响
《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]
- 期数:
- 2011年第1期
- 页码:
- 42-45
- 栏目:
- 基础研究
- 出版日期:
- 2010-10-27
文章信息/Info
- Title:
- Effect of advanced glycation end products on angiogenesis and tube formation of microvascular endothelial cells
- Author(s):
- SU Yan-hui; LI Fei; LIU Nan; WANG Dong-juan; WANG Hai-chang
- Department of Cardiology, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, Shaanxi, China
- Keywords:
- advanced glycation end products; cardiac microvascular endothelial cell; angiogenesis; tubule-like structure formation
- 分类号:
- R587.2
- DOI:
- -
- 文献标识码:
- A
- 摘要:
- 目的: 观察糖基化终产物（advanced glycation end products，AGEs）对心肌微血管内皮细胞(cardiac microvascular endothelial cells，CMECs)增殖能力、迁移能力，以及血管新生和管腔形成的作用和影响。方法： 以不同浓度（100、200和400 mg/L）的AGEs作用于CMECs 48 h，分别采用MTT比色法检测细胞的增殖能力；用Transwell法检测细胞的迁移能力；用毛细血管管腔结构形成试验检测AGEs对血管新生的影响。结果： CMECs在以不同浓度（100、200及400 mg/L）的AGEs作用48 h后，MTT比色法检测显示，吸光值（分别为0.1195±0.0049、0.1422±0.0058及0.1783±0.0220）明显高于对照组（0.0955±0.0161，P<0.05，P<0.01）；Transwell法检测细胞数［分别为(24.89±6.234)、(32.89±6.990)及(55.56±10.27)个］均高于对照组［（13.89±4.622）个，P<0.05，P<0.01］；管样结构的长度［分别为(3.261±0.7016)、(4.737±1.129)及(6.687±1.308)mm/mm2］均高于对照组［(2.089±0.6723)mm/mm2，P<0.05，P<0.01］。结论： AGEs可以促进CMECs血管新生和管腔结构的形成，且与其浓度呈正相关。
- Abstract:
- AIM: To investigate the effects of the advanced glycation end products (AGEs) on the proliferation, migration and capillary tubule-like formation (TLS) in cardiac microvascular endothelial cells (CMECs). METHODS: CMECs were cultured with AGEs at different concentrations (100 mg/L, 200 mg/L, and 400 mg/L) for 48 h. MTT and transwell were used to detect the proliferation and migration and capillary tubule-like formation was examined to detect the angiogenesis. RESULTS: The A values of CMECs at the AGEs concentration of 100 mg/L, 200 mg/L and 400 mg/L after 48 h incubation were 0.1195±0.0049, 0.1422±0.0058 and 0.1783±0.0220, significantly higher than 0.0955±0.0161 (P<0.05, P<0.01). The migrations of CMECs (24.89±6.234, 32.89±6.990 and 55.56±10.27) were higher than 13.89±4.622 (P<0.05, P<0.01), and the tubule-like formation structures of CMECs were 3.261±0.7016, 4.737±1.129 and 6.687±1.308, higher than 2.089±0.6723 (P<0.05, P<0.01 vs. control group) in a concentration dependent manner. CONCLUSION: AGEs promote the proliferation, migration and tubule-like structure formation in a concentration-dependent manner.
参考文献/References
[1]杨启红，徐强，司良毅． 糖基化终产物对单核细胞源性巨噬细胞过氧化物酶体增殖物激活型受体7表达的影响[J]． 中华老年心脑血管病杂志， 2008， 10（3）：214-217．
[2] 尹志勇,魏丽萍,郝媛媛,等. LY333531对高糖所致心肌微血管内皮细胞通透性上调的拮抗作用[J]. 细胞与分子免疫学杂志, 2009, 25(4):303-305.
[3]Staton CA, Stribbling SM, Tazzyman S, et al. Current methods assaying angiogenes is in vitro and in vivo[J]. Int J Exp Pathol, 2004, 85(5):233-248.
[4] Takeuchi M, Yamagishi S. Possible involvement of advanced glycation end-products (AGEs) in the pathogenesis of Alzheimer’s disease[J]. Curr Pharm Des, 2008, 14(10):973-978.
[5] Huijberts MS, Schaper NC, Schalkwijk CG. Advanced glycation end products and diabetic foot disease[J]. Diabetes Metab Res Rev, 2008, 24(Suppl 1):S19-S24.
[6]Poul MA, Becerril B, Nielsen UB, et al. Selection of tumor-specific internalizing human antibodies from phage libraries[J]. J Mol Biol, 2000, 301(5):1149-1161.
[7]Du Y, Weed SA, Xiong WC, et al. Identification of a novel cortactin SH3 domain-binding protein and its localization to growth cones of cultured neurons[J]. Mol Cell Biol, 1998, 18(10):5838-5851.
[8]Sabbah HN, Sharov VG. Apoptosis in heart failure[J]. Prog Cardiolvasc Dis, 1998, 40(6):549-562.
[9]Ayalasomayajula SP, Kompella UB. Celecoxib, a selective cyclooxygenase-2 inhibitor, inhibits retinal vascular endothelial growth factor expression and vascular leakage in a streptozotocin-induced diabetic rat model[J]. Eur J Pharmaco, 2003, 458 (3):283-289.
备注/Memo
- 备注/Memo:
- 收稿日期：2010-02-08.通讯作者：王海昌，主任医师，主要从事冠心病基础研究 Email：wanghc@fmmu.edu.cn 作者简介：粟妍晖，硕士生Email:shiyao2357@sina.com
常用功能
统计/Statistics
- 摘要浏览/Viewed1267
- 评论/Comments
